Expression of differentiated embryo-chondrocyte expressed gene l in oral squamous cell carcinoma and its clinical significance

Abstract

Abstract: PURPOSE: To investigate the expression of differentiated embryo-chondrocyte expressed gene l(DEC1)in oral squamous cell carcinoma(OSCC)and its clinical significance. METHODS: Detection of the protein expression of DEC1 in tissue specimens from 80 primary OSCC patients and 30 cases of normal oral mucosa were performed using immunohistochemistry. SPSS10.0 software package was uses for statistical analysis. RESULTS: The result of immunohistochemistry showed that positive expression rate of DEC1 in OSCC tissues was 92.50%, 5.00% in normal oral mucosa tissue. The difference was statistically significant (P<0.05). In OSCC tissues, the difference in expression of DEC1 was statistically significant (P<0.05) between highly differentiated group and lowly differentiated group, between patients with lymph node metastasis and patients without lymph node metastasis. CONCLUSIONS: The significantly increased DEC1 gene may be associated with the tumorigenesis and development of OSCC.

Key words: Differentiated embryo-chondrocyte expressed gene l, Oral squamous cell carcinoma, Immunohistochemistry

CLC Number:

R739.8

YOU Jing-min, SU Tong, XIA Kun, LIN Lu, LIU qiong, ZHU Teng-fei. Expression of differentiated embryo-chondrocyte expressed gene l in oral squamous cell carcinoma and its clinical significance[J]. Shanghai Journal of Stomatology, 2016, 25(2): 217-220.

References

Shen M, Kawamoto T, Yan W, et al. Molecular characterization of the novel basic helix-loop-helix protein DEC1 expressed in differentiated human embryo chondrocytes. Biochem Biophys Res Commun, 1997, 236(2): 294-298.
Wu Y, Sato F, Yamada T, et al. The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer. Int J Oncol, 2012, 41(4): 1337-1346.
Liu Y, Miao Y, Wang J, et al. DEC1 is positively associated with the malignant phenotype of invasive breast cancers and negatively correlated with the expression of claudin-1. Int J Mol Med, 2013, 31(4): 855-860.
Noshiro M, Usui E, Kawamoto T, et al. Liver X receptors (LXRalpha and LXRbeta) are potent regulators for hepatic Dec1 expression . Genes Cells, 2009, 14(1): 29-40. Jemal A, Bray F, Center MM, et al. Global cancer statistics . CA Cancer J Clin, 2011, 61(2): 69-90.
Wiltshire S, Frayling TM, Hattersley AT, et al. Evidence for linkage of stature to chromosome 3p26 in a large U.K. Family data set ascertained for type 2 diabetes. Am J Hum Genet, 2002, 70(2): 543-546.
Teramoto M, Nakamasu K, Noshiro M, et al. Gene structure and chromosomal location of a human bHLH transcriptional factor DEC1 x Stra13 x SHARP-2/BHLHB2 . J Biochem, 2001, 129(3): 391-396.
Yamada K, Miyamoto K. Basic helix-loop-helix transcription factors, BHLHB2 and BHLHB3; their gene expressions are regulated by multiple extracellular stimuli . Front Biosci, 2005, 10: 3151-3171.
Davis RL, Turner DL. Vertebrate hairy and enhancer of split related proteins: transcriptional repressors regulating cellular differentiation and embryonic patterning . Oncogene, 2001, 20(58): 8342-8357.
Li Y, Zhang H, Xie M, et al. Abundant expression of Dec1/stra13/sharp2 in colon carcinoma: its antagonizing role in serum deprivation-induced apoptosis and selective inhibition of procaspase activation . Biochem J, 2002, 367(Pt 2): 413-422.
Giatromanolaki A, Koukourakis MI, Sivridis E, et al. DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer . J Pathol, 2003, 200(2): 222-228.
Zheng Y, Shi X, Wang M, et al. The increased expression of DEC1 gene is related to HIF-1alpha protein in gastric cancer cell lines . Mol Biol Rep, 2012, 39(4): 4229-4236.
Yoon DY, Buchler P, Saarikoski ST, et al. Identification of genes differentially induced by hypoxia in pancreatic cancer cells . Biochem Biophys Res Commun, 2001, 288(4): 882-886.
Bhawal UK, Ito Y, Tanimoto K, et al. IL-1beta-mediated up-regulation of DEC1 in human gingiva cells via the Akt pathway . J Cell Biochem, 2012, 113(10): 3246-3253.
Shi XH, Zheng Y, Sun Q, et al. DEC1 nuclear expression: a marker of differentiation grade in hepatocellular carcinoma . World J Gastroenterol, 2011, 17(15): 2037-2043.
Liu Y, Wang L, Lin XY, et al. The transcription factor DEC1 (BHLHE40/STRA13/SHARP-2) is negatively associated with TNM stage in non-small-cell lung cancer and inhibits the proliferation through cyclin D1 in A549 and BE1 cells. Tumour Biol, 2013, 34(3): 1641-1650.

[1]	DONG Lin-sheng, TANG Jin, CHENG Wei, RAN Jing-xuan, LIU Zhao-bo, LI Ya-dong. Expression and prognostic value of IL-6 and β-catenin in oral squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2023, 32(3): 280-286.
[2]	HU Xiao-yuan, WANG Ning, ZHANG Xiao-ye, YANG Qian, GAO Fei, WANG Cheng-qin, SHANG Wei, XIANG Feng-gang, FENG Yuan-yong. Chemerin mediated neutrophil infiltration in oral squamous cell carcinoma and predicted poor prognosis [J]. Shanghai Journal of Stomatology, 2023, 32(2): 158-165.
[3]	GUO Meng-yao, LI Kai-yu, NIE Min-hai, LIU Xu-qian. Effect of up-regulation of Decorin on expression of EGFR, C-Myc and p21 in nude mice with oral squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2023, 32(1): 40-46.
[4]	QIAN Yi, DENG Chao, XU Xiao-bo, CHEN Ye-jun, HU Yun-ping, HONG Li-lin, FENG Da-jun. Clinical analysis of immune function, intestinal flora changes in patients with oral squamous carcinoma before, after treatment [J]. Shanghai Journal of Stomatology, 2022, 31(5): 523-529.
[5]	WEN Xu-tao, MA Zhen, ZHANG Chong, Tao Shi-cheng, CHEN Xing-jin, MAI Hua-ming. Maintenance of the stemness in CD133⁺ primary oral squamous cell carcinoma cells under different culture conditions [J]. Shanghai Journal of Stomatology, 2022, 31(4): 343-348.
[6]	QIN Zhi-ming, JIE Wei-ping, CHI Yan-ting, LI Hong-feng, LI Bin-bin. Preliminary establishment of an unsupervised quantification model of Ki-67 proliferation index based on local variable threshold method [J]. Shanghai Journal of Stomatology, 2022, 31(3): 248-254.
[7]	CHEN Juan, CAO Ruo-yan, LIU Yang, HONG Peng-yu, TANG Zhan-gui. Expression of TNFAIP6 in oral verrucous carcinoma and oral squamous cell carcinoma and its relationship with clinicopathology [J]. Shanghai Journal of Stomatology, 2022, 31(2): 167-172.
[8]	MAIERHABA·Maihemuti, BAI Jie, RUSITANMU·Yimiti, YAO Zhi-tao. Influencing factors of nutritional status and change in 50 patients with oral squamous cell carcinoma during treatment [J]. Shanghai Journal of Stomatology, 2022, 31(2): 205-210.
[9]	TAN Yi-ran, ZHAO Tong-chao, JU Wu-tong, ZHONG Lai-ping. Experimental investigation of vincristine on chemosensitivity through Stathmin regulation in oral squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2022, 31(1): 1-5.
[10]	XIE Fei, QIN Xing, TONG Tong, JIANG Ying-ying, ZHANG Jian-jun. Insight into the role of IGF2BP1 in drug resistant mechanism of oral squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2021, 30(5): 456-461.
[11]	LI Lei, LI Xiao-dong, ZHUANG Qian-wei, GE Liang-yu, LI Zhi-ping, GU Qian-ping, BAI Yu-ting, MENG Jian. Analysis of curative effect of ultrasonic hyperthermia combined with TPF chemotherapy on 19 elderly with advanced oral squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2021, 30(5): 543-547.
[12]	SHAO Xiao-jun, ZHU Qiao, LIU You, HAN Xiao-dong, LIN Xiao-zhen, XI Qing. Correlation of betel nut chewing and clinicopathologic factors of oral squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2021, 30(3): 268-272.
[13]	JIN Neng-hao, NAN Xin-rong, YAN Xing-quan. Expression and clinical significance of BTG-1 in 78 patients with oral squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2021, 30(2): 140-144.
[14]	XUE Jia-yi, ZHU Xiao-yan, WEI Chang-lei, SUN Pei, ZHANG Hui, DENG Jing. Study of salivary metabonomics in patients with oral squamous cell carcinoma and leukoplakia [J]. Shanghai Journal of Stomatology, 2021, 30(2): 201-205.
[15]	WANG Ke, PENG Guo-guang, TAN Yu-lian, HE Shan-zhi, LUO Cui-fen. MiR-99a inhibits proliferation of oral squamous cell carcinoma by targeting mTOR pathway [J]. Shanghai Journal of Stomatology, 2021, 30(1): 44-49.