Coculture of stem cells from the apical papilla and human umbilical vein endothelial cells enhances the angiogenic potential of dental pulp tissues

Shanghai Journal of Stomatology ›› 2014, Vol. 23 ›› Issue (5): 547-552.

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Coculture of stem cells from the apical papilla and human umbilical vein endothelial cells enhances the angiogenic potential of dental pulp tissues

YUAN Chang-yong^{1, 2, 3}, WANG Peng-lai^{1, 2}, ZHANG Cheng-fei³, LIU Zong-xiang⁴, LUO San-lian⁵

1.Dental Implant Center, Xuzhou Medical College Affiliated Xuzhou Stomatological Hospital. Xuzhou 221002, Jiangsu Province; 2.Xuzhou Medical College School of Stomatology. Xuzhou 221004, Jiangsu Province; 3. Faculty of Dentistry, The University of Hong Kong. Hong Kong 999077; 4. Department of Periodontics, Xuzhou Medical College Affiliated Xuzhou Stomatological Hospital. Xuzhou 221002, Jiangsu Province; 5. Department of Stomatology, Xuzhou Medical College Second Affiliated Hospital. Xuzhou 221006, Jiangsu Province, China

Received:2014-01-06 Revised:2014-03-13 Online:2014-10-20 Published:2015-02-04
Supported by:
; Supported by Science and Technology Planning Project of Xuzhou City (XM13B094) and Foundation for Young Reserve Medical Talents of Xuzhou City (2014014)

Abstract

Abstract: PURPOSE: To observe the effect of coculture of stem cells from the apical papilla (SCAPs) and human umbilical vein endothelial cells (HUVECs) on the angiogenic potential of dental pulp tissues. METHODS: SCAPs were incubated in osteo/odontogenic, adipogenic, neurogenic induction medium and α-MEM medium, whose multilineage differentiation capacities were confirmed using alizarin red staining, oil red O staining and βⅢ-tubulin immunofluorescent staining. The tubular length, branching points number and junctional areas were detected after 3, 6, 9 h since cells were seeded onto matrigel, and the data was analyzed using SPSS 16.0 software package. RESULTS: SCAPs in the experimental groups were detected having more lipid droplets, mineralization nodules and neuron-like cells. Coculture of SCAPs and HUVECs formed more vessel-like structures in tubular formation assay. CONCLUSIONS: SCAPs are capable of differentiating into fat, bone, and nerve-like cells in vitro. Coculture of SCAPs and HUVECs can enhance the angiogenic potential of dental pulp tissues.

Key words: Stem cells from the apical papilla, Human umbilical vein endothelial cells, Dental pulp regeneration, Angiogenesis

YUAN Chang-yong, WANG Peng-lai, ZHANG Cheng-fei, LIU Zong-xiang, LUO San-lian. Coculture of stem cells from the apical papilla and human umbilical vein endothelial cells enhances the angiogenic potential of dental pulp tissues[J]. Shanghai Journal of Stomatology, 2014, 23(5): 547-552.

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Coculture of stem cells from the apical papilla and human umbilical vein endothelial cells enhances the angiogenic potential of dental pulp tissues

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