Effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells

Abstract

Abstract: PURPOSE: To explore the effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells (SACC-83, SACC-LM). METHODS: Different doses of radiation (0, 2, 4, 6, 8, 10 Gy) were applied to SACC-83, SACC-LM cells and the cells were continued to culture for 48 h. CCK-8 test, flow cytometry(FCM) and cell scratch experiment were used to observe cell proliferation, apoptosis and cell migration. SPSS19.0 software package was used for data analysis. RESULTS: The effect of radiation on SACC-LM cells survival rate, cell apoptosis, heteroploid and cell migration ability were significantly greater than that on SACC-83 cells (P<0.05). In SACC-83 cells, compared with other doses of radiation, 6 Gy of irradiation was the most sensitive on cell survival rate, cell apoptosis, heteroploid and cell migration ability. CONCLUSIONS: Radiation sensitivity of SACC-LM was greater than that of SACC-83 cells. With 6 Gy of radiation, changes of biology in SACC-83 cells most significant than other doses of radiation.

Key words: Salivary gland, Adenoid cystic carcinoma, Radiation sensitivity, Cell apoptosis.

CLC Number:

R730.261

HUANG Yu-meng, WANG Ying, LI Yong, LIU Ping, LIU Xian-feng, ZHAO Hong-wei. Effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells[J]. Shanghai Journal of Stomatology, 2016, 25(4): 403-408.

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