Expression of HIF-1α in the genioglossus associated with induced bilateral intermittent nasal obstruction in young rats

Abstract

Abstract: PURPOSE: To investigate the expression of HIF-1α in the genioglossus associated with induced bilateral intermittent nasal obstruction in young rats. METHODS: Thirty 4-week-old SD rats were employed and equally divided into 3 groups. In group A, both nostrils were occluded by nose plugs. In group B, the right nostril was occluded. In group C, no obstruction of the nose was performed as control group. The obstruction time was from 8 am to 12 am everyday, and the period was 21 d and 55 d. The genioglossus was taken for HE, and immunohistochemical staining was used to detect the expression of HIF-1α. The data was analyzed with SPSS 13.0 software package. RESULTS: The rats were sacrificed at the 21th day and 55th day, respectively. The expression of HIF-1α in group A was significantly higher than that in group B and group C, and became stronger with the increasing of obstruction time. CONCLUSIONS: Oral breathing caused by bilateral intermittent nasal obstruction in young rats results in overexpression of HIF-1α in the genioglossus.

Key words: Nasal obstruction, Genioglossus, HIF-1α

CLC Number:

R782.2

LIU Jiong, CHEN Jin-dong, NIE Ping, SHENG Xiao, ZHAO Yan-hui, XU Xiao-long, TAO Li, ZHU Min. Expression of HIF-1α in the genioglossus associated with induced bilateral intermittent nasal obstruction in young rats[J]. Shanghai Journal of Stomatology, 2014, 23(2): 138-142.

References

[1] Posnick JC, Agnihotri N. Consequences and management of nasal airway obstruction in the dentofacial deformity patient [J]. Curr Opin Otolaryngol Head Neck Surg, 2010, 18(4): 323-331.
[2] Defabjanis P. Impact of nasal airway obstruction on dentofacial development and sleep disturbances in children: preliminary notes [J]. J Clin Pediatr Dent, 2003, 27(2): 95-100.
[3] Ackerman RI, Klapper L. Tongue position and open-bite: the key roles of growth and the nasopharyngeal airway [J]. ASDC J Dent Child, 1981, 48(5): 339-345.
[4] Gaillard-Perera H, Farah A, Gaillard A. Respiratory pathology of sleep and the upper airways [J]. Rev Stomatol Chir Maxillofac, 1992, 93(2): 130-137.
[5] McNamara JA. Influence of respiratory pattern on craniofacial growth [J]. Angle Orthod, 1981, 51(4): 269-300.
[6] Harvold EP, Tomer BS, Vargervik K, et al. Primate experiments on oral respiration [J]. Am J Orthod, 1981, 79(4): 359-372.
[7] Edge D, Bradford A, Jones JF, et al. Chronic intermittent hypoxia alters genioglossus motor unit discharge patterns in the anaesthetized rat [J]. Adv Exp Med Biol, 2012, 758: 295-300.
[8] Scarano E, Fetoni AR, Picciotti P, et al. Can chronic nasal obstruction cause dysfunction of the paratubal muscles and otitis media? An experimental study in developing Wistar rats [J]. Acta Otolaryngol, 2003, 123(2): 288-291.
[9] Ryan S, Taylor CT, McNicholas WT. Systemic inflammation: a key factor in the pathogenesis of cardiovascular complications in obstructive sleep apnoea syndrome?[J]. Postgrad Med J, 2009, 85(1010): 693-698.
[10] Musso G, Olivetti C, Cassader M, et al. Obstructive sleep apnea-hypopnea syndrome and nonalcoholic fatty liver disease: emerging evidence and mechanisms[J]. Semin Liver Dis, 2012, 32(1):49-64.
[11] Minchenko A, Leshchinsky I, Opentanova I, et al. Hypoxia-inducible factor-1-mediated expression of the 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-3 (PFKFB3) gene. Its possible role in the Warburg effect[J]. J Biol Chem, 2002, 277(8): 6183-6187.
[12] Royer C, Lachuer J, Crouzoulon G, et al. Effects of gestational hypoxia on mRNA levels of Glut3 and Glut4 transporters, hypoxia inducible factor-1 and thyroid hormone receptors in developing rat brain [J]. Brain Res, 2000, 856(1-2): 119-128.
[13] Yang X, Wei A, Liu Y, et al. IGF-1 protects retinal ganglion cells from hypoxia-induced apoptosis by activating the Erk-1/2 and Akt pathways [J]. Mol Vis, 2013, 19: 1901-1912.
[14] Kovaleva IuO, Artem'eva MM, Medvedev OS, et al. Chronic administration of estrogen receptors antagonist reduces degree of hypoxia-induced pulmonary hypertension caused by chronic injections of estrogen in ovariectomised female Wistar rats [J]. Eksp Klin Farmakol, 2013, 76(7): 15-18.
[15] Vijayasarathy C, Damle S, Prabu SK, et al. Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia [J]. Eur J Biochem, 2003, 270(5): 871-879.