Effect of chronic sleep deprivation on condylar cartilage in rats

doi:10.19439/j.sjos.2022.02.007

Abstract

Abstract: PURPOSE: The aim of this study was to investigate the morphological changes of condylar cartilage of temporomandibular joint (TMJ) and the expression changes of IL-1β,TNF-α,IGF-1 and VEGF in condylar cartilage of TMJ by establishing a chronic sleep deprivation model in rats. METHODS: Sixty rats were randomly divided into experimental group, control group and recovery group. Modified multiple platforms method (MMPM) was used to build chronic sleep deprivation models in experimental and recovery groups. Rats in the recovery group received 1 week of cage feeding after sleep deprivation. H-E staining was used to observe morphological change of the condyle. Immunohistochemical method was performed to detect the changes of IL-1β, TNF-α, IGF-1 and VEGF. The data was processed by using SPSS 23.0 software package. RESULTS: MMPM can establish chronic sleep deprivation model effectively. H-E staining showed condylar cartilage of the experimental group was split stripped, and the boundaries of cartilage cell layer became blurred. Compared with the control group, the recovery group had less cracks in the fibrous layer or some of the cracks were occupied by fibrous tissue. Immunohistochemistry showed that the positive expression intensity of IL-1β and TNF-α in the experimental group was significantly higher than in the control group (P<0.05), the positive expression intensity in the recovery group was significantly lower than in the experimental group(P<0.05). The positive expression intensity of IGF-1 and VEGF in the experimental group was significantly higher than in the control group(P<0.05). The expression of IGF-1 and VEGF decreased significantly in the recovery group which received sleep deprivation no more than 3 weeks(P<0.05). CONCLUSIONS: Chronic sleep deprivation can increase the expression of IL-1β, TNF-α and VEGF in condylar cartilage and aggravate osteoarthritis. Chronic sleep deprivation can lead to increase of IGF-1 in condylar cartilage tissue, which plays a crucial role in protecting and promoting the reconstruction of condylar cartilage. After chronic sleep deprivation, the expressions of IL-1β, TNF-α, IGF-1 and VEGF in the condylar cartilage of rats were decreased after 1 week of recovery, and the condylar cartilage underwent restorative reconstruction.

Key words: Chronic sleep deprivation, Interleukin-1β, Tumor necrosis factor α, Insulin-like growth factor, Vascular endothelial growth fact

CLC Number:

R782.6

BAI Yue-hui, LIU Yang, CUI Yu-lan, JIANG Shan-shan, SHANG Qing-long, ZHAO Chen. Effect of chronic sleep deprivation on condylar cartilage in rats[J]. Shanghai Journal of Stomatology, 2022, 31(2): 148-155.

References

[1] Zhang ZK, Ma XC, Gao S, et al.Studies on contributing factors in temporomandibular disorders[J]. Chin J Dent Res, 1999, 2(3-4): 7-20.
[2] Turp JC, Schindler H, 刘晓东, 等. 颞下颌关节病的病因问题:咬合因素的流行病学和病因学依据[J]. 实用口腔医学杂志, 2015, 31(3): 417-424.
[3] 胡欣欣, 朱耀旻, 何柳婷, 等. 109 例颞下颌关节紊乱病相关因素分析[J]. 上海口腔医学, 2017, 26(2): 213-216.
[4] 李涛, 黎钢. 关节腔注射治疗对不同年龄段颞下颌关节骨关节炎患者的疗效评价[J]. 上海口腔医学, 2015, 24(3): 356-360.
[5] 张颖, 卢锦芬, 张清彬. 改良后牙咬合板治疗颞下颌关节盘不可复性前移位的效果评价[J]. 上海口腔医学, 2021, 30(2): 210-213.
[6] 谷志远. 颞下颌关节紊乱病病因学研究[J]. 中国实用口腔科杂志, 2009, 2(3): 129-131.
[7] Yatani H, Studts J, Cordova M, et al.Comparison of sleep quality and clinical and psychologic characteristics in patients with temporomandibular disorders[J]. J Orofac Pain, 2002, 16(3): 221-228.
[8] Riley JL 3rd, Benson MB, Gremillion HA, et al. Sleep disturbance in orofacial pain patients: pain-related or emotional distress?[J].Cranio, 2001, 19(2) : 106-113.
[9] Harness DM, Donlon WC, Eversole LR.Comparison of clinical characteristics in myogenic, TMJ internal derangement and atypical facial pain patients[J]. Clin J Pain, 1990, 6(1): 4-17.
[10] Geng W, Wu G, Huang F, et al.Sleep deprivation induces abnormal bone metabolism in temporomandibular joint[J]. Int J Clin Exp Med, 2015, 8(1): 395-403.
[11] 朱勇, 马川, 陈虹瑜, 等. 慢性睡眠限制状态下大鼠髁突软骨MMP-1,MMP-13表达的变化[J]. 现代生物医学进展, 2015, 15(25): 4860-4865.
[12] Suchecki D, Palma BD, Tufik S.Sleep rebound in animals deprived of paradoxical sleep by the modified multiple platform method[J]. Brain Res, 2000, 875(1-2) : 14-22.
[13] 黄建欧, 赵忠新.大鼠睡眠剥夺方法的研究进展[J].中华神经医学杂志, 2004, 3(3): 229-231.
[14] 马川, 陈虹瑜, 赵华强, 等. 睡眠剥夺对大鼠颞下颌关节髁突组织p38信号通路相关蛋白表达的影响[J]. 山东医药, 2014, 54(6): 21-23.
[15] 傅开元. 2014年新版国际颞下颌关节紊乱病分类及诊断标准解读[J]. 中华口腔医学杂志, 2017, 52(6): 374-376.
[16] 傅开元. 颞下颌关节紊乱病的分类与诊断[C]//王美青.学[M].北京:人民卫生出版社, 2018.
[17] 魏欣, 杨禾丰, 胡瑜, 等. 小型实验动物颞下颌关节骨关节炎动物模型的研究进展[J]. 医学综述, 2019, 25(7): 1375-1379.
[18] Dworkin SF, Huggins KH, LeResche L, et al. Epidemiology of signs and symptoms in temporomandibular disorders: clinical signs in cases and controls[J]. J Am Dent Assoc, 1990, 120(3): 273-281.
[19] Ding F, Wang J, Zhu G, et al.Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes[J]. Am J Transl Res, 2017, 9(2): 316-329.
[20] 孙晶, 陈永进, 高晖, 等. 心理应激状态对大鼠颞下颌关节组织形态的影响[J].牙体牙髓牙周病学杂志, 2009, 19(4): 211-214.
[21] Huey DJ, Hu JC, Athanasiou KA.Unlike bone, cartilage regeneration remains elusive[J]. Science, 2012, 338(6109): 917-921.
[22] Aborehab NM, El Bishbishy MH, Refaiy A, et al.A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis[J]. BMC Complement Altern Med, 2017, 17(1): 495-503.
[23] Altman RD, Manjoo A, Fierlinger A, et al.The mechanism of action for hyaluronic acid treatment in the osteoarthritic knee: a systematic review[J]. BMC Musculoskelet Disord, 2015, 16: 321-330.
[24] Khan Z, Agarwal NB, Bhurani D, et al.Risk factors for hematopoietic stem cell transplantation-associated bone loss[J]. Transplant Cell Ther, 2021, 27(3): 212-221.
[25] 牟方政, 李荣亨. 胰岛素样生长因子1与骨关节炎研究进展[J]. 中国老年学杂志, 2012, 32(22): 5089-5092.
[26] 王轲, 赵兵杰, 陈云, 等. 慢性睡眠剥夺对大鼠颞下颌关节的影响[J]. 口腔生物医学, 2020, 11(4): 246-251.
[27] Goodrich LR, Hidaka C, Robbins PD, et al.Genetic modification of chondrocytes with insulin-like growth factor-1 enhances cartilage healing an equine model[J]. J Bone Joint Surg Br, 2007, 89(5): 672-685.
[28] Garcia-Ramirez M, Toran N, Andaluz P, et al.Vascular endothelial growth factor is expressed in human fetal growth cartilage[J]. J Bone Miner Res, 2000, 15(3): 534-540.
[29] Wang S, Zhou C, Zheng H, et al.Chondrogenic progenitor cells promote vascular endothelial growth factor expression through stromal-derived factor-1[J]. Osteoarthritis Cartilage, 2017, 25(5): 742-749.