Evaluation of osseointegration of domestic porous tantalum-niobium alloy

doi:10.19439/j.sjos.2018.05.006

Abstract

Abstract: PURPOSE: To evaluate the osseointegration of domestic porous tantalum-niobium(PTa-Nb) alloy. METHODS: A total of 36 adult New Zealand rabbits were selected and divided into 3 groups. Pta-Nb rods(3.5 mm×10 mm) were implanted into the femoral condyle of each rabbit.The rabbits were sacrificed successively at 4,8,12 weeks after operation. X-ray, hard tissue slices stained with toluidine blue, scanning electron microscope(SEM), X-ray energy disperse spectroscopy(XEDS) and push-out test were used to test the osseointegration of PTa-Nb. SPSS19.0 software package was used for statistical analysis. RESULTS: X-ray films showed no obvious inflammation,as well as implants loosing and bone resorption. Density of the bone around implants increased. Hard tissue slices displayed chimeric shape in the implant-bone interface. New bone contacted directly to the surface of PTa-Nb and got more and more closer with the increase of healing time. Osteoid formed in the inner pores at 4 weeks after operation,and more mature bone tissue grew into inner part of PTa-Nb at 12 weeks. SEM showed bone tissue was more denser and closer to the Pta-Nb,which was similar to the results of hard tissue slices. XEDS analysis showed the percent content of the calcium and phosphate of the tissue within PTa-Nb increased gradually in the three groups.The ratio of Ca/P at 8 and 12 weeks was significantly higher than at 4 weeks (P<0.05); Push-out test showed the shear-strength of PTa-Nb implants increased from (8.26±0.75) MPa at 4 weeks to (21.04±1.46) MPa at 12 weeks (P<0.05). CONCLUSIONS: The domestic PTa-Nb alloy has good osseointegration with both bone tissue ongrowth and ingrowth,which may be a high potential biomimetic bone material.

Key words: Porous tantalun-niobium, Osseointegration, Biomimetic bone material, Rabbits

CLC Number:

R738.1

LIU Qiang, ZHANG Yi-neng, LI Hui-jun, LIU Jue, RUAN Jian-ming, ZHOU Xiong-wen. Evaluation of osseointegration of domestic porous tantalum-niobium alloy[J]. Shanghai Journal of Stomatology, 2018, 27(5): 477-481.

References

[1] Balla VK, Bodhak S, Bose S, et al.Porous tantalum structures for bone implants: fabrication, mechanical and in vitro biological properties[J]. Acta Biomater, 2010, 6(8): 3349-3359.
[2] Yazdimamaghani M, Razavi M, Vashaee D, et al.Porous magnesium-based scaffolds for tissue engineering[J]. Mater Sci Eng C Mater Biol Appl, 2017, 71: 1253-1266.
[3] Tsao AK, Roberson JR, Christie MJ, et al.Biomechanical and clinical evaluations of a porous tantalum implant for the treatment of early-stage osteonecrosis[J]. J Bone Joint Surg Am, 2005, 87(Suppl 2): 22-27.
[4] Gerscovich D, Schwing C, Unger A.Long-term results of a porous tantalum monoblock tibia component: clinical and radiographic results at follow-up of 10 years[J]. Arthroplast Today, 2017, 3(3): 192-196.
[5] 王汇丰, 阮建明, 李婧, 等.多孔钽铌合金及其制备方法: 中国, 103805798 [P]. 2014.
[6] Wang H, Li J, Yang H, et al.Fabrication, characterization and in vitro biocompatibility evaluation of porous Ta-Nb alloy for bone tissue engineering[J]. Mater Sci Eng C Mater Biol Appl, 2014, 40: 71-75.
[7] Chen Q, Thouas GA.Metallic implant biomaterials[J]. Mater Sci Eng R Rep, 2015, 87: 1-57.
[8] Wang N, Li H, Wang J, et al.Study on the anticorrosion, biocompatibility, and osteoinductivity of tantalum decorated with tantalum oxide nanotube array films[J]. ACS Appl Mater Interfaces, 2012, 4(9): 4516-4523.
[9] Mohandas G, Oskolkov N, Mcmahon MT, et al.Porous tantalum and tantalum oxide nanoparticles for regenerative medicine[J].Acta Neurobiol Exp (Wars), 2014, 74(2):188-196.
[10] Annunziata M, Guida L.The effect of titanium surface modifications on dental implant osseointegration[J]. Front Oral Biol, 2015, 17: 62-77.
[11] Levine BR, Sporer S, Poggie RA, et al.Experimental and clinical performance of porous tantalum in orthopedic surgery[J]. Biomaterials, 2006, 27(27): 4671-4681.
[12] Jonitz A, Lochner K, Lindner T, et al.Oxygen consumption, acidification and migration capacity of human primary osteoblasts within a three-dimensional tantalum scaffold[J]. J Mater Sci Mater Med, 2011, 22(9): 2089-2095.
[13] Welldon KJ, Atkins GJ, Howie DW, et al.Primary human osteoblasts grow into porous tantalum and maintain an osteoblastic phenotype[J].J Biomed Mate Res A, 2008, 84(3): 691-701.
[14] Wang Q, Zhang H, Li Q, et al.Biocompatibility and osteogenic properties of porous tantalum[J]. Exp Ther Med, 2015, 9(3): 780-786.
[15] Bobyn JD, Stackpool GJ, Hacking SA, et al.Characteristics of bone ingrowth and interface mechanics of a new porous tantalum biomaterial[J].J Bone Joint Surg Br, 1999, 81(5): 907-914.
[16] 栗振宝, 孙庆, 潘力. 软骨钙化的X射线能谱半定量分析[J]. 电子显微学报, 1994, 13(5): 394.
[17] 陈勤, 何季平, 郑启新, 等. 多孔磷酸三钙陶瓷骨内植入后的超微结构研究[J]. 中国生物医学工程学报, 1999, 18(3): 262-272.
[18] Perényi J, Bene L, Radnai M, et al.Model investigation of push-out test used for quantitative evaluation of dental implant osseointegration[J]. Fogorv Sz, 2002, 95(3): 105-111.
[19] Smith JO, Sengers BG, Aarvold A, et al.Tantalum trabecular metal-addition of human skeletal cells to enhance bone implant interface strength and clinical application[J]. J Tissue Eng Regen Med, 2014, 8(4): 304-313.
[20] Itälä AI, Ylänen HO, Ekholm C, et al.Pore diameter of more than 100 microm is not requisite for bone ingrowth in rabbits[J]. J Biomed Mater Res, 2001, 58(6): 679-683.
[21] Jones AC, Arns CH, Sheppard AP, et al.Assessment of bone ingrowth into porous biomaterials using MICRO-CT[J]. Biomaterials, 2007, 28(15): 2491-2504.
[22] Li JP, Habibovic P, van den Doel M, et al. Bone ingrowth in porous titanium implants produced by 3D fiber deposition[J]. Biomaterials, 2007, 28(18): 2810-2820.