Investigation of the effect of nuclear factor κB on inflammatory cell recruitment phenotype of oral cancer associated macrophage

doi:10.19439/j.sjos.2017.02.005

Abstract

Abstract: PURPOSES: To explore the ability of nuclear factor κB (NFκB) in sustaining inflammatory cell recruitment phenotype of oral cancer associated macrophages, by using NFκB inhibitor(-Bay11-7082). METHODS: By primary culture, murine macrophages were harvested. Cal27 conditioned medium (CM) and Bay11-7082 were applied for stimulation of the macrophages. RT-PCR and ELISA were used for detecting the inflammatory cell recruitment related chemotactic factors. GraphPadPrism5 was used for statistical analysis. RESULTS: Bay11-7082 prevented the contour change into a spindle shape via Cal 27 CM. It also attenuated MCP-1, GM-CSF, MCP-5 and CCL-5 mRNA increase after Cal 27 CM stimulation (P<0.05). At protein level, impeding NFκB activation could significantly prevent MCP-1 and GM-CSF secretion from oral cancer associated macrophage (P<0.001). CONCLUSIONS: NFκB signaling may play a key role in sustaining the inflammatory cell recruitment phenotype of oral cancer associated macrophages.

Key words: Inflammatory cell recruitment, Mouth neoplasm, Nuclear factor κB, Macrophage

CLC Number:

R739.8

ZHOU Xiao-tian, CHEN Dong, JIN Zhen-ning, HUANG Ke, LIN Jie, MEN Yi, LI Chun-jie. Investigation of the effect of nuclear factor κB on inflammatory cell recruitment phenotype of oral cancer associated macrophage[J]. Shanghai Journal of Stomatology, 2017, 26(2): 151-155.

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