Effect of tobacco extracts on human gingival fibroblasts' proliferation and fibronectin expression

doi:10.19439/j.sjos.2017.01.002

Abstract

Abstract: PURPOSE: To investigate the influence and mechanism of smokeless tobacco (ST) extracts on proliferation and fibronectin expression of human gingival fibroblasts (HGFs) adhered to titanium plates. METHODS: The cultured primary HGFs with ST of different concentrations for a period of 2h and 2, 4, 6, 8 d was separately conducted; the cell proliferation and the adhesion was assayed using CCK-8 method; enzyme-linked immunosorbent assay was used to determine the content of supernatant fibronectin (FN) at different times. The data was analyzed with SPSS 19.0 software package. RESULTS: With the increase of ST concentration, the cell adherence rate decreased accordingly. With ST concentration of 5.0 g/L and 10 g/L, the adhesion rate was (34.316±7.725)% and (25.478±10.651)%, respectively, which was significantly lower than that of the control group (100%) (P<0.01). Cell proliferation on the titanium plate started at 2 d and 4 d and the cell abundance of the control group was significantly greater than that of ST group (P<0.05) with concentrations of 2.5, 5.0, 10 g/L; The cell abundance of the control group was significantly greater than that of ST group (P<0.05) with concentrations of 0.625-10 g/L on the 6th and 8th day. In 0.625, 1.25, 2.5 g/L ST group, FN concentration was (69.352±31.640), (23.595±8.625) and (7.292±2.865) ng/mL, respectively, which was significantly lower than that of the control group (142.188 ± 28.126) ng/mL (P<0.05). CONCLUSIONS: ST can significantly inhibit the proliferation and adhesion of HGFs on pure titanium surface, and the mechanism may be related to decrease of FN secreted by HGFs.

CLC Number:

R783.4

SHAO Jun-ya, WANG Jian-zhong, XU Meng-rong, ZHOU Lan-fang. Effect of tobacco extracts on human gingival fibroblasts' proliferation and fibronectin expression[J]. Shanghai Journal of Stomatology, 2017, 26(1): 7-11.

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