PRRX1对唾液腺腺样囊性癌细胞自噬的影响

doi:10.19439/j.sjos.2021.05.006

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (5): 477-480.doi: 10.19439/j.sjos.2021.05.006

PRRX1对唾液腺腺样囊性癌细胞自噬的影响

高洁^*, 姜雅萍^*, 马康傑, 赵保东^#, 邓婧^#

青岛大学附属医院,青岛大学口腔医学院,山东青岛 266003

收稿日期:2021-01-12 修回日期:2021-04-01 出版日期:2021-10-25 发布日期:2021-11-08
通讯作者: 邓婧,E-mail:dengjing3333@126.com;赵保东,E-mail:zbd315@sina.com。^#共同通信作者
作者简介:高洁（1995-）,女,在读硕士研究生,E-mail:1256353889@qq.com;姜雅萍（1984-）,女,博士,主治医师,E-mail：jyp6817@163.com。^*并列第一作者
基金资助:
山东省自然科学基金（ZR2017BH089）

Effect of PRRX1 on autophagy of salivary adenoid cystic carcinoma cells

GAO Jie, JIANG Ya-ping, MA Kang-jie, ZHAO Bao-dong, DENG Jing

Affiliated Hospital of Qingdao University, School of Stomatology of Qingdao University. Qingdao 266003, Shandong Province, China

Received:2021-01-12 Revised:2021-04-01 Online:2021-10-25 Published:2021-11-08

摘要/Abstract

摘要： 目的: 探讨配对相关同源框 1（paired related homeobox1,PRRX1）对唾液腺腺样囊性癌（salivary adenoid cystic carcinoma,SACC）细胞自噬的影响。方法: 用PRRX1过表达慢病毒载体及PRRX1慢病毒空载体转染SACC细胞后,应用Western蛋白免疫印迹法检测转染效果。利用透射电子显微镜观察自噬小体,通过细胞免疫荧光和蛋白免疫印迹法检测自噬标志物（LC3-II/Beclin1）水平。采用SPSS 17.0软件包对数据进行统计学分析。结果: 与未转染的空白对照组和阴性空病毒载体转染组相比,PRRX1过表达组的PRRX1表达量升高,自噬小体减少,自噬标志物水平降低（P<0.05）。结论: PRRX1对SACC细胞的自噬有抑制作用。

关键词: 配对相关同源框1, 唾液腺腺样囊性癌, 自噬

Abstract: PURPOSE: To investigate the effect of paired related homeobox1 (PRRX1) on autophagy of salivary adenoidcystic carcinoma (SACC) cells. METHODS: PRRX1-overexpressed lentiviral vectors and their negative control lentiviral vectors were used to transfect SACC cells. The transfection effect was detected by Western blot. Autophagosome was observed under transmission electron microscopy(TEM). The level of autophagy markers (LC3-II/Beclin1) was detected by immunofluorescence and Western blot. Statistical analysis was performed with SPSS 17.0 software package. RESULTS: PRRX1 protein level increased, autophagosome number decreased, and autophagy marker level decreased in the PRRX1 overexpressed group, compared to the control (P<0.05). CONCLUSIONS: PRRX1 inhibits the autophagy of SACC cells.

Key words: Paired related homeobox1, Salivary adenoid cystic carcinoma, Autophagy

中图分类号:

R739.8

高洁, 姜雅萍, 马康傑, 赵保东, 邓婧. PRRX1对唾液腺腺样囊性癌细胞自噬的影响[J]. 上海口腔医学, 2021, 30(5): 477-480.

GAO Jie, JIANG Ya-ping, MA Kang-jie, ZHAO Bao-dong, DENG Jing. Effect of PRRX1 on autophagy of salivary adenoid cystic carcinoma cells[J]. Shanghai Journal of Stomatology, 2021, 30(5): 477-480.

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PRRX1对唾液腺腺样囊性癌细胞自噬的影响

Effect of PRRX1 on autophagy of salivary adenoid cystic carcinoma cells

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