NPP/C-HA/rhBMP-2复合支架修复兔股骨缺损的实验研究

doi:10.19439/j.sjos.2021.04.002

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (4): 344-349.doi: 10.19439/j.sjos.2021.04.002

NPP/C-HA/rhBMP-2复合支架修复兔股骨缺损的实验研究

杨艳兰¹, 徐普¹, 张文柏¹, 陈灼庚¹, 李晓妮²

1.中南大学湘雅医学院附属海口医院口腔中心·海南省口腔医学中心口腔种植科,海南海口 570208;
2.青岛市口腔医院口腔种植科,山东青岛 266000

收稿日期:2019-11-19 修回日期:2020-03-17 出版日期:2021-08-25 发布日期:2021-09-23
通讯作者: 徐普,E-mail:hnxupu@163.com
作者简介:杨艳兰(1982-),女,硕士,主治医师,E-mail:1094645212@qq.com
基金资助:
海南省重点研发计划项目(ZDYF2016018)

Experimental study of repairing rabbit femur defect with NPP/C-HA/ rhBMP-2

YANG Yan-lan¹, XU Pu¹, ZHANG Wen-bo¹, CHEN Zhuo-geng¹, LI Xiao-ni²

1. Department of Oral Implant, Affiliate Haikou Hospital of Xiangya Medical School, Center South University·Hainan Provincial Stomatology Center. Haikou 570208, Hainan Province;
2. Department of Oral Implant, Qingdao Stomatological Hospital. Qingdao 266000, Shandong Province, China

Received:2019-11-19 Revised:2020-03-17 Online:2021-08-25 Published:2021-09-23

摘要/Abstract

摘要： 目的: 探讨NPP/C-HA/rhBMP-2人工骨在实验兔体内的成骨效果。方法: 在兔股骨远端做直径7 mm、深10 mm的骨缺损模型,根据缺损形状,制作含有rhBMP-2的NPP/C-HA人工骨,缺损处不植入材料作为空白组,植入NPP/C-HA人工骨为对照组,植入NPP/C-HA/rhBMP-2人工骨为实验组。第4、8、12周时,通过锥形束CT（CBCT）、H-E染色及Masson染色,观察各组成骨效应;检测各组血清中碱性磷酸酶（ALP）活性及组织中OCN阳性染色,确定缺损处成骨分化及骨成熟程度。采用SPSS 18.0软件包对数据进行统计学分析。结果: 大体标本显示,实验组12周时缺损处修复完全,未见炎症、排斥等免疫反应。CBCT显示,12周时,对照组从骨缺损边缘到中心处骨密度增高,但边界仍在;加入rhBMP-2后的实验组效果更加明显,新生骨与周围宿主骨基本一致,边界消失。第8、12周时,实验组HU值显著高于对照组及空白组（P<0.05）。H-E染色及Masson染色显示,第8、12周时,实验组较对照组及空白组有明显的新生骨形成。第8周时,各组血清ALP活性升高,实验组ALP水平显著高于其他组（P<0.05）。OCN免疫组织化学IHC阳性染色显示,实验组较对照组、空白组更明显（P<0.05）。结论: NPP/C-HA/rhBMP-2具有良好的组织相容性、骨诱导性、骨传导性和成骨性,有望成为骨缺损修复良好材料。

关键词: 纳米级珍珠粉, 人工骨, 重组人骨形态蛋白-2, 骨修复

Abstract: PURPOSE: To investigate the osteogenic effect of nano-grade pearl powder(NPP)/chitosan-hyaluronic acid (C-HA)/recombinant human bone morphology protein-2 (rhBMP-2) artificial bone. METHODS: A bone defect model with a diameter of 7 mm and a height of 10 mm was made at the distal end of the femur. NPP/C-HA stent containing rhBMP-2 was prepared according to the shape of the defect. No material was implanted in the defect as blank group. NPP/C-HA was used as the control group, NPP/C-HA/rhBMP-2 was implanted into the experimental group. At 4 weeks, 8 weeks, and 12 weeks, the bone effects of each component were detected by cone-beam CT(CBCT), H-E and Masson staining. Serum ALP activity and OCN in tissues to determine the osteogenic differentiation and osteogenesis maturity were detected. SPSS 18.0 software package was used for statistical analysis. RESULTS: At 12 weeks, the defect was completely repaired in the experimental group. No immunological side effects such as inflammation and rejection were observed. At 8 and 12 weeks, CBCT showed that the experimental group had a higher CT value (Hounsfield units, HU) compared with the control group and the blank group(P<0.05). H-E and Masson staining showed that the experimental group had obvious new bone formation compared with the control group and the blank group at 8 weeks and 12 weeks, and ALP activity of the experimental group was significantly different from the control group and the blank group at 8 weeks. OCN immunohistochemical scoring of the experimental group was significantly different from the control group and the blank group(P<0.05). CONCLUSIONS: NPP/C-HA/rhBMP-2 has good tissue fusion, osteoinductivity, osteoconductivity and osteogenicity, which is expected to provide more effective treatment for bone repair.

Key words: Nano pearl powder, Artificial bone, Recombinant human bone morphology protein-2, Bone repair

中图分类号:

R783.1

杨艳兰, 徐普, 张文柏, 陈灼庚, 李晓妮. NPP/C-HA/rhBMP-2复合支架修复兔股骨缺损的实验研究[J]. 上海口腔医学, 2021, 30(4): 344-349.

YANG Yan-lan, XU Pu, ZHANG Wen-bo, CHEN Zhuo-geng, LI Xiao-ni. Experimental study of repairing rabbit femur defect with NPP/C-HA/ rhBMP-2[J]. Shanghai Journal of Stomatology, 2021, 30(4): 344-349.

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NPP/C-HA/rhBMP-2复合支架修复兔股骨缺损的实验研究

Experimental study of repairing rabbit femur defect with NPP/C-HA/ rhBMP-2

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