牙髓卟啉单胞菌脂多糖对小鼠成骨细胞表达MIP-1α的影响

doi:10.19439/j.sjos.2017.03.006

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (3): 263-267.doi: 10.19439/j.sjos.2017.03.006

牙髓卟啉单胞菌脂多糖对小鼠成骨细胞表达MIP-1α的影响

于雅琼^{1, 2, 3}, 李晓琳^{1, 2, 3}, 仇丽鸿^{1, 2, 3}, 郭佳杰^{1, 2, 3}, 杨谛^{1, 2, 3}, 郭艳^{3, 4}

1.中国医科大学口腔医学院牙体牙髓病科,辽宁沈阳 110002；
2.辽宁省口腔医学研究所牙体牙髓病学研究室,辽宁沈阳 110002；
3.辽宁省口腔疾病转化医学研究中心,辽宁沈阳 110002；
4.中国医科大学口腔医学院中心实验室,辽宁沈阳 110002

收稿日期:2016-10-10 修回日期:2016-12-12 出版日期:2017-06-25 发布日期:2017-07-05
通讯作者: 仇丽鸿,E-mail：drqlh@yahoo.com
作者简介:于雅琼（1987-）,女,医师,讲师,E-mail：yuyaqiong1987@126.com
基金资助:
沈阳市科技计划项目（F15-199-1-56）

Effects of lipopolysaccharides from Porphyromonas endodontalis on the expression of MIP-1α in mouse osteoblasts

YU Ya-qiong^{1, 2, 3}, LI Xiao-lin^{1, 2, 3}, QIU Li-hong^{1, 2, 3}, GUO Jia-jie^{1, 2, 3}, YANG Di^{1, 2, 3}, GUO Yan^{3, 4}

1.Department of Endodontics,School of Stomatology,China Medical University. Shenyang 110002;
2. Lab of Endodontic,Liaoning Institute of Dental Research.Shenyang 110002;
3.Liaoning Provincial Research Center of Translational Oral Medicine.Shenyang 110002;
4.Central Laboratory,School of Stomatology,China Medical University.Shenyang 110002,Liaoning Province,China

Received:2016-10-10 Revised:2016-12-12 Online:2017-06-25 Published:2017-07-05

摘要/Abstract

摘要： 目的探讨牙髓卟啉单胞菌（Porphyromonas endodontalis, P.e）脂多糖（lipopolysaccharide,LPS）对成骨细胞产生巨噬细胞炎性蛋白1α （macrophageinflammatoryprotein-1α,MIP-1α） mRNA和蛋白分泌的影响,以及姜黄素对此过程产生的抑制作用。方法以20 mg/L P.e-LPS作用细胞不同时间(0~48 h)后,采用实时反转录聚合酶链反应(real-time reverse transcription-polymerase chain reaction,real-time RT-PCR)和酶联免疫吸附试验（enzyme linked immunosorbent assay,ELISA）检测MIP-1α mRNA和蛋白的表达。以同样方法检测姜黄素对20 mg/L P.e-LPS 刺激MC3T3-El细胞后MIP-1α mRNA和蛋白表达的影响。采用SPSS 13.0软件包对结果进行单因素方差分析和Dunnett t检验。结果在观察时间内（0~48 h）,20 mg/L P.e-LPS作用MC3T3-El细胞后,MIP-1α mRNA 的表达和蛋白的分泌具有时间依赖性。10 mol/L 姜黄素预处理细胞1 h,可以降低P.e-LPS诱导的MIP-1α mRNA和蛋白的表达水平。结论P.e-LPS 可以诱导成骨细胞表达和分泌MIP-1α,姜黄素对此过程发挥明显的抑制作用。

关键词: 牙髓卟啉单胞菌, 脂多糖, 成骨细胞, 巨噬细胞炎性蛋白1α, 姜黄素

Abstract: PURPOSE: To investigate the effects of lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(P.e) on the expression of macrophageinflammatoryprotein-1α (MIP-1α) mRNA and protein levels in MC3T3-E1 cells and the influence of curcumin in the process. METHODS: MC3T3-E1 cells were treated with 20 mg/L P.e-LPS for different times (0-48 h). The expression of MIP-1α mRNA and protein was detected by real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) and enzyme linked immunosorbent assay(ELISA). MC3T3-E1 cells were pretreated with inhibitor of (curcumin) for 1 h, and then treated with 20 mg/L P.e-LPS. The expression of MIP-1α was also detected by real-time RT-PCR and ELISA.Statistical analysis was performed using one-way ANOVA and Dunnett's t test with SPSS 13.0 software package. RESULTS: In the observation time (0~48 h), the impact of 20 P.e-LPS mg/L on induction of MIP-1α in MC3T3-El cells exhibited a time-dependent manner. The expression of MIP-1α mRNA and protein decreased significantly after pretreatment with 10 μmol/L curcumin for 1 h. CONCLUSIONS: The results suggest that P.e-LPS may mediate MIP-1α expression in MC3T3-E1 cells, and curcumin has a significant inhibitory effect on this process.

Key words: Porphyromonas endodontalis, Lipopolysaeeharides, Osteoblast, Macrophageinflammatoryprotein-1α, Curcumin

中图分类号:

R780.2

于雅琼, 李晓琳, 仇丽鸿, 郭佳杰, 杨谛, 郭艳. 牙髓卟啉单胞菌脂多糖对小鼠成骨细胞表达MIP-1α的影响[J]. 上海口腔医学, 2017, 26(3): 263-267.

YU Ya-qiong, LI Xiao-lin, QIU Li-hong, GUO Jia-jie, YANG Di, GUO Yan. Effects of lipopolysaccharides from Porphyromonas endodontalis on the expression of MIP-1α in mouse osteoblasts[J]. Shanghai Journal of Stomatology, 2017, 26(3): 263-267.

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牙髓卟啉单胞菌脂多糖对小鼠成骨细胞表达MIP-1α的影响

Effects of lipopolysaccharides from Porphyromonas endodontalis on the expression of MIP-1α in mouse osteoblasts

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