PUMA在唾液腺腺样囊性癌中的表达及临床意义

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (3): 314-316.

PUMA在唾液腺腺样囊性癌中的表达及临床意义

雷印涛¹, 李俊福¹, 姜娟¹, 李晓光², 王旭霞³, 陈岱韻¹

1.泰山医学院附属医院口腔科,山东泰安 271000;
2.泰安市中心医院口腔科,山东泰安 271000;
3.山东大学口腔医学院,山东济南 250012

收稿日期:2015-08-07 修回日期:2015-11-06 出版日期:2016-06-25 发布日期:2016-07-22
通讯作者: 陈岱韻,E-mail：daiyunchen@126.com E-mail:yintaolei@163.com
作者简介:雷印涛（1969-）,男,学士,副主任医师
基金资助:
泰安市科技发展计划（201340629）

Expression and clinical significance of PUMA gene in salivary adenoid cystic carcinoma

LEI Yin-tao¹, LI Jun-fu¹, JIANG Juan¹, LI Xiao-guang², WANG Xu-xia³, CHEN Dai-yun¹

1. Department of Stomatology, Affiliated Hospital of Taishan Medical University. Tai'an 271000;
2. Department of Stomatology, Tai'an City Central Hospital. Tai'an 271000;
3. School of Stomatology Shandong University. Jinan 250012, Shandong Province, China

Received:2015-08-07 Revised:2015-11-06 Online:2016-06-25 Published:2016-07-22

摘要/Abstract

摘要： 目的研究p53上调凋亡调控因子（p53 up-regulated modulator of apoptosis,PUMA）在唾液腺腺样囊性癌（salivary cystic carcinoma, SACC）癌组织及癌旁组织中的表达,探讨PUMA在SACC发生、发展中的作用。方法利用实时定量PCR（RT-PCR）和Western 免疫印迹法分别检测27例SACC癌旁组织和27例SACC癌组织中PUMA基因和PUMA蛋白的表达。采用 SPSS19.0 软件包对数据进行统计学分析。结果以SACC癌旁组织作为对照样本,其PUMA基因表达相对定量为1,SACC癌组织中PUMA基因表达相对定量为0.57±0.17。PUMA蛋白在SACC癌旁组织和癌组织中表达的相对量分别为0.94±0.12和0.36±0.12。SACC癌组织中PUMA基因和PUMA蛋白的表达显著低于SACC癌旁组织(P<0.05)。结论 PUMA基因在SACC中的表达下调,与SACC的发生呈负相关。

关键词: p53上调凋亡调控因子, 唾液腺腺样囊性癌, 实时定量PCR

Abstract: PURPOSE: To explore the expression of p53 up-regulated modulator of apoptosis (PUMA) gene in salivary adenoid cystic carcinoma (SACC) and its clinical significance. METHODS: The expressions of PUMA gene and protein were detected in 27 SACC tumor-adjacent tissues (group A) and 27 SACC tumor tissues (group B) with real-time quantitative PCR and Western blot. Statistical analysis was performed using SPSS 19.0 software package. RESULTS: The expression of PUMA gene in groups A as calibrator was 1,and expression of PUMA gene in group B was 0.57±0.17. The expression of PUMA protein in groups A and B were 0.94±0.12 and 0.36±0.12,respectively. The expressions of PUMA gene and protein were significantly lower in group B than in group A (P<0.05). CONCLUSIONS: The expression of puma gene in SACC tissues is down-regulated, which is negatively correlated with the development of SACC.

Key words: p53 up-regulated modulator of apoptosis, Salivary adenoid cystic carcinoma, Real-time quantitative PCR

中图分类号:

R739.8

雷印涛, 李俊福, 姜娟, 李晓光, 王旭霞, 陈岱韻. PUMA在唾液腺腺样囊性癌中的表达及临床意义[J]. 上海口腔医学, 2016, 25(3): 314-316.

LEI Yin-tao, LI Jun-fu, JIANG Juan, LI Xiao-guang, WANG Xu-xia, CHEN Dai-yun. Expression and clinical significance of PUMA gene in salivary adenoid cystic carcinoma[J]. Shanghai Journal of Stomatology, 2016, 25(3): 314-316.

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