桂皮醛对成骨细胞成骨功能及BMP-2 mRNA表达的影响

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (3): 271-274.

桂皮醛对成骨细胞成骨功能及BMP-2 mRNA表达的影响

郝文秀, 张云涛, 张雅杰

滨州医学院附属医院口腔修复科,山东滨州 256603

收稿日期:2015-08-04 修回日期:2015-11-25 出版日期:2016-06-25 发布日期:2016-07-22
通讯作者: 张云涛,E-mail：zytao999@163.com E-mail:haowenxiu89@163.com
作者简介:郝文秀（1989-）,女,硕士研究生
基金资助:
山东省科学技术发展计划（2011Y121008）

Effects of cinnamaldehyde on osteogenic function and BMP-2 mRNA expression of mice osteoblast

HAO Wen-xiu, ZHANG Yun-tao, ZHANG Ya-jie

Department of Prosthodontics, Affiliated Hospital to Binzhou Medical University. Binzhou 256603, Shandong Province, China

Received:2015-08-04 Revised:2015-11-25 Online:2016-06-25 Published:2016-07-22

摘要/Abstract

摘要： 目的探讨不同浓度桂皮醛液(cinnamaldehyde, CA)对成骨细胞成骨功能及BMP-2表达的影响。方法以0（对照组）、10、20、40、80、160、320 μg/mL的CA作用于小鼠颅顶前骨细胞亚克隆14（MC3T3-E1 subclone 14, MC3T3）,MTT法检测在CA作用1、3、5 d后的增殖情况,分别在3、5、7 d检测碱性磷酸酶( alkaline phosphatase, ALP)活性,以观察6种浓度的CA对成骨细胞成骨功能的影响;荧光实时定量PCR(quantitative real-time PCR,RT-qPCR)检测BMP-2 mRNA在低浓度组(10、20 μg/ml)CA作用1、3、5 d后的表达。采用SPSS 17.0 软件包对数据进行统计学分析。结果 MTT显示,10、20 μg/mL的CA作用于MC3T3后,与空白对照组相比,吸光度值稍有下降但差异不显著 (P>0.05),ALP活性显著提高;RT-qPCR结果显示,10、20 μg/mL的CA促进BMP-2 mRNA的表达(P<0.05),40、80、160、320 μg/mL的CA可抑制成骨细胞增殖、ALP活性及BMP-2 mRNA的表达（P<0.05）,随着浓度升高,抑制作用更明显。结论低浓度CA可提高成骨细胞的ALP活性,促进成骨和BMP-2 mRNA表达,其机制可能是通过上调BMP-2基因表达。

关键词: 桂皮醛, 成骨细胞, 碱性磷酸酶, BMP-2

Abstract: PURPOSE: To explore the effect of cinnamaldehyde(CA) on osteogenic function and BMP-2 expression of mice osteoblast. METHODS: MC3T3-E1 sub-clone 14 cells were cultured in the presence of CA at 0(control group), 10, 20, 40, 80, 160, 320 μg/mL. The cell proliferation rate was measured by MTT assay 1, 3, 5 d after exposure. Activity of alkaline phosphatase (ALP) was detected at 3, 5, 7 d to observe the effect of 6 concentrations of CA on osteoblast function. The mRNA expression of BMP-2 was quantified by real-time PCR(RT-qPCR) 1, 3, 5 d after exposure to (10,20 μg/mL) CA. The data were analyzed by SPSS 17 software package. RESULTS: MTT assay showed that compared with the control group, when 10, 20 μg/mL of CA acted on MC3T3, the absorbance value decreased slightly but the difference was not statistically significant (P>0.05), while ALP activity increased significantly. CA increased the mRNA expression of BMP-2 at the concentration of 10, 20 μg/mL (P<0.05),decreased the osteoblast proliferation, ALP activity and the mRNA expression of BMP-2 at the concentration of 40, 80, 160, 320 μg/mL (P<0.05); as the concentration of CA increased, the effects became more significant. CONCLUSIONS: CA(10, 20 μg/mL) exerted positive effects on osteogenic function, increased the ALP activity and the mRNA expression of BMP-2. It is suggested that the activity of osteoblast differentiation and osteogenic function may be related to the expression of BMP-2.

Key words: Cinnamaldehyde, Osteoblast, ALP, BMP-2

中图分类号:

R739.8

郝文秀, 张云涛, 张雅杰. 桂皮醛对成骨细胞成骨功能及BMP-2 mRNA表达的影响[J]. 上海口腔医学, 2016, 25(3): 271-274.

HAO Wen-xiu, ZHANG Yun-tao, ZHANG Ya-jie. Effects of cinnamaldehyde on osteogenic function and BMP-2 mRNA expression of mice osteoblast[J]. Shanghai Journal of Stomatology, 2016, 25(3): 271-274.

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