牙周非手术治疗对龈沟液MMP-8及其抑制剂TIMP-1水平的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (2): 229-232.

牙周非手术治疗对龈沟液MMP-8及其抑制剂TIMP-1水平的影响

王丽琴, 冯坤, 孙利

上海市口腔病防治院口腔内科, 上海 200031

收稿日期:2013-09-27 出版日期:2014-04-20 发布日期:2014-05-21
作者简介:王丽琴(1977-), 女, 硕士, 主治医师王丽琴, E-mail:caiju_donglixia@163.com

The influences of non-surgical periodontal treatment on gingival crevicular fluid levels of MMP-8 and tissue inhibitor of TIMP-1

WANG Li-qin, FENG Kun, SUN Li

Department of Oral Medicine, Shanghai Stomatological Disease Center. Shanghai 200031, China

Received:2013-09-27 Online:2014-04-20 Published:2014-05-21

摘要/Abstract

摘要： 目的: 比较慢性牙周炎患者非手术治疗前、后龈沟液MMP-8、TIMP-1水平的变化。方法: 选择慢性中重度牙周炎30例, 治疗前、后临床检查牙龈出血指数(SBI)、菌斑指数(PLI)、探诊深度(PD), 采集龈沟液, 采用双抗体夹心ABC-ELISA法检测MMP-8、TIMP-1的含量。采用SSPS 19.0软件包对数据进行配对样本t检验和多元相关分析。结果: 治疗后MMP-8、TIMP-1水平显著下降, MMP-8/TIMP-1 比率治疗前、后无显著差异。多元相关分析各变量间相关性, 发现MMP-8和龈沟液重量呈正相关关系。结论: 牙周非手术治疗能显著降低龈沟液MMP-8、TIMP-1水平, 从而减轻牙周组织损伤。

关键词: 慢性牙周炎, 龈沟液, MMP-8, TIMP-1

Abstract: PURPOSE: To compare the levels of MMP-8 and TIMP-1 in the gingival crevicular fluid (GCF)of chronic periodontitis(CP) patients before and after non-surgical periodontal therapy. METHODS: Sulcus bleeding index (SBI), plaque index (PLI) and probing depth (PD) were detected before and after therapy. GCF was collected from CP patients before and after therapy. MMP-8 and TIMP-1 were determined by enzyme-linked immunoabsorbent assay. The data was analyzed using paired-sample t test and multi-correlation analysis with SSPS 19.0 software package. RESULTS: The levels of MMP-8 and TIMP-1 decreased after therapy, and there was no significant difference in the MMP-8/TIMP-1 ratio. Correction analysis showed that a significant correction was found between MMP-8 and GCF weight. CONCLUSIONS: MMP-8 and TIMP-1 decrease after non-surgical periodontal therapy.

Key words: Chronic periodontitis, GCF, MMP-8, TIMP-1

中图分类号:

R781.4

王丽琴, 冯坤, 孙利. 牙周非手术治疗对龈沟液MMP-8及其抑制剂TIMP-1水平的影响[J]. 上海口腔医学, 2014, 23(2): 229-232.

WANG Li-qin, FENG Kun, SUN Li. The influences of non-surgical periodontal treatment on gingival crevicular fluid levels of MMP-8 and tissue inhibitor of TIMP-1[J]. Shanghai Journal of Stomatology, 2014, 23(2): 229-232.

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