上海口腔医学

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (5): 523-527.

• 基础研究 • 上一篇    下一篇

胰岛素对高糖环境下大鼠颌骨骨髓基质细胞成骨分化过程中peroxiredoxin-6表达的影响

王俊成1 , 李颖2 ,王东胜1 ,鄂玲玲1 , 刘洪臣1   

  1. (1.解放军总医院 口腔医学研究所，北京 100853；2.空军总医院 口腔科，北京 100142)
  • 收稿日期:2013-02-15 修回日期:2013-03-21 出版日期:2013-10-10 发布日期:2013-10-10
  • 通讯作者: 刘洪臣, Tel:010-66936254，Fax：010-66937949 ,E-mail:liuhongchen301@hotmail.com
  • 作者简介:王俊成(1979-)，男，主治医师，博士研究生，E-mail:wbhwjc527@126.com

Effect of insulin on peroxiredoxin-6 in the osteogenic differentiation of rat’s mandiular bone marrow stromal cells in high glucose

WANG Jun-cheng1, LI Ying2, WANG Dong-sheng1, E Ling-ling1, LIU Hong-chen1   

  1. 1.Institute of Stomatology, General Hospital of Chinese PLA. Beijing 100853; 2. Department of Stomatology, General Hospital of the Air Force of Chinese PLA. Beijing 100142, China
  • Received:2013-02-15 Revised:2013-03-21 Online:2013-10-10 Published:2013-10-10

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摘要： 目的：探讨胰岛素对高糖环境下大鼠颌骨骨髓基质细胞(rat bone marrow stromal cells, rBMSCs)成骨分化过程中peroxiredoxin-6表达的影响。方法：从Wistar大鼠下颌骨中分离培养骨髓基质细胞，分别于不同糖浓度(5.5、25、45 mmol/L)及胰岛素(0、10-5 mol/L)的干预下成骨诱导(5.5 mmol/L组、5.5 mmol/L +Ins组、25 mmol/L组、25 mmol/L+Ins组、45 mmol/L组、45 mmol/L+Ins组)。成骨诱导21 d后，进行茜素红染色，矿化结节定量分析。成骨诱导1、3、7、14、21 d后， 用ELISA试剂盒检测peroxiredoxin-6蛋白的表达。采用SPSS15.0软件包对数据进行统计学分析。结果：45 mmol/L高糖环境显著抑制rBMSCss的矿化，胰岛素可改善高糖对rBMSCs矿化的抑制。45 mmol/L组peroxiredoxin-6的表达较5.5 mmol/L组和25 mmol/L组均显著下降。矿化诱导第21天，各组peroxiredoxin-6蛋白的表达均达到最高峰。在25 mmol/L和45 mmol/L的糖浓度下，10-5 mol/L的胰岛素可显著上调rBMSCs peroxiredoxin-6蛋白的表达。结论：高糖环境抑制peroxiredoxin-6蛋白的表达，胰岛素可改善高糖环境对peroxiredoxin-6蛋白在大鼠颌骨骨髓基质细胞成骨分化过程中表达的影响。Peroxiredoxin-6蛋白在大鼠颌骨骨髓基质细胞成骨分化后期表达显著。

关键词: 骨髓基质细胞, 高糖环境, Peroxiredoxin-6蛋白, 成骨分化

Abstract: PURPOSE: To explore the effect of insulin on the expression of peroxiredoxin-6 in osteogenic differentiation of rat’s mandibular bone marrow stromal cells(rBMSCs) in high glucose. METHODS: Bone marrow stromal cells were obtained from the mandible of Wistar rats and stimulated in three glucose concentrations mineral medium(5.5 mmol/L, 25 mmol/L and 45 mmol/L) with or without insulin(10-5mol/L) for 1, 3, 7, 14, and 21 days. The expression of prohibitin was quantified via enzyme-linked immuno sorbent assays (ELISA). The mineralization nodules were assessed at day 21 by alizarine red staining. Statistical analysis was performed using SPSS 15.0 soft ware package. RESULTS: High glucose of 45 mmol/L inhibited mineralization of rBMSCs and insulin can improve the mineralization in high glucose. The expression of peroxiredoxin-6 in 45 mmol/L group decreased significantly compared with 5.5 mmol/L group and 25 mmol/L group. The expression of peroxiredoxin-6 in each group achieved maximum at day 21. Insulin (10-5 mol/L) increased the expression of peroxiredoxin-6 in 25 mmol/L group and 45 mmol/L group in osteogenic differentiation of rBMSCs. CONCLUSIONS: High glucose inhibits the expression of peroxiredoxin-6 in osteogenic differentiation of rBMSCs, while insulin upregulates the expression of peroxiredoxin-6 in rBMSCs. Peroxiredoxin-6 may play an important part in later stage in osteogenic differentiation of rBMSCs.

Key words: Bone marrow stromal cells, High glucose, Peroxiredoxin-6, Osteogenic differentiation

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