上海口腔医学

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (4): 414-417.

• 基础研究 • 上一篇    下一篇

复合树脂、玻璃离子对人巨噬细胞的增殖活性和功能的影响

龚瑜1，杨荣强2，杨红丽1   

  1. (1.昆明医科大学附属口腔医院 急诊科，云南 昆明 650031；2.昆明医科大学口腔医学研究所，云南 昆明 650031)
  • 收稿日期:2013-01-08 修回日期:2003-03-12 出版日期:2013-08-10 发布日期:2013-08-10
  • 通讯作者: 龚瑜，Tel:0871-68337123,E-mail:318731210@qq.com
  • 作者简介:龚瑜(1970-)，女，硕士，主治医师

Effects of composite resin and glass-ionomer cements on proliferation and functional activity of human macrophages

GONG Yu1, YANG Rong-qiang2, YANG Hong-li1   

  1. 1.Department of Emergency, Affiliated Stomatological Hospital of Kunming Medical University. Kunming 650031; 2. Institute of Stomatology of Kunming Medical University. Kunming 650031, Yunnan Province, China
  • Received:2013-01-08 Revised:2003-03-12 Online:2013-08-10 Published:2013-08-10

PDF (PC)

256

被引次数

1

摘要： 目的：研究常用复合树脂、玻璃离子对体外培养的人单核-巨噬细胞增殖活性和功能的影响。方法：体外诱导培养人的单核血细胞株THP-1，获得巨噬细胞，MTT 法测定2种常用复合树脂材料Filtek Z350(3M)、Filtek P60(3M)和玻璃离子材料的24 h浸渍液对体外培养的人单核-巨噬细胞增殖活性的影响，ELISA试验检测各组材料浸渍液对细胞分泌 IL-1细胞因子活性的影响。采用SPSS 17. 0 软件包对数据进行统计学分析。结果：与对照组相比,2种复合树脂材料浸渍培养液对体外培养的人单核-巨噬细胞的增殖活性有促进作用，与对照组相比差异有显著性(P<0.05) ;检测各材料对巨噬细胞分泌IL-1的影响，2种复合树脂材料与对照组相比，差异没有显著性(P>0.05)；玻璃离子与对照组相比，差异有显著性(P<0.05)。结论：复合树脂对人巨噬细胞的增殖和功能活性有显著影响，可能与复合树脂充填产生牙本质敏感症状有关。玻璃离子对人巨噬细胞的增殖活性无明显影响，但可能会促进炎症发展。

关键词: 复合树脂, 巨噬细胞, 牙本质敏感症

Abstract: PURPOSE: To study the effects of composite resin and glass-ionomer cements on cell proliferation and function of human macrophages in vitro. METHODS: Macrophages were differentiated from THP-1 cells after treatment with phorbol ester and used as the model of inflammatory cells, which were treated by specimens from glass-ionomer cements(GC), composite resin Filtek Z350(3M)and Filtek P60(3M) on culture medium for 24 hours. The cell proliferation of the tooth-colored restorative materials on human macrophages in vitro was evaluated by MTT color imetric assay, and determined for IL-1 content in these material specimens by ELISA.All statistical analyses were performed using the SPSS 17.0 software package. RESULTS: Compared with control group, composite resin Filtek Z350(3M) and Filtek P60(3M) significantly enhanced the proliferation of human macrophages (P<0.05), while Glass-ionomer had little effect on the proliferation of human macrophages (P>0.05). Glass-ionomer could promote macrophages to secrete IL-1β and the difference was statistically significant(P<0.05). The composite resin could not cause release of IL- 1β from macrophages (P>0.05). CONCLUSIONS: Composite resin enhances proliferation and function of human macrophages. The effect may be associated with hypersensitivity of dentin. Glass-ionomer cement has little effect on proliferation of macrophages, but may lead to progress of inflammation.

Key words: Compositresin, Macrophages, Dentin hypersensitivity

中图分类号: