钛片表面粗糙度和氧化膜对成骨细胞增殖和分化的影响

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (3): 282-286.

钛片表面粗糙度和氧化膜对成骨细胞增殖和分化的影响

周屹立¹，丁仲鹃¹，唐玲²

(1.昆明医学院口腔医学院口腔修复学教研室，云南昆明 650031；2.昆明市第一人民医院口腔科，云南昆明 650032)

收稿日期:2013-02-08 修回日期:2013-04-10 出版日期:2013-06-10 发布日期:2013-06-10
通讯作者: 丁仲鹃，Tel:0871-5330099，E-mail: dingzhongjuan@163.com
作者简介:周屹立(1972-)，男，硕士，主治医师，E-mail: smcocr@yahoo.cn

Effect of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts

ZHOU Yi-li¹, DING Zhong-juan¹, TANG Ling²

1.Department of Prosthodontics, College of Stomatology, Kunming Medical University. Kunming 650031; 2.Department of Prosthodontics, The First People’s Hospital of Kunming. Kunming 650032, Yunnan Province, China

Received:2013-02-08 Revised:2013-04-10 Online:2013-06-10 Published:2013-06-10

摘要/Abstract

摘要： 目的：研究钛片表面粗糙度和氧化膜对成骨细胞增殖和分化的影响，为种植体表面处理提供理论依据。方法：采用粒度分别为108～130 μm(S1)、216～301 μm(S2)和356～411 μm(S3)的二氧化钛颗粒对纯钛钛片表面进行喷砂处理，钛浆喷涂(titanium-sprayed plasma, TPS)表面处理组由Straumman 公司提供，600目砂纸打磨组(S0)作为对照组，在钛片表面进行成骨细胞培养。采用表面轮廓测量仪测量其表面粗糙度，电子探针(electron microprobe)测定钛片表面氧化膜结构。分别在1、3、5及7 d时，采用四锉盐比色(MTT)法检测不同处理表面对成骨细胞增殖(OD值)的影响；通过碱性磷酸酶活性(ALP)及骨钙素分泌(OC)检测比较不同处理的表面对成骨细胞分化的影响。采用SPSS12.0软件包对数据进行单因素方差分析。结果：S0 、S1 、S2 、S3 和TPS组表面粗糙度由0.372 μm至5.239 μm递增；喷砂组钛片表面氧化膜结构完整、连续；粗糙表面比光滑表面更利于成骨细胞增殖和分化。喷砂表面粗糙度越高，越利于成骨细胞增殖和分化。S3组成骨细胞增殖和分化优于TPS组。结论：表面粗糙度较高的喷砂表面，更利于成骨细胞增殖和分化。

关键词: 成骨细胞, 喷砂处理, 钛浆喷涂, 增殖, 分化

Abstract: PURPOSE: To study the effects of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts. METHODS: Osteoblasts were cultured on 5 commercially pure titanium (cp Ti) substrates of ground (S0), blasted with 108～130 μm(S1), 216～301 μm(S2), 356～411 μm (S3) TiO2 particles and titanium-sprayed plasma(TPS) surfaces. Surfaces prepared by hand grinding with SiC paper of 600 grits served as control (S0). Electron microprobe was used to evaluate the TiO2 film structure of the 5 titanium surfaces. For proliferation and differentiation measurement, osteoblasts were cultured for 1, 3, 5 and 7 days，evaluated by MTT Assay, ALP activity and OC level. SPSS12.0 software package was used for one-way ANOVA. RESULTS: The number of cells was the highest on S3 and TPS surfaces after 1 day culture. The same results were observed after 5 days. On day 3 and 7, S3 surface was the highest(P<0.05). The number of cells on all experimental groups were higher than S0 surfaces at each time point(P<0.05). Increase of ALP activity were detected on S0, S1 and S2 surfaces after 1, 3, 5 days. However, there was no difference between S3 and TPS surfaces(P>0.05). After 7 days, ALP activity increased significantly on TPS surface than on S1 or S2 surfaces. ALP activity on S3 surfaces was the highest(P<0.05). The ALP activity on all experimental groups were higher than S0 surfaces at each time point(P<0.05). An increase in OC production was detected on S0, S1, S2 and S3 surfaces after 1, 3, 5 days. The highest OC production was on S3 surface was on day 7(P<0.05). CONCLUSIONS: It was concluded that for TiO2 blasted surfaces, Ra ranging from 1.260 μm to 3.530 μm would optimize proliferation and differentiation of osteoblasts. Blasting was an effective treatment method for osteointegration in vitro.

Key words: Osteoblast, Sandblasted, Titanium-sprayed-plasma, Proliferation, Differentiation

中图分类号:

R783.6

周屹立, 丁仲鹃, 唐玲. 钛片表面粗糙度和氧化膜对成骨细胞增殖和分化的影响[J]. 上海口腔医学, 2013, 22(3): 282-286.

ZHOU Yi-li, DING Zhong-juan, TANG Ling. Effect of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts[J]. Shanghai Journal of Stomatology, 2013, 22(3): 282-286.

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