A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells

Shanghai Journal of Stomatology ›› 2013, Vol. 22 ›› Issue (6): 628-633.

• Basic Scientific Study • Previous Articles Next Articles

A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells

LI Jun-liang¹, WANG Wen-mei¹, GE Jiu-yu¹, JI Jun², WANG Tian-cong²

1.Department of Endodontics, 2. Department of Orthodontics, Nanjing Stomatological Hospital, Central Laboratory of Stomatological School of Nanjing University. Nanjing 210008, Jiangsu Province, China

Received:2013-08-22 Revised:2013-09-23 Online:2013-04-12 Published:2013-04-12
Supported by:
Supported by Medical Leading Talents and Innovation Team Project of Jiangsu Province (LJ201110) and Natural Science Foundation of Jiangsu Province (SBK201340904).

Abstract

Abstract: PURPOSE: To compare the cytotoxicity of 5 different dental bonding agents to human periodontal ligament cells(HPDLCs). METHODS: Primary cultured HPDLCs were exposed to different concentrations(100%，50% and 25%)of Super Bond, Clearfil SE Bond, G-Bond, Single Bond2, and Adper Easy One for different times (24 h, 48 h and 72 h). Cytotoxicity of 5 different agents to HPDLCs were observed by MTT method. The data was analyzed using SPSS 19.0 software package. RESULTS: Cytotoxicity of 5 dentin bonding agents to HPDLCs were Super BondP<0.05)but no significant difference was found between 48h and 72h(P>0.05). CONCLUSIONS: Cytotoxicity of 5 dentin bonding agents are different. Cytotoxicity of Super-bond C&B，G-Bond and Clearfil SE Bond are weak, while cytotoxicity of Single Bond2 is the strongest among the 5 agents.

Key words: Dental bonding agents, HPDLCs, Cytotoxicity

CLC Number:

R783.1

LI Jun-liang, WANG Wen-mei, GE Jiu-yu, JI Jun, WANG Tian-cong. A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2013, 22(6): 628-633.

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A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells

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