IL-12和Th亚群细胞在牙周病免疫病理及免疫调节中的作用

doi:10.19439/j.sjos.2020.05.014

摘要/Abstract

摘要： 目的：探讨白细胞介素12（interleukin-12,IL-12）在牙周病免疫病理及免疫调节中的作用。方法：选取2016年1月—2019年1月收治的98例慢性牙周炎患者,按照牙周炎严重程度分为轻度组（30例）、中度组（35例）与重度组（33例）,选取同期进行牙周检查的健康者30例作为对照组,观察各组间临床牙周指标[探诊深度（probing depth,PD）、附着丧失（attachment loss,AL）、菌斑指数（plague index,PLI）、出血指数（bleed index,BI）]、外周血中Th细胞表达（Th1、Th2、Th17）、龈沟液及血清中IL-12水平。采用SPSS 20.0软件包对数据进行统计学分析。结果：各组间PD、PLI、BI等指标相比差异有统计学意义（P<0.05）;轻、中、重度3组PD、PLI、BI显著高于对照组（P<0.05）;轻、中、重度3组AL指标相比差异显著（P<0.05）;中、重度组PD、AL、PLI、BI显著高于轻度组（P<0.05）,且重度组PD、AL、PLI、BI显著高于中度组（P<0.05）;轻、中、重度3组Th1、Th2、Th17显著高于对照组（P<0.05）;中、重度组Th1、Th2、Th17显著高于轻度组（P<0.05）,且重度组Th1、Th2、Th17显著高于中度组（P<0.05）;轻、中、重度龈沟液及血清中IL-12水平显著高于对照组（P<0.05）;中、重度组龈沟液及血清中IL-12水平显著高于轻度组（P<0.05）,且重度组龈沟液及血清中IL-12水平显著高于中度组（P<0.05）;龈沟液及血清中IL-12与PD、AL、PLI、BI、Th1、Th2及Th17等指标呈正相关（P<0.05）。H-E染色中、轻度组淋巴细胞较少,中度组淋巴细胞数目明显增加,重度组淋巴细胞表现为密集状态,同时在细胞间质中出血明显;IL-12蛋白阳性染色结果均在牙龈组织淋巴细胞质中表达,可见明显棕褐色,轻、中、重度3组IL-12蛋白阳性染色在牙龈组织中数量显著高于对照组,且着色随着轻、中、重度炎症变化而加重。结论：IL-12参与牙周病的免疫调节机制,可能是牙周炎发生的关键促炎细胞因子。

关键词: 慢性牙周炎, 白细胞介素12, 免疫病理, 免疫调节

Abstract: PURPOSE: To investigate the immunopathology and immunomodulatory roles of interleukin-12 (IL-12) in periodontal disease. METHODS: Ninety-eight patients with chronic periodontitis from January 2016 to January 2019 were enrolled and divided into mild group (30 cases), moderate group (35 cases) and severe group (33 cases) according to the severity of periodontitis; meanwhile, 30 healthy subjects who underwent periodontal examination in our hospital were selected as the control group. Clinical periodontal indicators including probing depth(PD), attachment loss(AL), plaque index(PLI), bleeding index(BI), Th cell expression (Th1, Th2, Th17) in peripheral blood, IL-12 levels in gingival crevicular fluid (GCF) and serum were measured. SPSS 20.0 software package was performed to analyze the correlation between IL-12 levels in GCF and serum and Th1, Th2, Th17, PD, AL, PLI, and BI. RESULTS: The differences of PD, PLI and BI among the groups were statistically significant(P<0.05). The levels of PD, PLI and BI in the mild, moderate and severe group were significantly higher than those in the control group (P<0.05). The difference of AL index among mild, moderate and severe group was statistically significant(P<0.05). The PD, AL, PLI, and BI in the moderate and severe group was significantly higher than those in the mild group(P<0.05), and the severe group was significantly higher than the mild group(P<0.05). Th1, Th2 and Th17 were significantly higher in the mild, moderate and severe group than in the control group(P<0.05); the moderate, severe group was significantly higher than the mild group in terms of Th1, Th2 and Th17 (P<0.05), and the severe group was significantly higher than the moderate group (P<0.05). The IL-12 levels in GCF and serum of the mild, moderate, and severe groups were significantly higher than those of the control group (P<0.05); IL-12 levels in the the moderate and severe groups were significantly higher than those in the mild group (P<0.05), and the IL-12 were significantly higher in the severe group than in the moderate group (P<0.05); IL-12 was positively correlated with PD, AL, PLI, BI, Th1, Th2 and Th17(P<0.05). H-E staining showed there were fewer lymphocytes in the mild group, more lymphocytes in the moderate group, and dense lymphocytes in the severe group with significant hemorrhage in intercellular mesenchyme. The IL-12 protein positive staining results were expressed in gingival tissue lymphocyte pulp with significant brown observed. The positive staining of IL-12 protein in the gingival tissues in the mild, moderate and severe group was significantly higher than in the control group, and the staining was aggravated with mild, moderate and severe inflammatory changes. CONCLUSIONS: IL-12 is involved in the immunoregulatory mechanism of periodontal disease and may be a key pro-inflammatory cytokine in the development of periodontitis.

Key words: Chronic Periodontitis, Interleukin-12, Immunopathology, Immune regulation

中图分类号:

R781.4

吴毓聪, 程亚楠, 陈绍山, 杨宾, 陆丽英. IL-12和Th亚群细胞在牙周病免疫病理及免疫调节中的作用[J]. 上海口腔医学, 2020, 29(5): 519-523.

WU Yu-cong, CHENG Ya-nan, CHEN Shao-shan, YANG Bin, LU Li-ying. Immunopathology and immunomodulatory roles of interleukin-12 in periodontal disease[J]. Shanghai Journal of Stomatology, 2020, 29(5): 519-523.

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