不同功率密度的姜黄素-光动力疗法对白色念珠菌生物膜的影响

doi:10.19439/j.sjos.2020.05.002

摘要/Abstract

摘要： 目的：探讨不同功率密度的姜黄素-光动力疗法对白色念珠菌生物膜的杀伤效果。方法：以白色念珠菌标准株和临床株为研究对象,进行体外不同功率密度（12、 22、 42、 62、 82、 102 mW/cm²）的姜黄素-光动力实验,通过XTT检测和激光共聚焦显微镜观察不同功率密度的姜黄素-光动力疗法对白色念珠菌生物膜的杀伤效果。采用SPSS 19.0软件包对数据进行统计学分析。结果：当姜黄素浓度为40 μmol/L,光照时间为4 min时,标准株和临床株的杀菌率均随着功率密度的增加而增大;功率密度增加至102 mW/cm²时,差异无统计学意义（P>0.05）。结论：姜黄素-光动力疗法杀伤白色念珠菌生物膜的效果与功率密度密切相关,建议将82 mW/cm²作为最佳功率密度。

关键词: 姜黄素, 光动力, 功率密度, 白色念珠菌, 生物膜

Abstract: PURPOSE: In this study, the inactivation effect of different fluence rates on Candida albicans biofilms during curcumin-photodynamic therapy was investigated in vitro. METHODS: The standard Candida albicans and clinical isolated Candida albicans were selected as model fungus and different fluence rates (12, 22, 42, 62, 82, 102 mW/cm²) during curcumin-photodynamic therapy were applied to inactivate Candida albicans biofilm. To evaluate the inactivation effect, XTT assay and Live/Dead kit were employed to quantify and visualize the activities of Candida albicans biofilms. The data were analyzed with SPSS 19.0 software package. RESULTS: When 40 μmol/L of curcumin was applied followed by 4 min illumination, both standard Candida albicans and clinical isolated Candida albicans biofilms were greatly inactivated along with the increase of fluence rates. When fluence rate increased to 102 mW/cm², there was no significant difference between the experimental group and the previous experimental group(P>0.05). CONCLUSIONS: Fluence rate plays an important role in inactivation of Candida albicans biofilms during curcumin-photodynamic therapy, with optimized value of fluence rate of 82 mW/cm² in this study.

Key words: Curcumin, Photodynamic, Fluence rate, Candida albicans, Biofilms

中图分类号:

R781.5

马婧, 李继扬, 杨巧珍, 白昱, 张程, 孙红英. 不同功率密度的姜黄素-光动力疗法对白色念珠菌生物膜的影响[J]. 上海口腔医学, 2020, 29(5): 456-461.

MA Jing, LI Ji-yang, YANG Qiao-zhen, BAI Yu, ZHANG Cheng, SUN Hong-ying. Effect of different fluence rates applied in curcumin-photodynamic therapy on Candida albicans biofilms[J]. Shanghai Journal of Stomatology, 2020, 29(5): 456-461.

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