人牙周膜干细胞成骨分化过程中P2X7受体mRNA的表达特点及作用研究

doi:10.19439/j.sjos.2020.04.006

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (4): 365-369.doi: 10.19439/j.sjos.2020.04.006

人牙周膜干细胞成骨分化过程中P2X7受体mRNA的表达特点及作用研究

李文静, 陈惠珍, 刘从娜, 张志勇

河北医科大学第二医院口腔内科,河北石家庄 050000

收稿日期:2019-09-16 修回日期:2019-10-23 出版日期:2020-08-25 发布日期:2020-09-11
通讯作者: 张志勇,E-mail:zhiyongzhang619@163.com
作者简介:李文静（1978-）,女,硕士,副主任医师,E-mail:sxqtsj@163.com

Expression level and effect of P2X7 receptor mRNA during osteogenic differentiation of human periodontal ligament stem cells

LI Wen-jing, CHEN Hui-zhen, LIU Cong-na, ZAHNG Zhi-yong

Department of Oral Medicine, the Second Hospital of Hebei Medical University. Shijiazhuang 050000, Hebei Province,China

Received:2019-09-16 Revised:2019-10-23 Online:2020-08-25 Published:2020-09-11

摘要/Abstract

摘要： 目的：探讨P2X7受体在人牙周膜干细胞成骨分化中的作用。方法：将原代培养获得的人牙周膜干细胞分为对照组、三磷酸腺苷组、成骨诱导液组和三磷酸腺苷+成骨诱导液组,比较4组的成骨分化相关基因RUNX2、OCN基因表达和P2X7受体mRNA表达的差异,采用SPSS 16.0软件包进行统计学分析。结果：成骨后1周和2周,三磷酸腺苷+成骨诱导液组的RUNX2和OCN mRNA表达显著高于成骨诱导液组（P<0.05）。三磷酸腺苷+成骨诱导液组成骨后1周的RUNX2和OCN mRNA表达显著高于成骨后2周（P<0.05）。成骨后1周和2周,三磷酸腺苷组和三磷酸腺苷+成骨诱导液组的P2X7受体mRNA表达均显著高于对照组和成骨诱导液组（P<0.05）;成骨后2周,三磷酸腺苷组的P2X7受体mRNA表达显著高于三磷酸腺苷+成骨诱导液组（P<0.05）;三磷酸腺苷组成骨后1周的P2X7受体mRNA表达显著高于成骨后2周（P<0.05）。结论：P2X7 受体明显提高牙周膜干细胞的成骨效果,三磷酸腺苷可激活P2X7 受体的表达。

关键词: P2X7 受体, 牙周膜干细胞, 三磷酸腺苷, 成骨分化

Abstract: PURSPOSE: To investigate the role of P2X7 receptor in osteogenic differentiation of human periodontal ligament stem cells. METHODS: Human periodontal ligament stem cells obtained from primary culture were divided into 4 groups: control group, adenosine triphosphate group, osteogenic induction group, adenosine triphosphate + osteogenic induction group. The differences of RUNX2, OCN gene expression and P2X7 receptor mRNA expression between the four groups were compared. Statistical analysis was performed with SPSS 16.0 software package. RESULTS: One week after osteogenic formation and two weeks after osteogenic formation, the expression of RUNX2 and OCN mRNA in the adenosine triphosphate + osteogenic induction group was significantly higher than that in the osteogenic induction group (P<0.05). The expression of RUNX2 and OCN mRNA in the 1 week after adenosine triphosphate + osteogenic induction fluid was significantly higher than that 2 weeks after osteogenic formation, and the difference was statistically significant (P<0.05). The expression of P2X7 receptor mRNA in the adenosine triphosphate group and the adenosine triphosphate + osteogenic induction group was significantly higher than that in the control group and the osteogenic induction group 1 week after osteogenesis and 2 weeks after osteogenesis (P<0.05). The expression of P2X7 receptor mRNA in the adenosine triphosphate group was significantly higher than that in the adenosine triphosphate + osteogenic induction group 2 weeks after osteogenesis(P<0.05). The expression of P2X7 receptor mRNA was significantly higher than that of osteogenic induction 1 week after adenosine triphosphate composition(P<0.05). CONCLUSIONS: P2X7 receptor can significantly improve the osteogenesis of periodontal ligament stem cells, and adenosine triphosphate can activate the expression of P2X7 receptor.

Key words: P2X7 receptor, Periodontal ligament stem cells, ATP, Osteogenic differentiation

中图分类号:

R781.4

李文静, 陈惠珍, 刘从娜, 张志勇. 人牙周膜干细胞成骨分化过程中P2X7受体mRNA的表达特点及作用研究[J]. 上海口腔医学, 2020, 29(4): 365-369.

LI Wen-jing, CHEN Hui-zhen, LIU Cong-na, ZAHNG Zhi-yong. Expression level and effect of P2X7 receptor mRNA during osteogenic differentiation of human periodontal ligament stem cells[J]. Shanghai Journal of Stomatology, 2020, 29(4): 365-369.

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人牙周膜干细胞成骨分化过程中P2X7受体mRNA的表达特点及作用研究

Expression level and effect of P2X7 receptor mRNA during osteogenic differentiation of human periodontal ligament stem cells

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