Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn

doi:10.19439/j.sjos.2021.05.005

Shanghai Journal of Stomatology ›› 2021, Vol. 30 ›› Issue (5): 472-476.doi: 10.19439/j.sjos.2021.05.005

• Original Articles • Previous Articles Next Articles

Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn

YAN Yi^1,2, YANG Zhi¹, WANG Li-qiang³, FU Yuan-fei¹

1. Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
2. Department of Stomatology, Xiangshan Hospital of Traditional Chinese Medicine, Huangpu District. Shanghai 200020;
3. State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University. Shanghai 200240, China

Received:2021-03-01 Revised:2021-04-16 Online:2021-10-25 Published:2021-11-08

Abstract

Abstract: PURPOSE: To investigate whether Ti-35Nb-2Ta-3Zr/Zn (TNTZ/Zn) composite material processed by friction stir processing has osteogenic activity. METHODS: Zn was added to the surface of TNTZ alloy by friction stir processing. The control group was TNTZ(TNTZ without FSP), the experimental groups was FSP(FSP without Zn), TNTZ/Zn-0.5 (0.5 mm pre-made holes) and TNTZ/Zn-1(1 mm pre-made holes). Surface characterization was performed by scanning electron microscope and X-ray diffraction. Alkaline phosphatase(ALP) activity of co-cultured rat bone marrow stromal stem cells (BMSCs) and COL-1α, OPN and OCN gene expression were detected by real-time quantitative PCR. SAS version 8.2 software was used for data analysis. RESULTS: Zn nanoparticles on the surface of the TNTZ/Zn-1 group were distributed homogeneously, with a diameter range of 70-80 nm. Compared with TNTZ group, the activity of ALP in TNTZ/Zn-0.5 and TNTZ/Zn-1 groups was up-regulated (P<0.05 and P<0.01). Compared with TNTZ group, ALP, COL-1α, OPN and OCN gene expression increased in TNTZ/Zn-0.5 and TNTZ/Zn-1 group, and the difference was statistically significant (P<0.05). CONCLUSIONS: Through FSP, Zn can be successfully integrated onto the surface of TNTZ alloy with nano-scale microstructures. TNTZ/Zn composite material can effectively induce bone formation and is a potential implant material.

Key words: Bone marrow stromal stem cells, Osteogenic activity, Friction stir processing

CLC Number:

R782.1

YAN Yi, YANG Zhi, WANG Li-qiang, FU Yuan-fei. Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn[J]. Shanghai Journal of Stomatology, 2021, 30(5): 472-476.

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Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn

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