Temporal and spatial expression of Swell1 gene in the development of mouse condyle cartilage

doi:10.19439/j.sjos.2021.05.003

Shanghai Journal of Stomatology ›› 2021, Vol. 30 ›› Issue (5): 462-466.doi: 10.19439/j.sjos.2021.05.003

• Original Articles • Previous Articles Next Articles

Temporal and spatial expression of Swell1 gene in the development of mouse condyle cartilage

CHEN Long¹, DAI Jie-wen¹, SHEN Guo-fang^1,2

1. Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
2. Shanghai University of Medicine & Health Sciences. Shanghai 201318, China

Received:2021-03-22 Revised:2021-05-10 Online:2021-10-25 Published:2021-11-08

Abstract

Abstract: PURPOSE: To explore the temporal and spatial expression pattern of Swell1 (LRRC8A) gene in mouse condylar cartilage. METHODS: By obtaining condyle samples of embryos at 15.5, 16.5, 18.5 days and newborn mice, H-E staining, immunofluorescence staining and qRT-PCR were used to explore the microstructure of mouse condyles and the temporal and spatial expression changes of genes related to cartilage development and Swell1 gene. Statistical analysis was performed using SPSS 25.0 software package. RESULTS: Swell1 gene expression began in the hypertrophic chondrocyte layer during the development of the condyle from embryonic day 16.5, and then gradually increased, and continued to be expressed during mouse embryonic development until the mouse was born. CONCLUSIONS: Swell1 is mainly expressed in hypertrophic chondrocytes during the development of mouse condyles, and it may be involved in the regulation of chondrocyte hypertrophy.

Key words: Mouse, Mandibular condyle, Swell1, Hypertrophic chondrocytes

CLC Number:

R782

CHEN Long, DAI Jie-wen, SHEN Guo-fang. Temporal and spatial expression of Swell1 gene in the development of mouse condyle cartilage[J]. Shanghai Journal of Stomatology, 2021, 30(5): 462-466.

References

[1] Hirouchi H, Kitamura K, Yamamoto M, et al.Developmental characteristics of secondary cartilage in the mandibular condyle and sphenoid bone in mice[J]. Arch Oral Biol, 2018, 89(1): 84-92.
[2] Yahara Y, Takemori H, Okada M, et al.Pterosin b prevents chondrocyte hypertrophy and osteoarthritis in mice by inhibiting sik3[J]. Nat Commun, 2016, 7: 10959.
[3] Sun MM, Beier F.Chondrocyte hypertrophy in skeletal development, growth, and disease[J]. Birth Defects Res C Embryo Today, 2014, 102(1): 74-82.
[4] Ji H, Li J, Shao J, et al.Histopathologic comparison of condylar hyperplasia and condylar osteochondroma by using different staining methods[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2017, 123(3): 320-329.
[5] Ripmeester EGJ, Timur UT, Caron MMJ, et al. Recent insights into the contribution of the changing hypertrophic chondrocyte phenotype in the development and progression of osteoarthritis [J]. Front Bioeng Biotechnol, 2018, 6: 18.e1-18.e25.
[6] Cahalan MD, Lewis RS.Role of potassium and chloride channels in volume regulation by T lymphocytes[J]. Soc Gen Physiol Ser, 1988, 43: 281-301.
[7] Osei-Owusu J, Yang J, Vitery MDC, et al.Molecular biology and physiology of volume-regulated anion channel (vrac)[J]. Curr Top Membr, 2018, 81: 177-203.
[8] Kumar L, Chou J, Yee CS, et al.Leucine-rich repeat containing 8a (lrrc8a) is essential for T lymphocyte development and function[J]. J Exp Med, 2014, 211(5): 929-942.
[9] Zhang Y, Xie L, Gunasekar SK, et al.Swell1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis[J]. Nat Cell Biol, 2017, 19(5): 504-517.
[10] Yamada T, Strange K.Intracellular and extracellular loops of lrrc8 are essential for volume-regulated anion channel function[J]. J Gen Physiol, 2018, 150(7): 1003-1015.
[11] Qiu Z, Dubin AE, Mathur J, et al.Swell1, a plasma membrane protein, is an essential component of volume-regulated anion channel[J]. Cell, 2014, 157(2): 447-458.
[12] Hunziker EB.Mechanism of longitudinal bone growth and its regulation by growth plate chondrocytes[J]. Microsc Res Tech, 1994, 28(6): 505-519.
[13] Song H, Park KH.Regulation and function of sox9 during cartilage development and regeneration[J]. Semin Cancer Biol, 2020, 67(Pt 1): 12-23.
[14] Lu P, Ding Q, Li X, et al.Swell1 promotes cell growth and metastasis of hepatocellular carcinoma in vitro and in vivo[J]. EBioMedicine, 2019, 48: 100-116.
[15] Chen L, König B, Stauber T.Lrrc8 channel activation and reduction in cytosolic chloride concentration during early differentiation of c2c12 myoblasts[J]. Biochem Biophys Res Commun, 2020, 532(3): 482-488.
[16] Rubino S, Bach MD, Schober AL, et al. Downregulation of leucine-rich repeat-containing 8a limits proliferation and increases sensitivity of glioblastoma to temozolomide and carmustine[J]. Front Oncol, 2018, 8: 142.e1-142.e12.

Temporal and spatial expression of Swell1 gene in the development of mouse condyle cartilage

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Recommended Articles

Metrics

Comments

[1]	SHI Qiu-tao, LU Hai-ping, XIAO Fang, JIANG Zhen, LIU Yi, GU Zhi-yuan. Condylar morphological changes before and after orthodontic treatment for angle Class I malocclusion adult patients [J]. Shanghai Journal of Stomatology, 2016, 25(3): 301-305.
[2]	XU Gao-li,WANG Shu-hua,SHI Qiu-tao,LIU Yi,YAN Fan,XU Ting,GU Zhi-yuan. The expression of VEGF-C in the condylar cartilage after anterior disc displacement and its clinical significance [J]. Shanghai Journal of Stomatology, 2016, 25(2): 136-140.
[3]	JING Jie,QU Ai-li,DING Xiao-mei,HEI Yu-na. Biomechanical analysis on healing process of sagittal fracture of the mandibular condyle after rigid fixation [J]. Shanghai Journal of Stomatology, 2015, 24(2): 164-169.
[4]	ZHANG Da-yong, DU Ning, LIU Yue-e, ZHANG Xiao-lu, WANG Si-jia. The experimental study of subcutaneous ectopic ossification with different proportional mixtures of human autologous bone and hydroxyapatite in nude mice [J]. Shanghai Journal of Stomatology, 2013, 22(6): 655-658.
[5]	QU Wei, LIU liu, DING Shi-mei, WANG She-jiao. Clinical analysis of radionuclide bone scan in patients with facial asymmetry [J]. Shanghai Journal of Stomatology, 2013, 22(3): 326-329.