Expression and correlation of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma

Abstract

Abstract: PURPOSE: To detect the expression of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma (OSCC) and explore their correlation with clinicopathologic parameters. METHODS: Protein levels of survivin and hsa-miR-542-3p were measured in 30 OSCC patients and SCC-15 cell line. Plasma hsa-miR-542-3p level was analyzed in 30 OSCC patients and 30 normal controls. Statistical analysis was performed with SPSS 17.0 software package. RESULTS: In OSCC samples, survivin was increased while hsa-miR-542-3p was decreased. There was a negative correlation between survivin and hsa-miR-542-3p. In SCC-15 cell line, inhibitor and mimic of hsa-miR-542-3p could regulate survivin expression. Plasma level of hsa-miR-542-3p was decreased in OSCC patients. The potential diagnostic performance of plasma hsa-miR-542-3p for OSCC patients was evaluated by ROC curve analysis and AUC was 0.82. CONCLUSIONS: Down-regulation of hsa-miR-542-3p correlates with increasing of survivin expression in OSCC. Plasma hsa-miR-542-3p is a potential diagnostic biomarker for OSCC.

Key words: Oral squamous cell carcinoma, MicroRNA, Survivin, Biomarker

CLC Number:

R739.8

WU Ning, LU Ying, LIANG Ji-zong. Expression and correlation of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2016, 25(6): 720-724.

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