Effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells

Abstract

Abstract: PURPOSE To analyze the effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells and to investigate the mechanism of migration. METHODS The inguinal adipose tissue of rat was excised at aseptic condition to obtain primary ADSCs by enzyme digestion. Multi-directional differentiation was used to identify the ADSCs. PRF membrane was acquired through one time centrifuge. The cell migration was examined by Transwell assay and wound healing assay. The mRNA expression of MMP2 and MT1-MMP was tested by real-time PCR. Statistical analysis was performed using SPSS 13.0 software package. RESULTS Cell migration test showed that the migration of rat ADSCs in PRF group were significantly higher than those in the negative group（P＜0.05） and inhibitor group（P＜0.05）. The ADSCs migration effects in three growth factors group at different concentrations showed significant difference（P＜0.05）. Real-time PCR showed that gene expressions of MMP2 and MT1-MMP were significantly higher in PRF group than control group (P＜0.05). PCR showed that gene expressions of MMP2 and MT1-MMP were significantly higher in three growth factors group than control group (P＜0.05). CONCLUTIONS: PRF and three growth factors consistently enhanced the migration of rat ADSCs in a dose-response manner. The migration increase of rat ADSCs may be associated with the up-regulation of MMP2 and MT1-MMP gene expression.

Key words: PRF, ADSCs, Migration, TGF-β1, PDGF-AB, VEGF

CLC Number:

R782

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GAO Jie, WANG Ming-guo, YANG Shuai, LI Xiu-mei, YANG Shi-mao, LI Xue.. Effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells[J]. Shanghai Journal of Stomatology, 2015, 24(6): 667-673.

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Effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells

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[1]	XU Fang-fang, JIANG Bei-zhan. Effect of DKK1 on the biological behaviors of dental pulp cells exposed to lipopolysaccharide [J]. Shanghai Journal of Stomatology, 2021, 30(4): 355-359.
[2]	WANG Zhen, SUN Yang, CHEN Jia-lu, GONG Yi-ming. Effect of curcumin on TGF-β1 /Smad3 pathway in rat gingival fibroblast treated with cyclosporine A [J]. Shanghai Journal of Stomatology, 2020, 29(4): 359-364.
[3]	LI Huai-qi, YE Jin-hai, DING Xu, WU He-ming. Effect of mi-138 targeting PLD2 gene on proliferation and migration of oral cancer cells [J]. Shanghai Journal of Stomatology, 2020, 29(1): 25-30.
[4]	FANG Shou-rong. Role of Bcl-2 and MIF proteins in senile patients with gingival cancer [J]. Shanghai Journal of Stomatology, 2020, 29(1): 105-108.
[5]	ZHAO Xian, ZENG Jin, ZUO Dong-chuan, XU Jie, YU Jing-hong, XU Xiao-mei. Effect of calcium on proliferation, migration and osteogenic differentiation of human dental follicle cells [J]. Shanghai Journal of Stomatology, 2019, 28(6): 572-577.
[6]	LANG Miao-jie, OU Yue-jian. The influence of zoledronic acid on proliferation, migration and apoptosis of fibroblasts [J]. Shanghai Journal of Stomatology, 2019, 28(5): 490-493.
[7]	LIN Zhe-jing, LUO Ren-hui, XIE Li-li, WU Xiao-yu. Effects of basic periodontal treatment on endothelin, vascular endothelial growth factor-A and tumor necrosis factor-α levels in gingival crevicular fluid and serum [J]. Shanghai Journal of Stomatology, 2019, 28(5): 504-508.
[8]	KANG Jian-yong, HE Juan, DUAN Xiao-feng. Biological effect of nicotine on Cal27 cell line in patients with tongue squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2019, 28(4): 368-372.
[9]	WANG Jing, FANGTENG Jiao-zi, LIU He. Effect of iRoot BP Plus on biological behavior of deciduous tooth pulp stem cells and human pulp stem cells [J]. Shanghai Journal of Stomatology, 2019, 28(3): 251-258.
[10]	WANG De-fang, LIU Yan, WANG Jue. rhPDGF-BB promotes migration of bone marrow stromal stem cells in diabetic rats [J]. Shanghai Journal of Stomatology, 2018, 27(6): 579-584.
[11]	SUN Yuan-qing, LIANG Bing, ZHANG Lei, XU Hui. Effects of sevoflurane on the migration of vascular endothelial cells [J]. Shanghai Journal of Stomatology, 2017, 26(3): 272-276.
[12]	CHENG Hui-juan, HUA Wei-lei, XU Kan. Occlusal adjustment treatment of pathological migration of the anterior teeth: report of one case [J]. Shanghai Journal of Stomatology, 2016, 25(5): 630-632.
[13]	XU Gao-li,WANG Shu-hua,SHI Qiu-tao,LIU Yi,YAN Fan,XU Ting,GU Zhi-yuan. The expression of VEGF-C in the condylar cartilage after anterior disc displacement and its clinical significance [J]. Shanghai Journal of Stomatology, 2016, 25(2): 136-140.
[14]	WANG Yu, TANG Guo-hua. Cyclic mechanical strain induces osteogenic differentiation in the coculture system of BMSCs and VECs [J]. Shanghai Journal of Stomatology, 2015, 24(5): 400-404.
[15]	LI Jing-dong, LIANG Rui-ying, ZHAO Yan-ping, XU Yan-li. Correlation analysis of VEGF and PTEN expression in gingival carcinoma [J]. Shanghai Journal of Stomatology, 2014, 23(5): 619-623.