The influences of non-surgical periodontal treatment on gingival crevicular fluid levels of MMP-8 and tissue inhibitor of TIMP-1

Abstract

Abstract: PURPOSE: To compare the levels of MMP-8 and TIMP-1 in the gingival crevicular fluid (GCF)of chronic periodontitis(CP) patients before and after non-surgical periodontal therapy. METHODS: Sulcus bleeding index (SBI), plaque index (PLI) and probing depth (PD) were detected before and after therapy. GCF was collected from CP patients before and after therapy. MMP-8 and TIMP-1 were determined by enzyme-linked immunoabsorbent assay. The data was analyzed using paired-sample t test and multi-correlation analysis with SSPS 19.0 software package. RESULTS: The levels of MMP-8 and TIMP-1 decreased after therapy, and there was no significant difference in the MMP-8/TIMP-1 ratio. Correction analysis showed that a significant correction was found between MMP-8 and GCF weight. CONCLUSIONS: MMP-8 and TIMP-1 decrease after non-surgical periodontal therapy.

Key words: Chronic periodontitis, GCF, MMP-8, TIMP-1

CLC Number:

R781.4

WANG Li-qin, FENG Kun, SUN Li. The influences of non-surgical periodontal treatment on gingival crevicular fluid levels of MMP-8 and tissue inhibitor of TIMP-1[J]. Shanghai Journal of Stomatology, 2014, 23(2): 229-232.

References

[1] Sapna G, Gokul S, Bagri-Manjrekar K. Matrix metalloproteinases and periodontal diseases [J]. Oral Dis, 2013 Jul 2. [Epub ahead of print]
[2] Nagase H, Visse R, Murphy G. Structure and function of matrix metalloproteinases and TIMPs [J]. Cardiovasc Res, 2006, 69(3): 562-573.
[3] Hannas AR, Pereira JC, Granjeiro JM, et al. The role of matrix metalloproteinases in the oral environment [J]. Acta Odontol Scand, 2007, 65(1): 1-13.
[4] Golub LM, Lee HM, Stoner JA, et al. Subantimicrobial-dose doxycycline modulates gingival crevicular fluid biomarkers of periodontitis in postmenopausal osteopenic women[J]. J Periodontol, 2008, 79(8): 1409-1418.
[5] Marcaccini AM, Meschiari CA, Zuardi LR, et al. Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy [J]. J Clin Periodontol, 2010, 37(2): 180-190.
[6] Meschiari CA, Marcaccini AM, Santos Moura BC, et al. Salivary MMPs, TIMPs, and MPO levels in periodontal disease patients and controls [J]. Clin Chim Acta, 2013, 421: 140-146.
[7] Rathnayake N, Akerman S, Klinge B, et al. Salivary biomarkers of oral health: a cross-sectional study [J]. J Clin Periodontol, 2013, 40(2): 140-147.
[8] Okuda K, Miyazaki A, Momose M, et al. Levels of tissue inhibitor of metalloproteinases-1 and matrix metalloproteinases-1 and -8 in gingival crevicular fluid following treatment with enamel matrix derivative [J]. J Periodontal Res, 2001, 36(5): 309-316.
[9] Marcaccini AM, Meschiari CA, Zuardi LR, et al. Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy [J]. J Clin Periodontol, 2010, 37(2): 180-190.
[10] Mouzakiti E, Pepelassi E, Fanourakis G, et al. Expression of MMPs and TIMP-1 in smoker and nonsmoker chronic periodontitis patients before and after periodontal treatment [J]. J Periodontal Res, 2012, 47(4): 532-542.
[11] Marcaccini AM, Novaes AB Jr, Meschiari CA, et al. Circulating matrix metalloproteinase-8 (MMP-8) and MMP-9 are increased in chronic periodontal disease and decrease after non-surgical periodontal therapy [J]. Clin Chim Acta, 2009, 409(1-2): 117-122.
[12] Letra A, Silva RM, Rylands RJ, et al. MMP3 and TIMP1 variants contribute to chronic periodontitis and may be implicated in disease progression [J]. J Clin Periodontol, 2012, 39(8): 707-716.
[13] 曹采方.牙周病学[M]. 第2版.北京:人民卫生出版社, 2003: 75.

[1]	XU Jia-li, SUN Kan-da, ZHU Ya-qi. Effect of grape seed extract on oxidative stress and pathological changes of aorta in rats with chronic periodontitis and arteriosclerosis [J]. Shanghai Journal of Stomatology, 2022, 31(6): 597-601.
[2]	DING Yuan-sen, SHAO Zheng-wei, LIN Zong-jian, ZOU De-rong. Periodontal health survey and analysis of influencing factors in 916 patients with type 2 diabetes [J]. Shanghai Journal of Stomatology, 2022, 31(2): 173-177.
[3]	WU Yu-cong, CHENG Ya-nan, CHEN Shao-shan, YANG Bin, LU Li-ying. Immunopathology and immunomodulatory roles of interleukin-12 in periodontal disease [J]. Shanghai Journal of Stomatology, 2020, 29(5): 519-523.
[4]	QU Dong-lin, GU Yan-lin, LIU Yi, LIAO Qian. Effect of continuous oral health education on elderly patients with chronic periodontitis treated with dual-wavelength laser [J]. Shanghai Journal of Stomatology, 2019, 28(3): 321-324.
[5]	LIU Qiu-yue, ZHANG Yi, LI Jing, XIANG Xue-rong. Locally delivered macrolides as an adjunct to non-surgical periodontal treatment of chronic periodontitis: a meta analysis [J]. Shanghai Journal of Stomatology, 2019, 28(2): 207-217.
[6]	LI Feng, XU Hua-shun. Effects of low level laser combined with basic periodontal therapy on cytokines and LPS, leptin in gingival crevicular fluid of diabetes mellitus complicated with chronic periodontitis patients [J]. Shanghai Journal of Stomatology, 2018, 27(6): 637-640.
[7]	LUO Qiang, GU Xing-hua. Expression of cytokines Il-6, IL-34 and M-CSFR in chronic periodontitis and its clinical significance [J]. Shanghai Journal of Stomatology, 2018, 27(6): 652-656.
[8]	ZHONG Zhao-qi, FU Hua. Effects of SPR periodontal basic therapy on cervical crevicular fluid C-reactive protein in patients with chronic periodontitis [J]. Shanghai Journal of Stomatology, 2018, 27(5): 530-534.
[9]	GAO Ying, HAO Chen-di.. Expression of miR-146a in saliva of chronic periodontitis patients and its influence on gingival crevicular inflammation and MMP-8/TIMP-1 levels [J]. Shanghai Journal of Stomatology, 2018, 27(3): 309-312.
[10]	SUN Xiao-na, SONG Ai-mei, YANG Pi-shan. Short-term effect of ibuprofen on clinical indexes and cytokines in the gingival crevicular fluid of patients with severe chronic periodontitis [J]. Shanghai Journal of Stomatology, 2017, 26(5): 557-560.
[11]	YAN Ying, XIANG Xue-rong, WANG Chun, YE Guo, FAN Xiao-ping, YANG Mei. Clinical significance of calcitonin gene-related peptide level before and after treatment in patients with chronic periodontitis [J]. Shanghai Journal of Stomatology, 2016, 25(4): 448-452.
[12]	LIU Zhen-yu, ZHANG Jing-dong, ZHANG Lin, YANG Jian, LIU Xiang-hui. Analysis of minocycline hydrochloride combined with Vitapex in treating senile chronic periodontal-endodontic combined lesions [J]. Shanghai Journal of Stomatology, 2016, 25(4): 465-468.
[13]	HE Yan-ping, JIAO Ting.. Expressions of EMMPRIN and its ligand CyPA in gingival crevicular fluid of chronic periodontitis patients [J]. Shanghai Journal of Stomatology, 2016, 25(1): 27-31.
[14]	FAN Yun, CHEN Dong, YE Xin, YU Fang-fang, LI Wei.. The impact of smoking on human beta defensin 2,3 in gingival crevicular fluid and gingival tissue of patients with chronic periodontitis [J]. Shanghai Journal of Stomatology, 2015, 24(6): 735-738.
[15]	LIU Di-xin, JIN Ying, LIN Xiao-ping. Expression and correlation of IL-35 in gingival crevicular fluid and serum with chronic periodontitis [J]. Shanghai Journal of Stomatology, 2015, 24(4): 455-459.