An in vitro evaluation of the effect of carboxymethyl chitosan and its composites against Enterococcus faecalis in the root canal

Abstract

Abstract: PURPOSE: To evaluate the bacteriostasis effect of carboxymethyl chitosan and its composites on intracanal E. faecalis. METHODS: The pattern of E. faecalis infecting root canal was established, and then divided into 4 groups(A, B, C and D).They were filled with 2% chlorhexidine solution, 140 mg/mL mixture of carboxymethyl chitosan and chlorhexidine solution, 5 mg/mL carboxymethyl chitosan solution and calcium hydroxide paste respectively and incubated for 7 days. Samples obtained before and after the intracanal medication were plated onto BHI media to determine the colony-forming units (CFU/mL) after 48 hours. The data were analysed using SPSS 13.0 software package. RESULTS: Before intracanal medication, the variance of bacterial counts were not significantly different (P>0.05). After medication, the four groups showed significant difference in bacterial counts immediately(P<0.05).The antimicrobial effects of A and B group were better than group C and D. CONCLUSIONS: The antibiotic activity of 2% chlorhexidine solution and 140 mg/L mixture of carboxymethyl chitosan and chlorhexidine solution to E.faecalis were better.

Key words: Enterococcus faecalis, Carboxymethyl chitosan, Chlorhexidine;Calcium hydroxide past

CLC Number:

R781.3

SONG Xue-lian, SUN Ying-ying, LIU Yang, CHEN Qing, BI Xin-xin, YU Jing-tao. An in vitro evaluation of the effect of carboxymethyl chitosan and its composites against Enterococcus faecalis in the root canal[J]. Shanghai Journal of Stomatology, 2013, 22(3): 265-269.

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