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    25 December 2016, Volume 25 Issue 6 Previous Issue    Next Issue
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    Construction of leptin gene modified tissue engineered composites in vitro
    ZHENG Bao-yu, HE Meng-jiao, JIANG Jun, LUO Kai, CHEN Yu-ling, YAN Fu-hua
    2016, 25 (6):  641-646. 
    Abstract ( 201 )   HTML ( 2 )   PDF (1561KB) ( 191 )  
    PURPOSE: To evaluate the feasibility of constructing tissue engineered composites in vitro by combining human leptin (hLEP) gene modified rat bone marrow stromal cells (BMSCs) and guided tissue regeneration collagen membrane (Bio-Gide). METHODS: BMSCs of SD rats were isolated and cultured by whole bone marrow adherent method. BMSCS were transfected with adenovirus carrying hLEP gene (Ad-hLEP-EGFP) and observed under inverted fluorescence microscope. Enzyme linked immunosorbent assay (ELISA) was used to detect the expression of hLEP. The proliferation activity of transfected cells was assessed by MTT assay. Ad-hLEP-EGFP transfected BMSCs were cultured for 24 h in combination with Bio-Gide collagen membrane, hLEP modified tissue engineered composite was observed under laser scanning confocal microscope (LSCM) and scanning electron microscope (SEM). RESULTS: Through Ad-hLEP-EGFP transfection, hLEP was overexpressed in BMSCs, which didn't affect the proliferation of cells. SEM showed hLEP modified BMSCs grew well on Bio-Gide collagen membrane and secreted extracellular matrix. LSCM suggested BMSCs could migrate to different scales of Bio-Gide collagen membrane. CONCLUSIONS: hLEP modified BMSCs can be combined with Bio-Gide collagen membrane and grow well, suggesting that hLEP modified tissue engineered composite can be successfully constructed. The composite might be suitable for periodontal tissue engineering.
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    Effects of strontium ranelate on the expression of BMP-2 in the mid-palatal suture of rats during rapid maxillary expansion
    XU Chao, WANG Xu-xia, WANG Ying-zi, YANG Pan-pan, ZHAO Shu-ya, ZHANG Jun
    2016, 25 (6):  647-651. 
    Abstract ( 183 )   HTML ( 1 )   PDF (1508KB) ( 202 )  
    PURPOSE: To investigate the effects of strontium ranelate on the expression of BMP-2 during rapid maxillary expansion. METHODS: Thirty-six male 6-week- old male Wistar rats were selected. They were randomly divided into 3 groups. Group A was designed as a control group. An expanded application producing 100 g force was fixed between the first and the second molar on both sides of the rats in group B and group C. 600 mg/kg strontium ranelate was given to the rats in group C daily via an orogastric route, while the equal normal saline was given to the rats in group B. Then the rats were sacrificed on day 4, 7 and 10. The expression of BMP-2 which is a sign of bone formation was detected with immunohistochemical staining and analyzed with Image-pro plus 5.0. SPSS19.0 software package was used for statistical analysis. RESULTS: The expression of BMP-2 in the midpalatal suture was significantly greater in group B than that in group A on day 4 (P<0.05); however, there was no significant difference between group A and group B on day 7 and 10 (P>0.05). The expression of BMP-2 in the midpalatal suture in group C was significantly greater than that in the other two groups at each time point (P<0.05). CONCLUSIONS: Strontium ranelate promotes the expression time and quantity of BMP-2 in the mid-palatal suture of rats during rapid maxillary expansion and may accelerate bone formation during rapid maxillary expansion.
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    Expression of STAT3 in alveolar bone during orthodontic tooth movement in rats
    ZHOU Si-ru, DAI Qing-gang, ZHANG Peng, HA Na-yong, YANG Shu-liang, JIANG Ling-yong
    2016, 25 (6):  652-656. 
    Abstract ( 193 )   HTML ( 3 )   PDF (1170KB) ( 197 )  
    PURPOSE: To study the changes of expression of signal transducer and activator of transcription 3 (STAT3) during orthodontic tooth movement (OTM). METHODS: Twenty six-week-old SD rats were selected, the upper left first molars were moved by coil spring and lasted for 7 days. The maxillary tissues were obtained at 0, 1, 3, 7 d and subjected to histological study. Statistical analysis was performed using SPSS 16.0 software package. RESULTS: The main changes in alveolar bone during orthodontic tooth movement included significant increase in osteoblast number and bone formation in the tension area, and bone resorption in the pressure area. The positive cells of osteocalcin in the tension area increased during OTM. The expression of STAT3 increased in the tension area at 3 d and 7 d in comparison with that at 0 d. CONCLUSIONS: Orthodontic force can stimulate alveolar bone remolding. The expression of STAT3 in the tension area may have effects on alveolar bone remolding during orthodontic tooth movement.
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    Histological evaluations on periapical tissues after irradiation by erbium-doped yttrium aluminum garnet laser in Labradors dogs
    WANG Hao-ming, ZHOU Meng-qi, HONG Jin
    2016, 25 (6):  657-662. 
    Abstract ( 202 )   HTML ( 3 )   PDF (2281KB) ( 192 )  
    PURPOSE: To investigate the thermal effects on periapical tissues of Labrador dogs after intra-canal irradiation by erbium-doped yttrium aluminum garnet (Er:YAG) laser at different powers based on the antibacterial experiment of Enterococcus faecalis and Escherichia coli in root canals with an isthmus, to assess the histological changes, and to prove the safety for clinical applications. METHODS: Two hundred root canals of 10 healthy adult Labradors dogs were selected and divided into 5 groups. Excepted one as control group, root canals in other 4 groups were irradiated by Er:YAG laser with 1.5, 2.0, 2.5, 3.0 W for 30 s. Ten Labradors dogs were sacrificed at 0 (immediately after irradiation), 2 days, 2 weeks, 1 and 2 months. After preparation of pathological specimen, histological changes after laser irradiation in periapical tissues were evaluated and scores of inflammation were graded. Statistical analysis was performed using SPSS 13.0 software package. RESULTS: No significant difference was observed at the apical area between 1.5, 2.0, 2.5 W and control groups at most periods (P>0.05), whereas significant difference was observed between 3.0 W and other groups for all periods (P<0.05). CONCLUSIONS: These results suggest that if proper output powers (2.0-2.5 W) of laser irradiation are chosen, disinfection in root canals can be finished successfully and thermal effects on the periapical tissues can be minimized.
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    Effects of repeated firing on microleakage of selective laser melting ceramic crowns
    ZHONG Qun, PENG Yan, WU Xue-ying, WENG Jia-wei
    2016, 25 (6):  663-667. 
    Abstract ( 278 )   HTML ( 1 )   PDF (909KB) ( 776 )  
    PURPOSE: To investigate the effects of repeated firing on microleakage of selective laser melting ceramic crowns. METHODS: Fifty molars were randomly divided into 2 groups (25 teeth in each group). Teeth in group A received a chamfer finish line preparation, whereas teeth in group B received a shoulder finish line. After SLM metal crowns were fabricated, all the crowns received initial oxidation step, opaque firing, dentin firing and glaze firing, then crowns in each group were randomly divided into 5 sub-groups according to different time of clinical firings. Glass ionomer was applied for bonding. After 5000 thermocycles ranging from 5℃ to 55℃, all the specimens was evaluated by dye penetration and then microleakage was examined under light microscopy. The data were analyzed with SPSS 20.0 software package. RESULTS: Microleakage between all specimens of group A were not statistically significant (P>0.05) whereas that of group B were statistically significant (P<0.05); After the fifth time of clinical firing, microleakage of specimens in group B(B5) were significantly higher than that of group A(A5). CONCLUSIONS: Repeated firings had no significant influence on marginal microleakage of SLM ceramic crowns whereas the crowns of chamfer finish lines result in better clinical performance after repeated firings.
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    Comparative study of microleakage by using different finished lines in selective laser melting metal crowns
    PENG Yan, ZHONG Qun, WU Xue-ying, Weng Jia-wei
    2016, 25 (6):  668-672. 
    Abstract ( 138 )   HTML ( 1 )   PDF (990KB) ( 210 )  
    PURPOSE: To evaluate microleakage of SLM Co -Cr alloy metal crown with two types finished line (chamfer and shoulder), compared with conventional fabrication of Co -Cr alloy metal crowns. METHODS: Thirty healthy non-carious human molars were selected and randomly assigned to 3 groups, 10 in each. Teeth in group A and C received a chamfer finish line preparation, whereas teeth in group C received a shoulder finish line. Conventional Co -Cr alloy metal crowns were fabricated for group A when SLM metal crowns were made for group B and group C. Glass ionomer was applied for bonding. After 5000 thermocycles ranging from 5℃ to 55℃,all the specimens were evaluated by dye penetration and then microleakage was examined under light microscope. The data were analyzed statistically with SPSS 20.0 software package. RESULTS: Microleakage in group A was significantly higher than the other two groups, group B and group C showed no significant difference in microleakage while microleakage in group B was higher than that in group C. CONCLUSIONS: Microleakage of SLM metal crowns was significantly less than that of conventional Co-Cr alloy metal crowns; chamfer finish line designs was recommended for SLM metal crowns in consideration of reducing microleakage and protecting tooth.
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    Drug release from electrospun simvastatin/polycaprolactone fibrous membranes
    ZHAO Bing-jiao, XU Yao, LIU Yue-hua
    2016, 25 (6):  673-677. 
    Abstract ( 156 )   HTML ( 1 )   PDF (983KB) ( 235 )  
    PURPOSE: To prepare electrospun simvastatin/polycaprolactone(SMV/PCL) membrane scaffolds and to evaluate the release properties of this formulation. METHODS: Electrospun SMV/PCL membrane scaffolds were prepared as the experimental group, and electrospun PCL membrane as the control group. The morphology and characteristics of membrane surface were determined by scanning electron microscopic(SEM) and X-ray diffraction(XRD). The release profile of SMV was determined using an ultraviolet-visible spectrometer. The data were analyzed statistically with SPSS 17.0 software package. RESULTS: SEM and XRD indicated that SMV/PCL nanofibers were successfully electrospun and SMV was encapsulated into the fibers. In vitro drug release studies showed that simultaneous SMV release, being nearly linear with time, was achieved and sustained SMV release was prolonged to 28 days. CONCLUSIONS: Electrospun SMV/PCL nanofiber membranes demonstrate sustained drug release properties, suggesting their potential applicability as prospective scaffolds in periodontal regeneration.
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    Preparation and identification of polyclonal antibody of Streptococcus salivarius 57.I urease
    WANG Yan, TAO Dan-ying, FENG Xi-ping
    2016, 25 (6):  678-681. 
    Abstract ( 249 )   HTML ( 2 )   PDF (547KB) ( 169 )  
    PURPOSE: To prepare and identify polyclonal antibody of Streptococcus salivarius 57.I urease. METHODS: The biggest structural subunit of Streptococcus salivarius 57.I urease, UreC, was obtained by gene clone, IPTG-induced expression, and purification through affinity chromatography. Anti-sera and polyclonal antibody were raised by immunizing rabbits with purified UreC. Western blot was utilized to detect the specific combination of polyclonal antibody with UreC. RESULTS: Purified UreC protein was prepared and used as antigen to immunize rabbits. Polyclonal antibody was obtained, and Western blot displayed a specific band of the polyclonal antibody with UreC about 62 kD as anticipated. CONCLUSIONS: Polyclonal antibody against Streptococcus salivarius 57.I urease is obtained, which provides an important tool to explore the function of urease and its relationship with dental caries.
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    MiR-142-3p inhibits lipopolysaccharide-induced inflammatory response in human periodontal ligament cells through targeting IRAK1
    HU Hai-yan, WANG Jun-qiang
    2016, 25 (6):  682-687. 
    Abstract ( 113 )   HTML ( 1 )   PDF (1174KB) ( 187 )  
    PURPOSE: To investigate the effects of miR-142-3p overexpression on secretion of inflammatory factors in human periodontal ligament cells (hPDLCs) induced by lipopolysaccharide (LPS) and its underlying mechanism. METHODS: hPDLCs were cultured by tissue cultivation in vitro and treated with various concentrations of LPS for 6, 12, 24 and 48 h. hPDLCs were treated with miR-142-3p mimics for 24 h in the presence of 2.0 μg/mL LPS and the expression levels of inflammatory factors (TNF-α, IL-6 and IL-1β) were detected by ELISA assay. The target relationship between miR-142-3p and interleukin-1 receptor- associated kinase 1 (IRAK1) was detected by dual-luciferase reporter assay. The miR-142-3p and IRAK1 mRNA expression was measured by qRT-PCR. The protein expression levels of IRAK1, TLR4 and NF-κB were detected by Western blotting. Statistical analysis was performed using SPSS 16.0 software package. RESULTS: The miR-142-3p expression level was decreased significantly at 24 h in 2.0 μg/mL LPS-treated hPDLCs (P<0.01). Transfection of miR-142-3p mimics remarkably increased miR-142-3p expression (P<0.01). The secretion of inflammatory factors (TNF-α, IL-6 and IL-1β) in LPS-treated hPDLCs was significantly decreased by miR-142-3p overexpression (P<0.05). Dual-luciferase reporter assay showed a directly targeting relationship between miR-142-3p and IRAK1. Overexpression of miR-142-3p significantly reduced the IRAK1 protein expression in LPS-treated hPDLCs (P<0.05), whereas no obvious effect on IRAK1 mRNA expression was noted (P>0.05). Moreover, miR-142-3p overexpression markedly diminished the protein expression of TLR4 and phosphorylated NF-κB in LPS-treated hPDLCs (P<0.05). CONCLUSIONS: Overexpression of miR-142-3p can alleviate the inflammatory response induced by LPS in hPDLCs and the underlying mechanism may be associated with targeting and inhibiting IRAK1 expression leading to suppression of TLR4/NF-κB inflammatory signaling pathway through which miR-142-3p overexpression protects peridentium against inflammation.
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    Knockdown of Grb7 inhibits growth of oral squamous cell carcinoma, cell proliferation and promoted apoptosis through ERK/FOXM1 pathway
    LIU Bing-yao, CAO Gang, DONG Zhen, CHEN Wei, XU Jin-ke, ZHANG Sen-lin, WENG Zhi-qiang
    2016, 25 (6):  688-693. 
    Abstract ( 217 )   HTML ( 1 )   PDF (1135KB) ( 181 )  
    PURPOSE: To investigate the effect of growth factor receptor-bound 7 (Grb7) on oral squamous cell carcinoma growth and tumor xenografts. METHODS: Cal27 and hNOK cells were cultivated, real-time PCR and Western blotting were used to detect the expression of Grb7 in hNOK and Cal27. Cal27 was transfected with Grb7 siRNA for 48 h, cell proliferation was assayed using MTT. Flow cytometry was used to determine the cell cycle and apoptosis. Western blot was used to evaluate the expression of caspase3, Bax, bcl-2, Cyclin D1, Rb, E2F1, ERK and FOXM1. Grb7 siRNA and negative control were designed and injected subcutaneously into the mice, tumor volume and weight were measured. Statistical analysis was performed using SPSS 11.0 software package. RESULTS: Grb7 was highly expressed in Cal27 compared with hNOK. Depletion of Grb7 significantly inhibited cell proliferation, blocked G1/S phase transition, promoted cell apoptosis. Knockdown of Grb7 suppressed the expression of Cyclin D1 and Rb, upregulated E2F1 expression. Moreover, c-caspase 3 and Bax was also reduced after inhibition of Grb7. ERK/FOXM1 signaling pathway was also inhibited by Grb7. In addition, the volume and weight of tumor xenografts were reduced by siGrb7. CONCLUSIONS: Depletion of Grb7 inhibits cell proliferation, promotes apoptosis and reduces tumor xenografts through ERK/FOXM1 pathway.
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    Comparison of condylar position between Angle Class I and Class Ⅱ malocclusion in teenagers
    GU Yong-jia, LU Sheng-nan, GAO Mei-qin, GU Xin-yu, SHI Hui, MA Jun-qing
    2016, 25 (6):  694-696. 
    Abstract ( 258 )   HTML ( 3 )   PDF (631KB) ( 168 )  
    PURPOSE: To analyze and compare the difference of condylar position between Angle Class I and Class Ⅱ malocclusion patients using cone-beam CT (CBCT). METHODS: Thirty Class I patients, 30 Class Ⅱ division 1 patients and 30 Class Ⅱ division 2 patients were selected in this study. Each patient underwent CBCT. The images in the oblique position perpendicular to the condyloid process were reconstructed by Examvision software. The joint space was measured by Exam Vision software. The data were processed with SPSS17.0 software package. RESULTS: The upper joint space was larger in Class Ⅱ, the posterior joint space was smaller in Class Ⅱ patients; and in Class Ⅱ division 1 patients, both of the upper and anterior joint spaces were larger than in Class Ⅱ division 2 patients,the differences were significant (P<0.05). The length of condyle was longer in Class I patients than in Class Ⅱ patients. CONCLUSIONS: The condylar position in Class Ⅱ division 2 patients was lower and further backward. The length of condyle is shortest in Class Ⅱ division 2 patients.
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    Periodontal status of labially inversely impacted maxillary central incisor in mixed dentition after orthodontic treatment
    JIANG Hui-xi, CAO Zhen-sheng, SUN Hao, HU Han, FANG Yu, HU Rong-dang
    2016, 25 (6):  697-701. 
    Abstract ( 228 )   HTML ( 1 )   PDF (979KB) ( 191 )  
    PURPOSE: To study the periodontal status of labially inversely impacted maxillary central incisor in mixed dentition after orthodontic treatment. METHODS: Sixteen patients with labially inversely impacted maxillary central incisor in mixed dentition and treated by guided rod appliance and conventional fixed appliance were enrolled in this study. Periodontal examinations were performed and cone-beam CT (CBCT) was taken approximately 2 years post treatment. Central incisors were divided into impacted and contralateral incisor groups. Clinical examinations included plaque index (PLI), gingival index (GI), probing depth (PD), width of attached gingiva (AW) and clinical crown length (CL); Mimics 16.0 software was used to measure the length of alveolar bone around the root (ha and fc), the length from the alveolar bone crest to cemento-enamel junction(CEJ) (ab and cd) on both the labial and palatal side of the central incisors as well as the labial and palatal bone support proportion (LBP and PBP) of the root and tipping of central incisor (U1-SN). The data were analyzed using paired sample t tests with SPSS 17.0 software package. RESULTS: There was no significant difference in PLI, GI, PD, U1-SN; the value of AW, ha,fc, LBP, PBP was significantly smaller in impacted groups(P<0.01); the value of CL, ab and cd was significantly larger in impacted groups (P<0.01). CONCLUSIONS: The periodontal status of the impacted central incisor was on good condition without teeth mobility and inflammation, although a little worse than the contralateral homonymous teeth. There may be a need for periodontal bone grafting and gingival surgery in the future for the asymmetric morphology of gingiva.
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    Relationship between upper airway and cranial-facial growth patterns in adolescents with normal occlusion
    ZHANG Qi-feng, JIN Li, YAO Hua, LI Zhi-yong, HUANG Xu, WANG Hui-ming
    2016, 25 (6):  702-706. 
    Abstract ( 191 )   HTML ( 3 )   PDF (814KB) ( 244 )  
    PURPOSE: To investigate the relationship between upper airway and cranial-facial growth pattern among teenagers with normal occlusion. METHODS: The study subjects consisted of 52 boys and 64 girls with normal occlusion. Their cephalometric radiographs were obtained. The sagittal diameter of upper airway was analyzed according to gender and different growth pattern. The relationship of upper airway diameter and cranial-facial formation was evaluated by stepwise regression analysis using SPSS 21.0 software package. RESULTS: There was no significant difference between male and female about upper airway diameter in teenagers, also no difference was found among different growth patterns. Regression analysis showed that upper airway was associated with some cranial-facial formation parameters. CONCLUSIONS: Although there is no significant difference in the upper airway diameters among different growth patterns, there is still certain association between cranial-facial formation and upper airway in adolescents.
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    Correlations of plasma concentrations of β-amyloid peptide and S-100β with postoperative cognitive dysfunction in patients undergoing oral and maxillofacial cancer surgery
    LIANG Bing, SUN Yuan-qing, JIANG Jue, XU Hui
    2016, 25 (6):  707-710. 
    Abstract ( 165 )   HTML ( 2 )   PDF (531KB) ( 191 )  
    PURPOSE: To evaluate the changes of perioperative plasma concentrations of Aβ1-40 and S-100β to determine the relationship with postoperative cognitive dysfunction in elderly patients undergoing oral and maxillofacial cancer surgeries. METHODS: One hundred and fifteen patients aged at least 60 years undergoing oral and maxillofacial tumor resection were investigated between May 2014 to December 2014.Neuropsychological tests for detecting postoperative cognitive dysfunction(POCD) were performed one day before surgery and 7 days postoperatively. According to the results of neuropsychological tests on day 7, patients were divided into POCD group and non-POCD group.Plasma values of Aβ1-40 and S-100β were determined with enzyme linked immunosorbent assay (ELISA) before anesthesia induction, 24 h and 7 days after surgery. The data were analyzed using SPSS 19.0 software package. RESULTS: According to the definition, POCD was present in 37 of 115 (32.3%) patients 1 week after surgery. Compared with pre-anesthesia, S-100β levels in POCD group were significantly increased (P<0.05); the level of Aβ1-40 was significantly higher 24 h after surgery (P<0.05). Compared with non-POCD group, S-100β levels were significantly increased 24 h postoperatively (P<0.05); Aβ1-40 levels were significantly higher 24 h and 7 days postoperatively (P<0.05). CONCLUSIONS: POCD was present in 32.2% of patients on day 7 after oral and maxillofacial surgeries with general anesthesia. The increasing levels of Aβ1-40, S-100β may be associated with the occurence of POCD. Patients with long-lasting operation and high concentrations of Aβ1-40 and S-100β after surgeries were at a higher risk of POCD. The clinical values of Aβ1-40 and S-100 as predictive measurements of POCD after oral and maxillofacial cancer surgery appear to be reasonable.
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    Influential factors of dental anxiety among dental implantation patients
    CHEN Yang, LIU Min
    2016, 25 (6):  711-714. 
    Abstract ( 249 )   HTML ( 2 )   PDF (459KB) ( 172 )  
    PURPOSE: To explore the influential factors and of dental anxiety among dental implantation patients. METHODS: We conducted a dental anxiety survey in 226 patients, and analyzed the relationship of different factors with dental anxiety, including education, age, gender, number of implantation, systematic diseases, and analyzed source of dental anxiety. SPSS 17.0 software package was used for data analysis. RESULTS: Education, number of implantation, systematic diseases were the influential factors for dental anxiety. Women were more serious to medical staff. Instrument, low education level showed higher score of anxiety for medical staff and therapeutic efficacy. The more number of implantation the more serious to anxiety for efficacy, and patients with systematic disease presented extreme worries to clinical efficacy. CONCLUSIONS: Patients with different education background, number of implantation, systematic diseases have different levels dental of anxiety, doctors should pay more attentions to higher risk of dental anxiety patients according to different influential factors, and pertinence preventive measures should be adopted.
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    Interforaminal regions of 287 Han adults evaluated by cone-beam computed tomography
    ZENG Ting-wen, GAO Qing-ping, GAO Xiao-meng, YU Hui-min, WU Yi-wei
    2016, 25 (6):  715-719. 
    Abstract ( 224 )   HTML ( 1 )   PDF (873KB) ( 139 )  
    PURPOSE: The mandibular anatomical structures including mental foramen, accessory mental foramina, anterior loop were measured and analyzed by cone-beam CT (cone-beam computed tomography, CBCT) , in order to provide some information during surgical procedures in interforaminal regions. METHODS: Two hundred and eighty-seven Chinese Han patients who visited Stomatological Department of Xiangya Hospital were included in this study. Patients were scanned by cone-beam computed tomography, and the information of interforaminal region was collected from the images. SPSS17.0 software package was used for statistical analysis. RESULTS: The mental foramen was usually apical to the second premolar or between the premolars(60.80% and 30.14%, respectively). The accessory mental foramina was observed in 4.88% of the mandibles, most of which were located under the second premolar, superiorly and distally to the mental foramen. The anterior loop was identified in 89.20% of cases with the mean length being (1.99±1.39) mm. The distance from the apex of the second mandibular premolar to the anterior loop or the inferior alveolar nerve was (5.21±2.50) mm. CONCLUSIONS: Accessory mental foramina is present in some mandibles. The anterior loop is highly prevalent in Chinese Han people, and its length is highly variable.
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    Expression and correlation of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma
    WU Ning, LU Ying, LIANG Ji-zong
    2016, 25 (6):  720-724. 
    Abstract ( 163 )   HTML ( 2 )   PDF (835KB) ( 172 )  
    PURPOSE: To detect the expression of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma (OSCC) and explore their correlation with clinicopathologic parameters. METHODS: Protein levels of survivin and hsa-miR-542-3p were measured in 30 OSCC patients and SCC-15 cell line. Plasma hsa-miR-542-3p level was analyzed in 30 OSCC patients and 30 normal controls. Statistical analysis was performed with SPSS 17.0 software package. RESULTS: In OSCC samples, survivin was increased while hsa-miR-542-3p was decreased. There was a negative correlation between survivin and hsa-miR-542-3p. In SCC-15 cell line, inhibitor and mimic of hsa-miR-542-3p could regulate survivin expression. Plasma level of hsa-miR-542-3p was decreased in OSCC patients. The potential diagnostic performance of plasma hsa-miR-542-3p for OSCC patients was evaluated by ROC curve analysis and AUC was 0.82. CONCLUSIONS: Down-regulation of hsa-miR-542-3p correlates with increasing of survivin expression in OSCC. Plasma hsa-miR-542-3p is a potential diagnostic biomarker for OSCC.
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    Changes of hard tissues in patients with functional and skeletal Angel Class Ⅲ malocclusion after FRⅢ appliance treatment
    ZHANG Nan, WANG Sun, ZHENG Wei, CHEN Min, SHEN Yu-feng, YAN Hong-juan
    2016, 25 (6):  725-728. 
    Abstract ( 230 )   HTML ( 2 )   PDF (1311KB) ( 215 )  
    PURPOSE: To compare changes of hard tissues before and after treatment of functional and skeletal Angle Class Ⅲ malocclusion with FRⅢ appliance. METHODS: Thirty patients with functional and skeletal Angel Class Ⅲ malocclusion were selected and treated with FRⅢ appliance. SPSS 22.0 software package was performed for statistical analysis. RESULTS: After treatment, there was no significant difference in SNA, S-Ptm, ANS-Ptm and Go-Po (P>0.05). ANB, ANS-Me, ANS-Me /N-Me were increased significantly (P<0.01). SNB, L1-NB, L1-MP were decreased significantly (P<0.05). MP-FH, Y axis, U1-NA, U1-NA (mm), U1-SN were increased significantly (P<0.05). CONCLUSIONS: After FRⅢ appliance treatment of Angel Class Ⅲ malocclusion, the position of mandible may rotate clockwise downward and backward, with retrusion of the lower incisors and protrusion of the upper incisors.
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    Expressions and clinical significance of TGF-βRⅡ and NF-κB in oral squamous cell carcinoma
    JIANG Li-Lan, ZHAO Ya-Jun, LI Yong
    2016, 25 (6):  729-733. 
    Abstract ( 187 )   HTML ( 4 )   PDF (892KB) ( 216 )  
    PURPOSE: To investigate the expression of nuclear factor κappa-B (NF-κB) and transforming growth factor β receptor Ⅱ (TGF-βRⅡ) and their clinical pathological significance in oral squamous cell carcinoma(OSCC). METHODS: The expression of NF-κB and TGF-βRⅡ in 60 OSCC samples, 20 adjacent tissues, 29 metastatic lymph nodes and 10 non-metastatic lymph nodes were detected by immunohistological method. Statistical analysis was performed with SPSS 20.0 software package. RESULTS: The expression of NF-κB in OSCC and metastatic lymph nodes was significantly higher than that in the adjacent tissues and non-metastatic lymph nodes, respectively (P<0.05). However, the expression of TGF-βRⅡ in OSCC and metastatic lymph nodes was significantly lower than that in the adjacent tissues and non-metastatic lymph nodes, respectively (P<0.05). NF-κB was negatively correlated with TGF-βRⅡ. The expression of NF-κB in patients with lower differentiation, lymphatic metastasis and higher grade were significantly higher than in patients with higher differentiation, lower grade and without lymphatic metastasis (P<0.05), respectively. NF-κB was a predicator of OSCC. CONCLUSIONS: NF-κB is negatively correlated with TGF-βRⅡ.NF-κB and TGF-βRⅡ are associated with the progression of OSCC. NF-κB may promote angiogenesis through down-regulating the expression of TGF-βRⅡ, which promotes progression of OSCC.
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    Effects of double transparent pressure diaphragm transfer tray on indirect bonding
    HUANG Xiao-hong, XU Liang, LIN Shan
    2016, 25 (6):  734-737. 
    Abstract ( 187 )   HTML ( 4 )   PDF (894KB) ( 277 )  
    PURPOSE: To compare the time-consuming and bonding effectiveness of full dental arch and segmented dental arch, when double transparent pressure diaphragm technology was used for indirect bracket bonding. METHODS: Forty-five orthodontic cases were selected, and classified into 3 groups according to different bonding methods and arches. There were 15 cases in each group, a total of 270 brackets and 120 buccal tubes were used. Patients in group A and B received double transparent pressure diaphragm technology to bond brackets indirectly. Among them, full dental arch tray was applied in group A, segmented dental arch tray was applied in group B; the brackets was bonded directly in group C. High posterior teeth pad did not affect the mandibular bracket during occlusion. The amount of time to bond brackets in group A and B (started from brackets bonding to the end of light-cure) was recorded as T1, the time of making arches was recorded as T2 (started from pressed film to the end of the arches made) and the average chair-side time of group A, B and C (started from acid etching in the mouth until all brackets are finished bonding and curing). Time-consuming of each stage in group A, B, immediate bracket failure rate and immediate buccal tube failure rate in group A, B, C were compared. SPSS 22.0 software package was used for statistical analysis. RESULTS: There was no significant difference in T1 and T2 between group A and B (P>0.05). T2 in group A was significantly less than in group B (P<0.05). Immediate buccal tube and braces failure rate in group A was significantly greater than in group B and C. CONCLUSIONS: Using double transparent pressure diaphragm technology to bond bracket indirectly is convenient and simple. The segmented dental arch is more time-consuming compared to full dental arch. However, the immediate bracket failure rate is lower.
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    Study of condyle movement in Angle Class Ⅲ malocclusion with temporomandibular dysfunctions
    CHANG Zhong -fu, CHEN Jie
    2016, 25 (6):  738-743. 
    Abstract ( 206 )   HTML ( 1 )   PDF (1418KB) ( 225 )  
    PURPOSE: Subjects with normal occlusion, Angle Class Ⅲ malocclusion and Angle Class Ⅲ malocclusion with temporomandibular dysfunctions(TMD) were included, and their protraction and retraction, opening and closing mouth were recorded and compared to determine the condyle movement characteristics of Angle Class Ⅲ malocclusion patients with TMD. METHODS: Thirty cases of Angle Class Ⅲ malocclusion with TMD (experimental group), 30 cases of simple Angle Class Ⅲ malocclusion (control group 1) and 30 cases of normal occlusion (control group 2) were chosen, the condyle movement of mouth opening and closing, protraction and retraction were recorded and analyzed by computer aided axiography (CADIAX Ⅲ) , the measurements were compared and qualitative analysis was performed. SPSS 10.0 software package was used for statistical analysis. RESULTS: The indexes of opening and closing mouth, forward, backward and lateral movement were higher in experimental group and control group 1 than in the control group 2, especially in the vertical direction. The maximum variation was on the Y axis between the experimental group and the control group 2, the lateral movement of working side was significantly higher than that of the normal group, while the movement ranges at other directions were significantly smaller than that of control group 2, especially in opening and closing mouth horizontally upward and the maximum space displacement at right, upward direction (P<0.01). CONCLUSIONS: The tracking of condyle movements has its own characteristics in three dimensions in the experimental group ,compared with control group 1 and 2.
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    Chinese expert consensus on the use of topical timolol maleate treatment of infantile hemangiomas
    ZHENG Jia-wei,WANG Xu-kai, JIANG Cheng-hong, QIN Zhong-ping, FAN Xin-dong, LI Ka, YANG Yao-w, HUO Ran, LIU Shao-hua,ZHAO Ji-hong, WANG Xiao-yong, ZHOU De-kai, LEI Shao-rong
    2016, 25 (6):  744-747. 
    Abstract ( 293 )   HTML ( 4 )   PDF (524KB) ( 451 )  
    [Summary] Non-selective β-blocker propranolol has been proved by FDA as the first-line agent for infantile hemangioma (IH) with dramatic response. To reduce the side effects caused by systemic administration of propranolol, timolol maleate treatment has been increasingly used as an alternative to systemic β-blockers and watchful waiting for many IH patients in recent years. However, the appropriate indications, drug dosage, dosing regimen, time for initiation, optimal duration, monitoring for side effects still remains controversial. To standardize the use of topical timolol in treating IH, avoid overtreatment or under-treatment, as well as minimize complications, a Chinese expert consensus on the use of topical timolol treatment of IH has been approved and written by a multidisciplinary experts group based on an up-to-date literature review and repeated discussion, which can be used to reduce inappropriate variations in clinical practice and to promote the delivery of high quality, evidence-based health care for IH patients.
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    Diameter measurements of the smoke particles produced by high frequency electrotome during prosthetic surgery and its protection
    TANG Jun, NIE Rong-bing
    2016, 25 (6):  748-750. 
    Abstract ( 250 )   HTML ( 1 )   PDF (593KB) ( 211 )  
    PURPOSE: To measure the particle size of surgical smoke produced by high frequency electrotome during prosthetic treatment. METHODS: The smoking machine was turned on when using high frequency electrotome simultaneously. Changes in the PM2.5 and PM10 concentration were measured by air quality detector. The sponge filter used in the smoking machine for 2 weeks was used as the experimental group with the intact one served as the control. The diameter and distribution of the smoke particles were observed under electric microscope scanning (SEM), and the difference between 2 groups was analyzed using SPSS 18.0 software package. RESULTS: Both PM 2.5 and PM 10 concentrations increased dramatically by the surgical smoke. SEM results demonstrated that the mean diameter of smoke particles was approximately 3.3 μm and most of them were smaller than 10 μm. CONCLUSIONS: The diameter of surgical smoke particles was similar to PM2.5 size, which is harmful to human health. Address of individual awareness and enhancing safety control are recommended.
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    Applications of minimally invasive bone splitting technique in cases with missing maxillary anterior teeth and obvious labial alveolar bone depression for dental implantation
    BAO Ji-bo, XIAO Xu-hui, XIE Zhi-gang, XIE Liang-kun, LI Zi-liang
    2016, 25 (6):  751-754. 
    Abstract ( 485 )   HTML ( 2 )   PDF (1373KB) ( 194 )  
    PURPOSE: The purpose of this study was to introduce a minimally invasive bone splitting technique which is suitable for cases with missing anterior teeth and obvious depression of alveolar bone in labial side, and to evaluate its clinical results. METHODS: Minimally invasive bone splitting technique was used in 8 healthy adults with bone defects in the aesthetic zone. The labial alveolar bone incisions were confined around the bone defects which were smaller than traditional incisions. The other procedures were the same as conventional bone splitting technique. Cone-beam CT (CBCT) for missing anterior teeth was taken before surgery, after the surgery and 6 months after surgery and alveolar bone height and width were recorded with landmark identification designed by ourselves in this study. The data were analyzed with SPSS 21.0 software package for paired t test. RESULTS: Paired t test indicated that after surgery and six months after surgery, the labial bone defect was significant improved (P<0.05), but the height of the alveolar ridge bone didn't increase significantly (P>0.05) while the width of the alveolar ridge bone significant improved (P>0.05). CONCLUSIONS: This minimally invasive technique can achieve good clinical results for not only intact labial alveolar ridge bones but also good bone grafts, which is beneficial to implantation and prosthetic aesthetics. The long-term outcome needs to be observed.
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    Clinical observation of assisted occlusion therapy for repositioning temporomandibular joint discs
    YIN Zhi-guo, ZOU Yin, GONG Zhong-jian
    2016, 25 (6):  755-757. 
    Abstract ( 415 )   HTML ( 14 )   PDF (636KB) ( 252 )  
    PURPOSE: To investigate the occurrence of postoperative malocclusion of patients with temporomandibular joint disc repositioning and the necessity of postoperative orthodontic treatment. METHODS: One hundred and eight patients who received temporomandibular joint disc repositioning from 2010.10 to 2015.10 were selected in this study. The patients' occlusion was recorded preoperatively and postoperatively. All patients received functional appliance or orthodontic treatment after surgery. The occlusion and the relative position of the articular disc and condyle were evaluated at regular follow-up. RESULTS: Postoperative malocclusion occurred in all patients. The use of functional appliance for 3-6 months may decrease the proportion of malocclusion. After orthodontic treatment, all patients had a complete recovery of malocclusion and remained good articular disc and condyle relationship during long term follow-up. CONCLUSIONS: Postoperatively malocclusion may occur after temporomandibular joint disc repositioning, and the use of functional appliance and orthodontic treatment are strongly recommended to retain good articular disc and condyle relationship.
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    Efficacy of semiconductor laser therapy combined with botulinum toxin A type injection in treatment of temporomandibular disorders
    JIANG Mei-yuan, YOU Qing-ling
    2016, 25 (6):  758-761. 
    Abstract ( 221 )   HTML ( 1 )   PDF (474KB) ( 209 )  
    PURPOSE: To observe the efficacy of semiconductor laser therapy in combination with botulinum toxin A type injection in treating temporomandibular disorders and the influence on serum levels of interleukin(IL)-1 and tumor necrosis factor(TNF)-α. METHODS: Ninety patients with temporomandibular disorders were selected and randomly divided into control group and treatment group with 45 cases in each group. Patients in the control group received semiconductor laser therapy,once per day and 1 course for 1 week. Patients in the treatment group was given semiconductor laser therapy in combination with botulinum toxin A type. 50 U botulinum toxin A type were injected into the masseteric and temporal muscles with 5 injections at different sites in each side. The treatment course of patients was 2 weeks. Fricton scale indexes, pain index, and efficacy were compared between the two groups. Serum levels of IL-1 and TNF-α were detected in both groups. SPSS19.0 software package was used for data analysis. RESULTS: After treatment, PI, DI and CMI in the treatment group were significantly lower than the control group (P<0.01). After 1 and 2 weeks of treatment, VAS score of the treatment group was significantly lower than the control group (P<0.01). The total efficacy rate of the treatment group was 93.33%,which was significantly higher than control group (66.67%, P<0.05). Serum levels of IL-1 and TNF-α of the treatment group were significantly lower than the control group (P<0.01). CONCLUSIONS: The efficacy of semiconductor laser therapy in combination with botulinum toxin A type injection in treating temporomandibular disorders is significant,and may be related to decreased serum levels of IL-1 and TNF-α.
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    Applications of 3D printing technology in teaching of oromaxillofacial head and neck surgical oncology
    RUAN Min, JI Tong, ZHANG Chen-ping
    2016, 25 (6):  762-765. 
    Abstract ( 243 )   HTML ( 1 )   PDF (624KB) ( 252 )  
    [Summary] With the increasing maturation of 3D printing technology, as well as its application in various industries, investigation of 3D printing technology into clinic medical education becomes an important task of the current medical education. The teaching content of oromaxillofacial head and neck surgical oncology is complicated and diverse, making lower understanding/memorizing efficiency and insufficient skill training. To overcome the disadvantage of traditional teaching method, it is necessary to introduce 3D printing technique into teaching of oromaxillofacial head and neck surgical oncology, in order to improve the teaching quality and problem solving capabilities, and finally promote cultivation of skilled and innovative talents.
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    Treatments of second mesiobuccal canals in maxillary permanent molar: a report of 2 cases WEI Yan-pei, FAN Hong-xia, CHEN Liang, ZHANG Xue, YU Jing-tao. (Department of Endodontics, China Medical University School of Stomatology. Shenyang 110002, Liaoning Province, China)
    WEI Yan-pei, FAN Hong-xia, CHEN Liang, ZHANG Xue, YU Jing-tao.
    2016, 25 (6):  766-768. 
    Abstract ( 592 )   HTML ( 1 )   PDF (633KB) ( 208 )  
    [Summary] The incidence of MB2in maxillary permanent molar is fairly high, but the clinical detection rate is lower. Generally, failed endodontic treatment of maxillary molars is caused by undetection of the MB2. This paper reported2cases of missing MB2, summarized the reasons for undetection, the methods of localization of MB2
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