Influence of different ultrasonic irrigation solutions after root canal preparation with ProTaper by machine on micro-hardness of root canal dentin

Abstract

Abstract: PURPOSE: To develope the influence of different ultrasonic irrigations after root canal preparation with nickel titanium ProTaper on micro-hardness of root canal dentin. METHODS: Sixty of maxillary anterior teeth with single-canal were collected and randomly divided into 6 groups. Group A was control group, group B was prepared to F3 with nickel titanium ProTaper by machine, group C was ultrasonic irrigated with 3% hydrogen peroxide solution for 1 minute after preparation, group D was ultrasonic irrigated with koutai mouthwash for 1 minute after preparation, group E was ultrasonic irrigated with 17% EDTA solution for 1 minute after preparation, group F was ultrasonic irrigated with distilled water for 1 minute after preparation. The roots were then sectioned horizontally into 3 parts,split longitudinally into halves and examined under a micro Vickers hardness test machine. The data was analyzed by one-way ANOVA and t test with SPSS 17.0 software package. RESULTS: The micro-hardness of group A was(52.66±1.64) HV,(52.08±1.53）HV and（51.47±2.53）HV. There was no significant difference in all parts of the root canal in group A(P>0.05). The micro-hardness of the apical third of root canal was lower than that of the cervical and middle of root canal in the other groups (P<0.05). In the cervical and middle third of the root canals, the micro-hardness of group E was(44.65±1.33) HV and(42.55±1.12) HV, and there were statistical significances between group E and the other groups（P<0.05）.In the apical third of root canal,the micro-hardness of group E was (37.82±1.60）HV, and group C was (44.14±1.73) HV, both of the comparative differences with other groups were statistically significant (P<0.05). There was no significant difference among group B, group D and group F (P>0.05). CONCLUSIONS: Root canal preparation to F3 with nickel titanium ProTaper by machine can make the micro-hardness of the apical third of root canal decrease. Ultrasonic irrigation with 17% EDTA solution for 1 minute can make the micro-hardness of the root canal decrease ultrasonic irrigation with. Ultrasonic irrigation with 3% hydrogen peroxide can make the micro-hardness of the apical third of root canal decrease. Ultrasonic irrigation with Koutai mouthwash and distilled water for 1 minute have no influence on the micro-hardness of root canal.

Key words: Nickel-titanium ProTaper, Micro-hardness, Ethylenediamine tetraacetic acid, ChlorhexidineShanghai J Stomatol, 2015, 24(4):451-454.

CLC Number:

GUO Jiang-li, ZHANG Yan, ZHEN Lei. Influence of different ultrasonic irrigation solutions after root canal preparation with ProTaper by machine on micro-hardness of root canal dentin[J]. Shanghai Journal of Stomatology, 2015, 24(4): 451-454.

References

[1] Pascon FM, Kantovitz KR, Gaspar JF, et al. Effects of chemical agents on physical properties and structure of primary pulp chamber dentin[J].Microsc Res,Tech,2014,11(1):52-56.
[2] Vârlan C, Dimitriu B, Vârlan V, et al. Current opinions concerning the restoration of endodontically treated teeth: basic principles[J]. J Med Life, 2009, 2(2): 165-172.
[3] Saleh AA, Ettman WM. Effect of endodontic irrigation solutions on micro-hardness of root canal dentine[J]. J Dent, 1999,27(1): 43-46.
[4] 刘会梅, 牛玉梅, 徐俊杰, 等. 根管锥度和循环疲劳对牙根力学性能的影响 [J]. 口腔医学研究, 2007, 23(2): 191-193.
[5] Kim HC, Lee MH, Yum J, et al. Potential relationship between design of nickel-titanium rotary instruments and vertical root fracture [J]. J Endod,2010,36(7):1195-1199.
[6] Shemesh H, Wesselink PR, Wu MK. Incidence of dentinal defects after root canal filling procedures[J]. Int Endod J, 2010, 43(11): 995-1000.
[7] Hülsmann M, Heckendorff M, Lennon A. Chelating agents in root canal treatment: mode of action and indications for their use [J]. Int Endod J, 2003, 36(12): 810-830.
[8] Cruz-Filho AM, Sousa-Neto MD, Savioli RN, et al. Effect of chelating solutions on the microhardness of root canal lumen dentin [J]. J Endod, 2011, 37(3): 358-362.
[9] Calt S, Serper A. Time dependent effects of EDTA on dentin structures [J]. J Endod, 2002, 28(1): 17-19.
[10] Souza M, Cecchin D, Farina AP, et al. Evaluation of chlorhexidine substantivity on human dentin: a chemical analysis[J]. J Endod, 2012, 38(9): 1249-1252.
[11] 黄辉. 口泰用于根管超声冲洗抗菌效果的研究 [J]. 口腔医学研究, 2009, 20(4): 420-422.
[12] 贾剑巍, 赵露露. 口泰冲洗液对根管预备抗菌效果的影响 [J]. 中国医药指南, 2012, 10(24): 227.
[13] 黄辉, 刘漪, 罗丽华. 3种冲洗液用于超声根管预备抗菌效果的对比研究[J]. 现代口腔医学杂志, 2007, 21(2): 132-134.
[14] Yamashita JC, Tanomaru Filho M, Leonardo MR, et al. Scanning electron microscopic study of the cleaning ability of chlorhexidine as a root- canal irrigant [J]. Int Endod J, 2003, 36(6): 391-394.
[15] 姚红. 不同冲洗液及冲洗时间对牙本质微硬度的影响 [J]. 口腔医学研究, 2007, 23(6):688-690.
[16] Sen BH, Wesselink PR, Turkum M. The smear layer:a phenomenon in root canal therapy [J]. Int Endod J, 1995, 28(3): 141-148.