Evaluation of the effect for ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans

Abstract

Abstract: PURPOSE: To analyze the effect of ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans in vitro. METHODS: By using electroporation, UA159-FR was transformed and combined with targeted site of ffh gene sequence, and the best piece of siRNA for fluoride resistant Streptococcus mutans was screened. In different values of pH of BHI, they were cultured for 24 hours with UA159-FR respectively, and then centrifugated to determine the pH and OD_600. SPSS17.0 software package was used to analyze the data. RESULTS: The aciduricity of UA159-FR had significant differences compared with ffh gene silencing for UA159-FR in ΔpH (P<0.05), and the former was higher than the latter. At pH=3.5~5.0, P<0.01; at pH=5.5~7.5, P<0.05. Significant differences were noted in OD₆₀₀ and their growth tendency were similar. CONCLUSIONS: The aciduricity of fluoride resistant Streptococcus mutans has significant effect when the ffh gene is silenced.

Key words: ffh, Gene silencing, Fluoride resistant Streptococcus mutans, Aciduricity Shanghai J Stomatol,2015, ,24(4):395?399.

CLC Number:

R783.6

LI Zhen-ling, ZHANG Zhi-min, LI Peng-lian, ZHANG Tong-fei, LI Tian-bo, LIU Fu-ping. Evaluation of the effect for ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans[J]. Shanghai Journal of Stomatology, 2015, 24(4): 395-399.

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