Shanghai Journal of Stomatology ›› 2014, Vol. 23 ›› Issue (5): 524-530.

• Basic Study • Previous Articles     Next Articles

The effects of ADAM28 AS-ODN on biological function of human gingival fibroblasts

ZHAO Zheng, WEI Li, Li Lu-jia, YE Yong, HUANG Zheng-nan, YANG Hai-qing   

  1. Department of Stomatology, the 401 Hospital of Chinese PLA. Qingdao 266071, Shandong Province, China)
  • Received:2014-05-12 Revised:2014-07-09 Online:2014-10-20 Published:2015-02-04
  • Supported by:
    ; Supported by Chinese Post-Doctoral Special Science Foundation (201003774)

Abstract: PURPOSE: To study the effects of a disintegrin and metalloproteinase 28 (ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human gingival fibroblasts (HGFs), and analyze the possible mechanism. METHODS: Cell culture, gene transfection, MTT chromatometry, enzyme dynamics, and flow cytometry (FCM) techniques were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HGFs after transfected into HGFs. The statistical differences were evaluated by SNK test with SPSS 16.0 software package. RESULTS: In ADAM28 AS-ODN group, the proliferation activity of HGFs decreased significantly. Cell percentage in S phase in AS-ODN group was notably lower than that of S-ODN and untransfected groups, and cell percentage in G2+M phase was remarkably lower than that of untransfected group. Cell proliferation index(PI=S+G2M)in AS-ODN group was significantly lower than that of the other two groups. There was a significant difference between the groups. In AS-ODN group, alkaline phosphatase (ALP) secretion activity and percentage of apoptotic cell notably increased. CONCLUSIONS: ADAM28 AS-ODN could inhibit HGFs proliferation significantly and influence the changes of cell cycle, promote HGFs differentiation and induce HGFs apoptosis remarkably.

Key words: ADAM28, AS-ODN, HGFs