Shanghai Journal of Stomatology ›› 2015, Vol. 24 ›› Issue (4): 385-389.

• Basic study •     Next Articles

Effect of beryllium on the morphology and chemical elements of cell membrane of Porphyromonas gingivalis

LI Wei-hong1, HUANG Rui2, LIN Hua2, LI Qing-yan2, ZHENG Xin-ying2, LV Qiao2, GAO Ning2   

  1. 1.The Second Affiliated Hospital of Zhejiang University, School of Medicine. Hangzhou 310052, Zhejiang Province; 2.Department of Prosthodontics, West China Stomatology College, Sichuan University. Chengdu 610041, Sichuan Province, China
  • Received:2014-01-14 Online:2015-08-20 Published:2015-09-10
  • Supported by:
    Supported by National Natural Science Foundation of China (81371182), Natural Science Foundation of Zhejiang Province (13H140005 and Y2110400) and Science Foundation of of Zhejiang Provincial Department of Health (2013KYA124)

Abstract: PURPOSE: To evaluate the effect of beryllium (Be2+) on the morphology and chemical elements on cell membrane of Porphyromonas gingivalisP. gingivalis), thus to explore the microbiologic mechanisms of periodontal diseases. METHODS: P. gingivalis was put into the culture with different Be2+ concentrations and anaerobically cultured for 24 hours. The morphologic change of P. gingivalis was observed under microscope and scanning electronic microscope (SEM), and chemical elements of cell membrane were observed by X-ray energy dispersion spectrum (EDS). The data was statistically analyzed with SPSS13.0 software package. RESULTS: The morphology of P.gingivalis altered obviously at the concentration greater than 2.5 mg/L, which was manifested by the sharpness of border and depression on the surface. With the increased concentration of beryllium, the Na and Ca peak descended on the surface of P. gingivalis. CONCLUSIONS: Beryllium can interfere with the morphology of P. gingivalis, and lead to the changes of chemical elements on cell membrane of P. gingivalis, which may result in a disturbance in the microecologic balance of subgingival microbes and eventually contribute to periodontal diseases.

Key words: Beryllium, Dental alloy, Porphyromonas gingivalis, Oral microecologyShanghai J Stomatol, 2015, 24(4):385-389.

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