Expression pattern of Krüpple-like factor 5 in odontoblasts

doi:10.19439/j.sjos.2017.06.002

Shanghai Journal of Stomatology ›› 2017, Vol. 26 ›› Issue (6): 582-585.doi: 10.19439/j.sjos.2017.06.002

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Expression pattern of Krüpple-like factor 5 in odontoblasts

DU Jian-xin, HU Wei

Department of Stomatology, Zibo Central Hospital. Zibo 255000, Shandong Province, China

Received:2017-06-16 Revised:2017-08-07 Online:2017-12-20 Published:2018-01-09

Abstract

Abstract: PURPOSE: To investigate the expression of Krüpple-like factor 5 (KLF5) in human pulp-dentin complex and the function of KLF5 in differentiation and dentinogenesis of odontoblasts. METHODS: Immunohistochemistry staining was used to investigate the expression of KLF5 in human pulp-dentin complex and dental pulp cells. Western blot was used to analyze the expression patterns of KLF5 during dental pulp cell differentiation. Significant differences between groups were evaluated using one-way ANOVA and Tukey's test with SPSS17.0 software package. RESULTS: Immunohistochemical staining showed KLF5 was positively expressed in the odontoblasts and odontoblast-like cells, but not in dentin and predentin in both normal and carious teeth. Moreover, during mineralization of human dental pulp cells, KLF5 was primarily localized in the nuclei of dental pulp cells. Western blot showed KLF5 was significantly up-regulated during odontoblast differentiation. CONCLUSIONS: KLF5 may play an important role in odontoblast differentiation and dentin formation.

Key words: Krü, pple-like factor 5, Pulp-dentin complex, Odontoblast

CLC Number:

R781.3

DU Jian-xin, HU Wei. Expression pattern of Krüpple-like factor 5 in odontoblasts[J]. Shanghai Journal of Stomatology, 2017, 26(6): 582-585.

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Expression pattern of Krüpple-like factor 5 in odontoblasts

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