上海口腔医学

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (4): 355-359.doi: 10.19439/j.sjos.2021.04.004

• 论著 • 上一篇    下一篇

DKK1对脂多糖感染人牙髓细胞生物学行为的影响

徐方芳, 蒋备战   

  1. 同济大学口腔医学院和附属口腔医院 儿童口腔医学教研室,上海牙组织修复与再生工程技术研究中心,上海 200072
  • 收稿日期:2020-02-17 修回日期:2020-04-01 出版日期:2021-08-25 发布日期:2021-09-23
  • 通讯作者: 蒋备战,E-mail:jiangbeizhan@tongji.edu.cn
  • 作者简介:徐方芳（1994-）,女,硕士,住院医师,E-mail:1632345@tongji.edu.cn
  • 基金资助:
    上海市科学技术委员会医学引导项目（18411969500）; 上海市卫生健康委员会面上项目（201740223）

Effect of DKK1 on the biological behaviors of dental pulp cells exposed to lipopolysaccharide

XU Fang-fang, JIANG Bei-zhan   

  1. Department of Pediatric Dentistry, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration. Shanghai 200072, China
  • Received:2020-02-17 Revised:2020-04-01 Online:2021-08-25 Published:2021-09-23

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摘要： 目的: 研究DKK1对脂多糖（lipopolysaccharide,LPS）感染人牙髓细胞生物学行为的影响。方法: 使用改良酶组织块法分离培养人牙髓细胞并通过免疫荧光染色鉴定细胞来源;通过细胞计数试剂盒（CCK-8）、Transwell实验、茜素红染色、荧光定量PCR等实验检测DKK1对LPS感染人牙髓细胞的生物学行为的变化。采用SPSS 20.0软件包对数据进行统计学分析。结果: 免疫荧光结果显示分离的细胞来源与间充质细胞一致;CCK-8结果显示DKK1对LPS感染下的牙髓细胞增殖并无显著影响;Transwell实验结果显示,DKK1对LPS感染下的牙髓细胞迁移具有促进作用;茜素红与荧光定量PCR结果显示,DKK1可显著促进LPS感染下牙髓细胞的细胞分化。结论: DKK1对LPS感染下的人牙髓细胞的迁移和分化具有一定的促进作用,推测其与Wnt/β-catenin信号通路的抑制相关。

关键词: 牙髓细胞, 脂多糖, Wnt/β-catenin信号通路, 细胞迁移, 细胞分化

Abstract: PURPOSE: To detect the effect of DKK1 on biological behaviors of human dental pulp cells exposed to lipopolysaccharide (LPS). METHODS: The dental pulp cells were isolated and cultured by modified enzyme-tissue block method and identified by immunofluorescence staining. The effect of DKK1 on proliferation and migration of human dental pulp cells exposed to LPS were measured by cell counting kit (CCK-8) and Transwell assay. Meanwhile, the effect of DKK1 on differentiation of human dental cells exposed to LPS were studied by alizarin red staining and real-time PCR experiment, statistical analysis was performed using SPSS 20.0 software package. RESULTS: The results of immunofluorescence showed that the cultured cells were in consistent with the mesenchymal stem cells. The result of CCK-8 indicated that DKK1 had no significant effect on proliferation of dental pulp cells exposed to LPS; The result of transwell assay showed that DKK1 significantly promoted the cell migration of dental pulp cells exposed to LPS. The results of Alizarin red staining and real-time PCR revealed that DKK1 could promote cytodifferentiation of dental pulp cells exposed to LPS. CONCLUSIONS: DKK1 promotes the ability of cell migration and cytodifferentiation of LPS treated dental pulp cells, which may be resulted from inhibition of Wnt/β-catenin pathway.

Key words: Dental pulp cells, Lipopolysaccharide, Wnt/β-catenin signaling pathway, Cell migration, Cytodifferentiation

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