兔BMSCs复合β-TCP修复牙槽骨缺损后牙移动时机的探讨

doi:10.19439/j.sjos.2019.03.002

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (3): 231-236.doi: 10.19439/j.sjos.2019.03.002

兔BMSCs复合β-TCP修复牙槽骨缺损后牙移动时机的探讨

张飞飞¹, 包世婕¹, 叶盛佳¹, 魏斌^2,*, 宫耀^3,*

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011;
2.口腔第一门诊国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011;
3.口腔正畸科,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2019-01-29 修回日期:2019-03-05 出版日期:2019-06-25 发布日期:2019-08-09
通讯作者: 魏斌,E-mail: weibin0328@hotmail.com;宫耀,E-mail: gongy0328@126.com。^*共同通信作者
作者简介:张飞飞（1982-）,男,硕士研究生,E-mail: 544789058@qq.com
基金资助:
上海市科学技术委员会科研计划项目（16140902202）

Study of the timing of tooth movement after repair of alveolar bone defects by rabbit BMSCs combined with beta-TCP

ZHANG Fei-fei¹, BAO Shi-jie¹, YE Sheng-jia¹, WEI Bin², GONG Yao³

1.Department of Prosthodontics Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011,China;
2.Special Dental Consultation Clinic Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011,China;
3.Department of Orthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011,China

Received:2019-01-29 Revised:2019-03-05 Online:2019-06-25 Published:2019-08-09

摘要/Abstract

摘要： 目的探讨兔骨髓间充质干细胞（bone marrow mesenchymal stem cells, BMSCs）复合β磷酸三钙（β tricalcium phosphate,β-TCP）构建的组织工程骨修复牙槽骨缺损区内开展正畸牙移动的最佳介入时机。方法选择40只新西兰大白兔,拔除右侧下颌第一磨牙,扩大牙槽窝,形成6 mm×4 mm×8 mm的缺损,植入构建好的兔BMSCs复合β-TCP组织工程骨（实验侧）,左侧单纯拔牙（对照侧）。分别在术后2、4、8、12周进行下颌第二磨牙近中移动,加力4周后取标本。测量下颌第二磨牙的移动距离,H-E染色观察第二磨牙近远中牙周组织变化。TRAP染色,选取压力侧牙周组织进行破骨细胞计数。BMP-2免疫组织化学染色观察张力侧牙周组织平均吸光度值。采用SAS 8.0软件包对数据进行统计学分析。结果 2周、4周组实验侧牙移动距离、TRAP阳性细胞计数、BMP-2吸光度值均显著小于对照侧;8周组、12周组实验侧与对照侧的各项指标无统计学差异。结论兔BMSCs复合β-TCP修复兔下颌牙槽骨缺损8周后是进行正畸牙移动的适宜时机。

关键词: 牙槽骨缺损, 骨髓间充质干细胞, β磷酸三钙, 牙移动

Abstract: PURPOSE: To investigate the effect of tooth movement at different time after repair of alveolar bone defects with tissue engineering bone constructed by rabbit bone marrow mesenchymal stem cells (BMSCs) and beta-tricalcium phosphate (β-TCP). Methods: Alveolar bone defect (6 mm×4 mm×8 mm) was made on the right side of 40 New Zealand rabbits, which was filled with tissue engineering bone constructed by BMSCs and β-TCP as experimental sides. Tooth extraction on the other side was performed as control. The mandibular second molars in both sides were moved mesially 2, 4, 8 and 12 weeks after surgery. The specimens were taken 4 weeks after exertion. The distance of mandibular second molar moved was measured. The periodontal tissues were observed after H-E staining. TRAP staining was performed and osteoclasts were counted in the periodontal tissues on the pressure side. BMP-2 immunohistochemical staining was used to observe the average optical density of periodontal tissue on tension side. The results were analyzed with SAS 8.0 software package. Results: The distance of tooth movement, the amount of TRAP positive cells and the optical density of BMP-2 in the experimental side of the 2-week and 4-week groups were all significantly lower than those in the control side, while there was no significant difference between the 8-week and 12-week groups.Conclusions: Eight weeks after repair of alveolar bone defect with rabbit BMSCs and β-TCP is an appropriate time for orthodontic tooth movement.

Key words: Alveolar defect, Bone marrow mesenchymal stem cells, Beta-tricalcium phosphate, Tooth movement

中图分类号:

R783.5

张飞飞, 包世婕, 叶盛佳, 魏斌, 宫耀. 兔BMSCs复合β-TCP修复牙槽骨缺损后牙移动时机的探讨[J]. 上海口腔医学, 2019, 28(3): 231-236.

ZHANG Fei-fei, BAO Shi-jie, YE Sheng-jia, WEI Bin, GONG Yao. Study of the timing of tooth movement after repair of alveolar bone defects by rabbit BMSCs combined with beta-TCP[J]. Shanghai Journal of Stomatology, 2019, 28(3): 231-236.

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兔BMSCs复合β-TCP修复牙槽骨缺损后牙移动时机的探讨

Study of the timing of tooth movement after repair of alveolar bone defects by rabbit BMSCs combined with beta-TCP

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