沙利霉素对舌鳞癌细胞体外增殖、侵袭和迁移能力的影响

doi:10.19439/j.sjos.2019.01.004

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (1): 20-24.doi: 10.19439/j.sjos.2019.01.004

沙利霉素对舌鳞癌细胞体外增殖、侵袭和迁移能力的影响

陈洁¹, 季平^1,2,3, 苏雷震¹, 李显¹

1.重庆医科大学附属口腔医院口腔颌面外科,重庆 401147;
2.口腔疾病与生物医学重庆市重点实验室,重庆 401147;
3.重庆市高校市级口腔生物医学工程重点实验室,重庆 401147

收稿日期:2018-06-20 修回日期:2018-09-05 出版日期:2019-02-25 发布日期:2019-04-12
通讯作者: 季平,E-mail：jiping1962@hotmail.com
作者简介:陈洁（1993-）,女,硕士,住院医师,E-mail：877239376@qq.com
基金资助:
2016年重庆高校创新团队建设计划资助项目（CXTDG201602006）; 重庆市高校市级口腔生物医学工程重点实验室资助项目; 2017重庆市卫生计生委医学科研项目（2017MSXM075）; 重庆市科委社会民生一般项目（cstc2015shmszx10008）; 重庆市渝北区科委重点项目[2015（社）01号]

Effects of salinomycin on proliferative, migratory and invasive property of tongue squamous cell carcinoma cells in vitro

CHEN Jie¹, JI Ping^1,2,3, SU Lei-zhen¹, LI Xian¹

1.Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University. Chongqing 401147;
2.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences. Chongqing 401147;
3.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education. Chongqing 401147, China

Received:2018-06-20 Revised:2018-09-05 Online:2019-02-25 Published:2019-04-12

摘要/Abstract

摘要： 目的:探讨沙利霉素对口腔舌鳞癌细胞增殖、侵袭和迁移能力的影响,并初步分析其影响机制。方法:CCK8法检测沙利霉素和顺铂分别作用于CAL-27细胞和EA.hy926细胞后,对细胞增殖的影响;Transwell小室法检测沙利霉素对CAL-27细胞侵袭和迁移能力的作用;Western 免疫印迹检测沙利霉素对CAL-27细胞中E钙黏蛋白（E-cadherin）、波形蛋白（vimentin）和β连环蛋白（β-catenin）表达的影响。采用SPSS20.0 软件包对数据进行统计学分析。结果:沙利霉素抑制CAL-27细胞的增殖,呈时间、剂量依赖性,且其增殖抑制作用强于顺铂（P<0.05）;经过沙利霉素(4 μmol/L）处理过的CAL-27细胞,其侵袭和迁移能力均显著低于空白对照组(P<0.05);沙利霉素上调CAL-27细胞中E-cadherin蛋白的表达水平,并且下调vimentin和β-catenin蛋白的表达水平。结论:沙利霉素能够明显抑制CAL-27细胞的增殖能力,显著减弱CAL-27细胞的侵袭和迁移能力,其机制可能与抑制癌细胞发生上皮-间充质转化(epithelial-mesenchymal transitions,EMT)有关。

关键词: 沙利霉素, 舌, 鳞状细胞癌, 增殖, 侵袭, 上皮-间充质转化

Abstract: PURPOSE: To investigate the effects of salinomycin on proliferative, migratory and invasive properties of tongue squamous cell carcinoma cells, and to explore its possible mechanism. METHODS: CCK8 assay was used to detect the effect of salicamycin and cisplatin on proliferative abilities of CAL-27 cells and EA.hy926 cells. The invasive and migratory ability was detected by Transwell assay. The protein expressions of E-cadherin, vimentin and β-catenin was evaluated by Western blot. SPSS20.0 software package was used to analyze the data. RESULTS: Salicamycin can effectively inhibit proliferation of CAL-27 cells, and the inhibitive ability of salicamycin on the proliferation was stronger than that of cisplatin. CAL-27 cells were treated by salinomycin (4 μmol/L) before invasive and migratory abilities were examined. Compared with control group, the number of invasive and migratory cells in the salinomycin-treated group was significantly decreased (P<0.05). Western blot analysis showed that the protein expression of vimentin and β-catenin was significantly down-regulated. The expression of E-cadherin was significantly increased with the increase of salicamycin concentration. CONCLUSIONS: The proliferative, invasive and migratory ability of CAL-27 cells can be inhibited by salinomycin, which may be related to the inhibition of epithelial-mesenchymal transitions.

Key words: Salinomycin, Tongue, Squamous cell carcinoma, Proliferation, Invasion, Epithelial-mesenchymal transitions

中图分类号:

R739.8

陈洁, 季平, 苏雷震, 李显. 沙利霉素对舌鳞癌细胞体外增殖、侵袭和迁移能力的影响[J]. 上海口腔医学, 2019, 28(1): 20-24.

CHEN Jie, JI Ping, SU Lei-zhen, LI Xian. Effects of salinomycin on proliferative, migratory and invasive property of tongue squamous cell carcinoma cells in vitro[J]. Shanghai Journal of Stomatology, 2019, 28(1): 20-24.

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沙利霉素对舌鳞癌细胞体外增殖、侵袭和迁移能力的影响

Effects of salinomycin on proliferative, migratory and invasive property of tongue squamous cell carcinoma cells in vitro

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