上海口腔医学

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (1): 38-43.doi: 10.19439/j.sjos.2022.01.008

• 论著 • 上一篇    下一篇

大鼠同种异体骨髓间充质干细胞联合Bio-Oss骨粉-bFGF复合物促进拔牙窝愈合的micro-CT研究

江银华1,2, 商裕1,2, 邹多宏3, 陈露3, 费晨艳4, 梁凯5   

  1. 1.丽水学院,浙江 丽水 323000;
    2.丽水市人民医院, 浙江 丽水 323000;
    3.上海交通大学医学院附属第九人民医院·口腔医学院 口腔外科,上海 200011;
    4.浙江中医药大学,浙江 杭州 310053;
    5.南昌大学附属口腔医院,江西 南昌 330006
  • 收稿日期:2020-06-19 修回日期:2020-08-17 出版日期:2022-02-25 发布日期:2022-03-10
  • 通讯作者: 商裕, E-mail: 827875531@qq.com
  • 作者简介:江银华（1966-）, 男,教授,主任医师,E-mail：lsjyh1111@sina.com
  • 基金资助:
    丽水市医学重点建设项目（2016zdxk08）

Effect of rat allogeneic BMSCs-Bio-Oss-bFGF compound on tooth extraction healing: a micro-CT study

JIANG Yin-hua1,2, SHANG Yu1,2, ZOU Duo-hong3, CHEN Lu3, FEI Chen-yan4, LIANG Kai5   

  1. 1. Lishui University. Lishui 323000, Zhengjiang Province;
    2. Lishui People's Hospital. Lishui 323000, Zhengjiang Province;
    3. Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;
    4. Zhejiang Chinese Medical University. Hangzhou 310053, Zhejiang Province;
    5. Affiliated Stomatological Hospital of Nanchang University. Nanchang 330006, Jiangxi Province, China
  • Received:2020-06-19 Revised:2020-08-17 Online:2022-02-25 Published:2022-03-10

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摘要： 目的: 观察以Bio-Oss骨粉bFGF支架联合大鼠骨髓间充质干细胞（BMSCs）植入大鼠拔牙窝后在牙槽窝愈合过程中对牙槽骨微结构的影响。方法: 取3周龄SD大鼠股骨全骨髓,自然贴壁分离BMSCs。选取36只6周龄SD大鼠,拔除上颌后牙,立即植入不同材料。按植入材料分为4组,即复合物组植入BMSCs联合Bio-Oss骨粉bFGF复合物、骨粉组植入Bio-Oss骨粉、干细胞组植入BMSCs和空白组直接对位缝合拔牙创。植入后4、12、24周,取上颌骨标本做micro-CT扫描。采用GraphPad Prism 6.0软件包对数据进行双因素方差分析。结果: 植入第4周,骨密度（BMD）、骨小梁分离度（Tb.Sp）、骨小梁厚度（Tb.Th）、各向异性值（DA）及骨小梁数（Tb.N）,复合物组与骨粉组、干细胞组与空白对照组均无显著差异（P>0.05）。复合物组BMD在植入第12周显著高于骨粉组、干细胞组和空白对照组（P<0.05）;植入第24周显著高于骨粉组和空白对照组（P<0.05）。复合物组Tb.Th在第12周和第24周显著高于骨粉组（P<0.05）。第4、12、24周,复合物组与骨粉组、干细胞组及空白对照组DA均无显著差异（P>0.05）。复合物组Tb.Sp在植入后第24周显著小于骨粉组、干细胞组和空白对照组,复合物组Tb.N在植入后第24周显著高于干细胞组和空白对照组（P<0.05）。结论: 大鼠同种异体BMSCs联合Bio-Oss骨粉-bFGF复合物对大鼠拔牙窝的愈合有促进作用。

关键词: 骨髓间充质干细胞, Bio-Oss骨粉, bFGF, 拔牙窝, Micro-CT

Abstract: PRUPOSE: To investigate the effect of a compound of BMSCs-Bio-Oss-bFGF on microstructure of extraction sockets in rats. METHODS: Bone mesenchymal stem cells (BMSCs) were isolated from bone marrow of 3-week SD rats by adherent method. Maxillary posterior teeth of 36 6-week SD rats were extracted and materials were implanted into sockets according to grouping. The rats were divided into 4 groups: compound group with implanting BMSCs-Bio-Oss-bFGF compound, powder group with implanting Bio-Oss, BMSCs group with implanting BMSCs, and control group without implanting any materials. The sockets were scanned by micro-CT 4 weeks, 12 weeks and 24 weeks after implantation. Two-way ANOVA was used to assess whether there was significant difference between groups with GraphPad Prism 6.0 software package. RESULTS: There was no significant difference among groups in bone mineral density (BMD), trabecular separation(Tb.Sp), trabecular thickness(Tb.Th), degree of anisotropy(DA), and trabecular number(Tb.N) 4 weeks after implantation. By 12 weeks, BMD of compound group was significantly greater than those of BMSCs group, powder group and control group (P<0.05), and significantly greater than those of powder group and control group at 24 weeks (P<0.05). Tb.Th of compound group was significantly greater than that of BMSCs group at 12 and 24 weeks(P<0.05). DA had no significant difference among groups at 4, 12, and 24 weeks (P>0.05). Tb.Sp of compound group was significantly smaller than those of powder group, BMSCs group and control group at 24 weeks(P<0.05). Tb.N of compound group was significantly greater than those of BMSCs group and control group(P<0.05). CONCLUSIONS: The compound of rat allogeneic BMSCs-Bio-Oss-bFGF improves socket healing.

Key words: Bone marrow mesenchymal stem cells, Bio-Oss, bFGF, Socket, Micro-CT

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