Bmal1在经典Wnt通路调控间充质干细胞衰老中的作用

doi:10.19439/j.sjos.2020.06.008

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (6): 601-605.doi: 10.19439/j.sjos.2020.06.008

Bmal1在经典Wnt通路调控间充质干细胞衰老中的作用

叶翠, 吴海苗

上海市口腔病防治院口腔正畸科,上海 200031

收稿日期:2020-01-07 修回日期:2020-04-27 出版日期:2020-12-25 发布日期:2021-01-08
通讯作者: 吴海苗, E-mail: wuhaimiao0805@126.com
作者简介:叶翠（1981-）,女,博士,主治医师,E-mail: 19705797@qq.com
基金资助:
上海市自然科学基金（17ZR1424900）; 上海市卫生健康委员会科研课题（201940220）

The role of Bmal1 in regulating the aging of mesenchymal stem cells via Wnt/β-catenin signaling pathway

YE Cui, WU Hai-miao

Department of Orthodontics, Shanghai Stomatological Hospital. Shanghai 200031, China

Received:2020-01-07 Revised:2020-04-27 Online:2020-12-25 Published:2021-01-08

摘要/Abstract

摘要： 目的： 探讨Bmal1与Wnt经典信号通路对间充质干细胞（mesenchymal stem cells,MSCs）的衰老是否具有协同或拮抗作用。方法： 分为转染组和空白对照组,转染组用Bmal1的慢病毒转染NIH-3T3细胞表达增强后,通过实时荧光定量PCR和Western免疫印迹等方法检测转染组和空白组β-catenin、TCF1表达量。采用SPSS 19.0软件包对数据进行统计学分析。结果： 经PCR鉴定,Bmal1基因重组慢病毒载体构建成功,转染Bmal1表达增强后,β-catenin表达也显著增强（P<0.05）,但与TCF1增强比例不同;而TCF1的变化无统计学意义。结论： Bmal1对Wnt 经典信号通路直接或间接调控小鼠 BMSCs 的增龄性改变具有协同作用。

关键词: 衰老, 骨髓间充质干细胞, 经典wnt 信号通路, β-catenin, Bmal1

Abstract: PURPOSE: To investigate whether Bmal1 and Wnt/β-catenin signaling pathway has synergistic or antagonistic effects on the aging of mouse bone marrow-derived mesenchymal stem cells (BMSCs). METHODS: The cells were divided into two groups. The expression of β - catenin and TCF1 in the transfected group and the blank group were detected by real-time fluorescent quantitative PCR and Western blot. The data were statistically analyzed using SPSS 19.0 software package. RESULTS: Recombinant Bmal1 lentiviral vector was successfully constructed. The expression of β-catenin was enhanced after Bmal1 was transfected(P<0.05), which was different from that of TCF1. There was no significant difference in TCF1. CONCLUSSIONS: Bmal1 has a synergistic effect on the aging changes of MSCs in mice directly or indirectly regulated by Wnt/β-catenin signaling pathway.

Key words: Aging, Marrow mesenchymal stem cells, Classical Wnt signaling pathway, β-catenin, Bmal1

中图分类号:

R783.5

叶翠, 吴海苗. Bmal1在经典Wnt通路调控间充质干细胞衰老中的作用[J]. 上海口腔医学, 2020, 29(6): 601-605.

YE Cui, WU Hai-miao. The role of Bmal1 in regulating the aging of mesenchymal stem cells via Wnt/β-catenin signaling pathway[J]. Shanghai Journal of Stomatology, 2020, 29(6): 601-605.

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Bmal1在经典Wnt通路调控间充质干细胞衰老中的作用

The role of Bmal1 in regulating the aging of mesenchymal stem cells via Wnt/β-catenin signaling pathway

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