上海口腔医学 ›› 2019, Vol. 28 ›› Issue (3): 225-230.doi: 10.19439/j.sjos.2019.03.001

• 论著 • 上一篇    下一篇

ANXA1对口腔鳞癌TPF化疗敏感性的影响及作用机制探讨

朱东旺*, 孙文文*, 赵铜超, 钟来平, 张志愿   

  1. 上海交通大学医学院附属第九人民医院·口腔医学院 口腔颌面-头颈肿瘤科,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011
  • 收稿日期:2019-02-18 修回日期:2019-04-24 出版日期:2019-06-25 发布日期:2019-08-09
  • 通讯作者: 钟来平,E-mail:zhonglaiping@163.com
  • 作者简介:朱东旺(1986-),男,博士,住院医师,E-mail:zhudongwang@hotmail.com;孙文文(1990-),女,硕士,住院医师,E-mail:sunwenwen9th@126.com。*并列第一作者
  • 基金资助:
    国家自然科学基金(81602370)

The effect and mechanism of ANXA1 on TPF chemosensitivity in oral squamous cell carcinoma

ZHU Dong-wang, SUN Wen-wen, ZHAO Tong-chao, ZHONG Lai-ping, ZHANG Zhi-yuan   

  1. Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011, China;
  • Received:2019-02-18 Revised:2019-04-24 Online:2019-06-25 Published:2019-08-09

摘要: 目的 探讨ANXA1在口腔鳞癌TPF化疗中的作用机制。方法 构建ANXA1过表达及低表达细胞系,通过细胞增殖、细胞毒性实验、实时荧光定量PCR和Western免疫印迹方法,分析ANXA1在TPF化疗中的作用,并探讨ANXA1通过上皮-间质转化(EMT)通路在TPF化疗中的作用机制。采用SPSS18.0软件包对数据进行统计学分析。结果 过表达口腔鳞癌细胞系ANXA1后,细胞生长速率降低,细胞周期减慢,对TPF诱导化药物敏感性降低,口腔鳞癌细胞出现EMT;低表达口腔鳞癌细胞系ANXA1后,细胞生长速率增快,细胞周期加快,对TPF化疗药物敏感性增加,口腔鳞癌细胞出现逆EMT。结论 在口腔鳞癌TPF化疗中,ANXA1过表达导致细胞出现EMT,对化疗敏感性降低。

关键词: 口腔鳞癌, 膜联蛋白A1, 上皮-间质转化, 化疗敏感性

Abstract: PURPOSE: To investigate the mechanism of ANXA1 in TPF chemotherapy of oral squamous cell carcinoma (OSCC). Methods: ANXA1 overexpression and low-expression cell lines were constructed. The role of ANXA1 in TPF chemotherapy was analyzed by cell proliferation, cytotoxicity test, real-time PCR and Western blot, and the mechanism of ANXA1 in TPF chemotherapy through EMT (epithelial-mesenchymal transition) pathway was discussed. The data were analyzed with SPSS 18.0 software package. Results: After overexpression of ANXA1, cell growth rate decreased, cell cycle slowed down, sensitivity to TPF-induced drugs decreased, and EMT occurred in OSCC. After underexpression of ANXA1, cell growth rate increased, cell cycle accelerated, sensitivity to TPF chemotherapeutic drugs increased, and reverse EMT occurred in OSCC. Conclusions: In TPF chemotherapy of OSCC, overexpression of ANXA1 results in EMT of cells, which leads to decreased chemosensitivity.

Key words: Oral squamous cell carcinoma, ANXA1, EMT, Chemosensitivity

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