低能量激光配合牙周基础治疗对糖尿病合并慢性牙周炎患者龈沟液细胞因子及LPS、leptin的影响

doi:10.19439/j.sjos.2018.06.015

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (6): 637-640.doi: 10.19439/j.sjos.2018.06.015

低能量激光配合牙周基础治疗对糖尿病合并慢性牙周炎患者龈沟液细胞因子及LPS、leptin的影响

李凤, 徐华顺

上海市第八人民医院口腔科,上海 200235

收稿日期:2018-03-08 出版日期:2018-12-25 发布日期:2019-01-11
通讯作者: 李凤,E-mail: lif1029@163.com
作者简介:李凤(1979-),女,主治医师

Effects of low level laser combined with basic periodontal therapy on cytokines and LPS, leptin in gingival crevicular fluid of diabetes mellitus complicated with chronic periodontitis patients

LI Feng, XU Hua-shun

Department of Stomatology, Shanghai No.8 People's Hospital. Shanghai 200235, China

Received:2018-03-08 Online:2018-12-25 Published:2019-01-11
Supported by:
上海市卫计委青年项目(20164Y0123）

摘要/Abstract

摘要： 目的: 分析低能量激光照射疗法（low level laser treatment, LLLT）配合牙周基础治疗对糖尿病合并慢性牙周炎（diabetes mellitus complicated with chronic periodontitis, DMCP）患者龈沟内细胞因子及LPS、Leptin的影响。方法: 将2016年1月—2017年1月间收治的80例DMCP患者依照随机信封法分为实验组及对照组,每组40例。对照组采用牙周基础治疗,实验组采用低剂量激光配合牙周基础治疗。治疗前及治疗后3个月检测患者口腔PD、SBI及CAL水平;采集患者空腹静脉血,检测血液中糖代谢指标;采集患者龈沟液,检测龈沟液中炎症因子水平。采用SPSS19.0软件包对数据进行统计学分析。结果: 经治疗后2组CAL、PD及SBI水平均显著降低（P<0.05）,治疗后实验组CAL、PD及SBI水平显著低于对照组（P<0.05）。治疗后2组患者血中FBG及HbA1c水平均显著降低（P<0.05）,治疗后实验组FBG及HbA1c水平显著低于对照组（P<0.05）。治疗后2组患者龈沟液中hs-CRP、IL-1β及TNF-α水平均显著降低（P<0.05）,治疗后实验组患者龈沟液中hs-CRP、IL-1β及TNF-α水平显著低于对照组（P<0.05）。治疗后2组患者龈沟液LPS显著降低,Leptin水平显著升高（P<0.05）,实验组龈沟液中LPS显著低于对照组,Leptin水平显著高于对照组（P<0.05）。结论: 低能量激光配合牙周基础治疗,可有效降低DMCP患者血糖水平及龈沟内LPS水平,提高Leptin水平,显著改善病灶区炎症反应,提高临床疗效。

关键词: 低能量激光, 牙周基础治疗, 糖尿病合并慢性牙周炎, 脂多糖, 瘦素

Abstract: PURPOSES: To analyze the effect of low level laser treatment combined with basic therapy periodontal on cytokines and lipopolysaccharide, leptin in gingival sulcus of patients with diabetes mellitus complicated with chronic periodontitis (DMCP). METHODS: From January 2016 to January 2017, 80 patients with DMCP were randomly divided into experimental group and control group. Patients in the control group received basic periodontal therapy, while patients in the experimental group received low dose laser combined with basic periodontal treatment. Before treatment and 3 months after treatment, oral PD, SBI and CAL levels were detected in all patients. Venous blood was collected, HbA1c and FPG levels in blood serum were detected. TNF-α, IL-1, hs-CRP, LPS, leptin in gingival crevicular fluid were detected. Statistical analyses were performed using SPSS19.0 software package. RESULTS: After treatment, CAL, PD, SBI, FBG, HbA1c, TNF-α, IL-1, hs-CRP, LPS levels were significantly decreased, Leptin level increased in both group, and the difference was significant (P<0.05). After treatment, CAL, PD, SBI, FBG, HbA1c, TNF-α, hs-CRP, IL-1 and the level of LPS in the experimental group was significantly lower than the control group (P<0.05), and the level of leptin was significantly higher than that of the control group (P<0.05). CONCLUSIONS: Low level laser combined with basic periodontal treatment can effectively reduce LPS level of DMCP patients in gingival sulcus, and improve the level of Leptin, significantly improve the inflammatory reaction and clinical efficacy.

Key words: Low level laser, Basic periodontal treatment, Diabetes mellitus complicated with chronic periodontitis, Lipopolysaccharide, Leptin

中图分类号:

R780.1

李凤, 徐华顺. 低能量激光配合牙周基础治疗对糖尿病合并慢性牙周炎患者龈沟液细胞因子及LPS、leptin的影响[J]. 上海口腔医学, 2018, 27(6): 637-640.

LI Feng, XU Hua-shun. Effects of low level laser combined with basic periodontal therapy on cytokines and LPS, leptin in gingival crevicular fluid of diabetes mellitus complicated with chronic periodontitis patients[J]. Shanghai Journal of Stomatology, 2018, 27(6): 637-640.

参考文献

[1] Pradeep AR, Garg V, Raju A, et al.Adjunctive local delivery of aloe vera gel in patients with type 2 diabetes and chronic periodontitis: a randomized, controlled clinical trial[J]. J Periodontol, 2016, 87(3): 268-274.
[2] 陈汀兰, 宋忠臣, 束蓉. 慢性牙周炎与2型糖尿病的关系研究进展[J]. 口腔医学, 2015, 35(4): 306-309.
[3] Turner RT, Dube M, Branscum AJ, et al.Hypothalamic leptin gene therapy reduces body weight without accelerating age-related bone loss[J]. J Endocrinol, 2015, 227(3): 129-141.
[4] Oude Nijhuis CSM, Vellenga E, Daenen SMGJ, et al.Lipopolysaccharide-binding protein: a possible diagnostic marker for Gram-negative bacteremia in neutropenic cancer patients[J]. Intensive Care Med, 2003, 29(12): 2157-2161.
[5] Ge MK, He WL, Chen J, et al.Efficacy of low-level laser therapy for accelerating tooth movement during orthodontic treatment: a systematic review and meta-analysis[J]. Lasers Med Sci, 2015, 30(5): 1609-1618.
[6] Doeuk C, Hersant B, Bosc R, et al.Current indications for low level laser treatment in maxillofacial surgery: a review[J]. Br J Oral Maxillofac Surg, 2015, 53(4): 309-315.
[7] Amadori F, Bardellini E, Conti G, et al.Low-level laser therapy for treatment of chemotherapy-induced oral mucositis in childhood: a randomized double-blind controlled study[J]. Lasers Med Sci, 2016, 31(6): 1231-1236.
[8] Santos MT, Diniz MB, Gouw-Soares SC, et al.Evaluation of low-level laser therapy in the treatment of masticatory muscles spasticity in children with cerebral palsy[J]. J Biomed Opt, 2016, 21(2): 28001-28011.
[9] Goncalves ED, Souza PS, Lieberknecht V, et al.Low-level laser therapy ameliorates disease progression in a mouse model of multiple sclerosis[J]. Autoimmunity, 2016, 49(2): 132-143.
[10] 李萌萌, 赵燕娟, 祝书金, 等. 低能量激光对2型糖尿病合并慢性牙周炎患者血糖代谢指标及超敏C反应蛋白的影响[J]. 国际生物医学工程杂志, 2016, 39(1): 24-27.
[11] 刘锋, 张颖奇. 牙周炎基础治疗对龈沟液中炎症因子水平影响的研究[J]. 中国实验诊断学, 2015, 19(5): 828-830.
[12] 潘先文, 陶学育. 米诺环素软膏治疗慢性牙周炎的疗效及对患者龈沟液中超敏C反应蛋白和白介素10水平影响[J]. 中国药师, 2015, 18(1): 89-91.
[13] Gamsiz-Isik H, Kiyan E, Bingol Z, et al.Does obstructive sleep apnea increase the risk for periodontal disease? a case-control study[J]. J Periodontol, 2017, 88(5):443-448.
[14] 许丽华, 许尧生. 牙周致病菌主要毒力因子脂多糖受体TLR4与牙周炎关系的研究进展[J]. 现代口腔医学杂志, 2015, 29(5): 304-307.
[15] 于倩, 曾克武, 王学美. 酪氨酸蛋白激酶信号转导子与转录激活子信号通路在干预神经系统炎症反应中的作用及意义[J]. 中国药学杂志, 2015, 50(19): 1655-1659.

低能量激光配合牙周基础治疗对糖尿病合并慢性牙周炎患者龈沟液细胞因子及LPS、leptin的影响

Effects of low level laser combined with basic periodontal therapy on cytokines and LPS, leptin in gingival crevicular fluid of diabetes mellitus complicated with chronic periodontitis patients

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	董家辰, 束蓉. 炎症微环境对人牙周膜成纤维细胞增殖与成骨分化的影响[J]. 上海口腔医学, 2022, 31(3): 243-247.
[2]	王菲菲, 饶定成, 金思宇, 杨沛鸣, 周永梅, 万光勇. 蛇床子素对脂多糖诱导的巨噬细胞极化和炎症反应的影响[J]. 上海口腔医学, 2022, 31(3): 255-259.
[3]	王瑞, 杨谛, 于雅琼, 郭佳杰, 仇丽鸿. 牙髓卟啉单胞菌内毒素对成骨细胞分化的抑制作用[J]. 上海口腔医学, 2021, 30(4): 350-354.
[4]	徐方芳, 蒋备战. DKK1对脂多糖感染人牙髓细胞生物学行为的影响[J]. 上海口腔医学, 2021, 30(4): 355-359.
[5]	于雅琼, 尉鹏功, 李晓琳, 曲柳, 孙海燕, 仇丽鸿. 白藜芦醇通过上调SOCS-3蛋白抑制脂多糖诱导的成骨细胞表达MIP-2[J]. 上海口腔医学, 2021, 30(3): 232-236.
[6]	赵斌, 张云鹏, 徐欣. 炎症微环境下芦丁对牙周膜干细胞成骨分化能力的影响[J]. 上海口腔医学, 2019, 28(4): 356-361.
[7]	于雅琼, 李晓琳, 仇丽鸿, 曲柳, 杨谛, 王慧君. 白藜芦醇对牙髓卟啉单胞菌脂多糖诱导成骨细胞产生MIP-2的影响[J]. 上海口腔医学, 2019, 28(2): 128-132.
[8]	钟兆琪, 付华. SPR牙周基础治疗对慢性牙周炎患者龈沟液C反应蛋白的影响[J]. 上海口腔医学, 2018, 27(5): 530-534.
[9]	李晓琳, 于雅琼, 仇丽鸿, 郭佳杰, 邵丽娜, 王丝墨, 杨谛. 牙髓卟啉单胞菌脂多糖对小鼠成骨细胞表达MCP-1的影响[J]. 上海口腔医学, 2018, 27(1): 1-5.
[10]	于雅琼, 李晓琳, 仇丽鸿, 郭佳杰, 杨谛, 郭艳. 牙髓卟啉单胞菌脂多糖对小鼠成骨细胞表达MIP-1α的影响[J]. 上海口腔医学, 2017, 26(3): 263-267.
[11]	韩亚琨, 林晓萍. 相同基因条件下牙周治疗与动脉硬化的相关性[J]. 上海口腔医学, 2017, 26(2): 209-212.
[12]	于雅琼, 郭佳杰, 仇丽鸿, 李晓琳, 杨谛, 郭艳. 牙髓卟啉单胞菌脂多糖对小鼠成骨细胞表达IL-34 mRNA的影响[J]. 上海口腔医学, 2017, 26(1): 37-41.
[13]	郑宝玉, 何梦娇, 江俊, 骆凯, 陈玉玲, 闫福华. 瘦素基因修饰组织工程化复合物的体外构建[J]. 上海口腔医学, 2016, 25(6): 641-646.
[14]	高磊, 孙晓菊, 谢洪, 南顺花, 谢慧心. 牙周基础治疗对脑卒中高危人群伴中重度牙周炎患者血清 sCD40L及牙周临床指标的影响[J]. 上海口腔医学, 2016, 25(5): 574-578.
[15]	李欣, 洪弘, 张延清, 韦曦. 大鼠牙乳头细胞旁分泌效应对巨噬细胞分泌炎症因子的调控作用[J]. 上海口腔医学, 2016, 25(4): 396-402.