慢性牙周炎患者唾液中miR-146a的表达及其与龈沟炎症、MMP-8/TIMP-1水平的关系

doi:10.19439/j.sjos.2018.03.020

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (3): 309-312.doi: 10.19439/j.sjos.2018.03.020

慢性牙周炎患者唾液中miR-146a的表达及其与龈沟炎症、MMP-8/TIMP-1水平的关系

高颖，郝晨笛

郑州大学附属郑州中心医院口腔颌面外科，河南郑州 450007

收稿日期:2017-11-13 修回日期:2018-01-30 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: 高颖，E-mail：987313874@qq.com
作者简介:高颖（1983-），女，硕士研究生，主治医师

Expression of miR-146a in saliva of chronic periodontitis patients and its influence on gingival crevicular inflammation and MMP-8/TIMP-1 levels

GAO Ying, HAO Chen-di.

Department of Oral and Maxillofacial Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University. Zhengzhou 450007, Henan Province, China

Received:2017-11-13 Revised:2018-01-30 Online:2018-07-20 Published:2018-07-20

摘要/Abstract

摘要： 目的： 探讨慢性牙周炎患者唾液中miR-146a的表达及其与龈沟炎症、基质金属蛋白酶8（MMP-8）、基质金属蛋白酶抑制剂1（TIMP-1）水平的关系。方法： 选择2015年3月—2017年1月间收治的慢性牙周炎患者68例作为慢性牙周炎组，同期在本院进行体检的健康志愿者50例作为正常对照组。检测2组研究对象唾液中miR-146a的表达量，龈沟液中炎症因子、MMP-8/TIMP-1的水平及牙周临床症状指标。采用SPSS24.软件中的Pearson检验评估慢性牙周炎患者唾液中miR-146a的表达量与病情严重程度的相关关系。结果： 慢性牙周炎患者唾液中miR-146a的表达量显著高于正常对照组（P<0.05），龈沟液中炎症因子（IL-1β、IL-6、IL-35、TNF-α）水平、牙周临床症状指标（PD、AL、PLI、BI）以及龈沟液中MMP-8、TIMP-1的水平显著高于正常对照组（P<0.05）。Pearson检验发现，慢性牙周炎患者唾液中miR-146a表达量与龈沟炎症程度、牙周临床症状严重程度及MMP-8/TIMP-1水平呈正相关。结论： 慢性牙周炎患者唾液中miR-146a表达量异常增高，且与龈沟炎症程度、牙周损伤程度一致。

关键词: 慢性牙周炎, miR-146a, 龈沟炎症, MMP-8/TIMP-1

Abstract: PURPOSE: To study the expression of miR-146a in saliva of chronic periodontitis patients and its influence on gingival crevicular inflammation and MMP-8/TIMP-1 levels. METHODS: Sixty-eight patients with chronic periodontitis treated in our hospital during March 2015 to January 2017 were chosen as the experimental group, while 50 healthy volunteers for physical examination were chosen as the control group. The level of miR-146a in saliva and inflammatory factors, MMP-8/TIMP-1 in gingival crevicular fluid were detected. Pearson test was used to assess the correlation between expression of miR-146a in saliva and the severity of disease with SPSS24.0 software package. RESULTS: The expression of miR-146a in saliva in the experimental group was significantly higher than that in the control group(P<0.05). The level of inflammatory cytokines (IL-1β, IL-6 and IL- 35, TNF-α), the indexes of periodontal clinical symptoms (PD, AL, PLI, BI) in the experimental group were significantly higher than those in the control group (P <0.05); and MMP-8 and TIMP-1 in gingival crevicular fluid in the experimental group were significantly higher than those in the control group(P<0.05). Pearson test found that the expression of miR-146a in saliva of patients with chronic periodontitis was positively correlated with the degree of gingival inflammation, the severity of periodontal symptoms and the level of MMP-8/TIMP-1. CONCLUSIONS: The expression of miR-146a in saliva of chronic periodontitis patients is abnormally high, and the specific expression is consistent with the degree of gingival inflammation and periodontal injury.

Key words: Chronic periodontitis, miR-146a, Gingival sulcus inflammation, MMP-8/TIMP-1

中图分类号:

R781.4.2

高颖，郝晨笛. 慢性牙周炎患者唾液中miR-146a的表达及其与龈沟炎症、MMP-8/TIMP-1水平的关系[J]. 上海口腔医学, 2018, 27(3): 309-312.

GAO Ying, HAO Chen-di.. Expression of miR-146a in saliva of chronic periodontitis patients and its influence on gingival crevicular inflammation and MMP-8/TIMP-1 levels[J]. Shanghai Journal of Stomatology, 2018, 27(3): 309-312.

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慢性牙周炎患者唾液中miR-146a的表达及其与龈沟炎症、MMP-8/TIMP-1水平的关系

Expression of miR-146a in saliva of chronic periodontitis patients and its influence on gingival crevicular inflammation and MMP-8/TIMP-1 levels

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