不同浓度木通皂苷D对大鼠正畸牙移动的影响

doi:10.19439/j.sjos.2018.02.004

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (2): 129-134.doi: 10.19439/j.sjos.2018.02.004

不同浓度木通皂苷D对大鼠正畸牙移动的影响

崔金婕¹, 王旭霞^2,3, 王媛⁴, 陈佩佩¹, 马丹¹, 张君^1,3

1.山东大学口腔医学院正畸科, 2.口腔颌面外科教研室,山东济南 250012;
3.山东省口腔组织再生重点实验室,山东济南 250012;
4.浙江大学医学院附属口腔医院,浙江杭州 310006

收稿日期:2017-09-14 修回日期:2017-11-20 出版日期:2018-04-25 发布日期:2018-05-14
通讯作者: 张君,E-mail: zhangj@sdu.edu.cn
作者简介:崔金婕（1992-）,女,硕士, E-mail：cjj_cc@126.com
基金资助:
国家自然科学基金（81371180）; 山东省科技发展计划（ 2014GGH218024）

Effect of akebiasaponin D with different concentrations on orthodontic tooth movement in rats

CUI Jin-jie¹, WANG Xu-xia^2,3, WANG Yuan⁴, CHEN Pei-pei¹, MA Dan¹, ZHANG Jun^1,3

1.Department of Orthodontics, 2.Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University. Jinan 250012, Shandong Province;
3.Key Laboratory of Oral Tissue Regeneration of Shandong Province. Jinan 250012, Shandong Province;
4.Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University. Hangzhou 310006, Zhejiang Province, China

Received:2017-09-14 Revised:2017-11-20 Online:2018-04-25 Published:2018-05-14

摘要/Abstract

摘要： 目的:探讨局部注射不同浓度的木通皂苷D对大鼠正畸牙移动的影响。方法:40只6周龄雌性Wistar大鼠,随机分为4组,建立正畸牙移动动物模型。以上颌两中切牙为支抗,牵引上颌第一磨牙向近中移动,镍钛拉簧加力40 g。ASD1组以5 mg/kg的剂量局部注射ASD溶液;ASD2组以10 mg/kg的剂量局部注射ASD溶液;PGE2组以25 μg/kg的剂量局部注射PGE2溶液;空白对照组注射生理盐水。4组动物分别于正畸加力3、7、14、21、28 d后分批处死,测量各期上颌第一磨牙的移动距离,H-E染色观察切片牙周组织及破骨细胞数量的变化,采用SPSS13.0软件包对数据进行统计学分析。结果:各实验组动物牙移动距离均大于对照组,加力第3天,只有PGE2组与对照组相比有显著差异（P<0.05）。加力第7天,ASD2组和PGE2组与对照组相比均有显著差异（P<0.05）。加力第14、21、28天,ASD1组、ASD2组和PGE2组与对照组相比均具有统计学意义（P<0.05）。各时间段ASD2组和PGE2组之间牙移动距离相比无统计学意义（P>0.05）。组织学观察,压力侧多核破骨细胞数目随时间推移呈上升趋势,第21天达到高峰,随后逐渐减少。结论:局部注射ASD可促进大鼠正畸牙移动,ASD以10 mg/kg的剂量局部注射,其作用与PGE2促进正畸牙移动的效果相似,较5 mg/kg剂量的ASD促进牙移动的效果明显。

关键词: 木通皂苷D, 正畸牙移动, 破骨细胞

Abstract: PURPOSE: This study investigated the effect of local injection of akebiasaponin D (ASD) with different concentrations on the rate of orthodontic tooth movement in rats. METHODS: Forty 6-week-old female Wistar rats were randomly divided into 4 groups with 10 rats in each group. Animal model for orthodontic tooth movement was established. The upper first molars of the rats were moved mesially by coil springs（force=40 g）. ASD solution at the concentration of 5 mg/kg was locally injected in ASD1 group, and ASD solution at the concentration of 10 mg/kg was locally injected in ASD2 group. The rats of group PGE2 were injected PGE2 solution at the concentration of 25 μg/kg. The same amount of normal saline was injected in the control group. The rats were sacrificed in batch on the 3rd, 7th, 14th, 21st and 28th days after orthodontic treatment. The distance between upper first molar and second molar was measured. H-E straining was performed to observe the changes of periodontal tissue and the amount of osteoclast. SPSS13.0 software package was used for statistical analysis. RESULTS: The distance between the first and second molar was successively increased compared with the control group. On day 3, there was significant difference between PGE2 group and the control group (P<0.05). On day 7, the distance between the first and second molar in PGE2 group and ASD2 group was significantly increased compared with the control group (P<0.05). The amount of tooth movement was significantly increased (P<0.05) in ASD1 group, ASD2 group and PGE2 group, compared with the control group on the 14th, 21st and 28th day. However, there was no significant difference (P>0.05) between ASD2 group and PGE2 group. Under microscope, the number of osteoclast was increased on the tension side, reaching a peak on day 21st, and decreased later. CONCLUSIONS: Local injection of ASD solution may accelerate orthodontic tooth movement. ASD solution at the dose of 10 mg/kg can accelerate orthodontic tooth movement efficiently similar to PGE2 solution, while ASD solution at the dose of 5 mg/kg is not as effectual as PGE2 solution.

Key words: Akebiasaponin D, Orthodontic tooth movement, Osteoclast

中图分类号:

R783.5

崔金婕, 王旭霞, 王媛, 陈佩佩, 马丹, 张君. 不同浓度木通皂苷D对大鼠正畸牙移动的影响[J]. 上海口腔医学, 2018, 27(2): 129-134.

CUI Jin-jie, WANG Xu-xia, WANG Yuan, CHEN Pei-pei, MA Dan, ZHANG Jun. Effect of akebiasaponin D with different concentrations on orthodontic tooth movement in rats[J]. Shanghai Journal of Stomatology, 2018, 27(2): 129-134.

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不同浓度木通皂苷D对大鼠正畸牙移动的影响

Effect of akebiasaponin D with different concentrations on orthodontic tooth movement in rats

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