上海口腔医学 ›› 2016, Vol. 25 ›› Issue (4): 403-408.

• 论 著 • 上一篇    下一篇

不同放疗剂量对人唾液腺腺样囊性癌细胞生物学行为的影响

黄育萌1, 2, 王颖3, 李勇1, 刘平1, 柳先锋3, 赵洪伟1, 4   

  1. 1.重庆医科大学附属口腔医院 口腔颌面外科,重庆 401147; 2.口腔疾病与生物医学重庆市重点实验室,重庆 401147; 3.重庆市肿瘤医院 肿瘤放射治疗科,重庆 400030; 4.重庆市高校市级口腔生物医学工程重点实验室,重庆 401147
  • 收稿日期:2016-01-29 出版日期:2016-08-25 发布日期:2016-09-06
  • 通讯作者: 赵洪伟,E-mail:bradfordwei@163.com
  • 作者简介:黄育萌(1988-),男,硕士研究生,E-mail:huangymcq@163.com
  • 基金资助:
    重庆市医学科研计划(2015ZDXM019)

Effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells

HUANG Yu-meng1, 2, WANG Ying3, LI Yong1, LIU Ping1, LIU Xian-feng3, ZHAO Hong-wei1, 4   

  1. 1.Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University. Chongqing 401147;
    2.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences. Chongqing 401147;
    3.Department of Tumor Radiotherapy of Chongqing Tumor Hospital. Chongqing 400030;
    4. Colleges and Universities in Chongqing Municipal Oral Key Laboratory of Biomedical Engineering. Chongqing 401147, China
  • Received:2016-01-29 Online:2016-08-25 Published:2016-09-06

摘要: 目的探讨不同放疗剂量对人唾液腺腺样囊性癌(SACC-83、SACC-LM)细胞生物学行为的影响。方法采用不同放疗剂量(0、2、4、6、8、10 Gy)作用SACC-83、SACC-LM细胞并继续培养48 h,CCK-8检测细胞存活,流式细胞仪检测细胞凋亡和细胞周期,细胞划痕实验观察细胞迁移能力变化。采用SPSS19.0软件包对数据进行统计学分析。结果放疗对SACC-LM细胞的细胞存活率、细胞凋亡率、异倍体、细胞迁移力的影响较SACC-83显著(P<0.05);与其他放疗剂量相比,SACC-83细胞在6 Gy剂量照射下细胞存活率最低,细胞凋亡比例及异倍体比例最高,细胞迁移能力最弱。结论SACC-LM细胞放疗敏感性较SACC-83更高,SACC-83细胞对6 Gy剂量放疗最为敏感。

关键词: 唾液腺, 腺样囊性癌, 放疗敏感性, 细胞凋亡

Abstract: PURPOSE: To explore the effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells (SACC-83, SACC-LM). METHODS: Different doses of radiation (0, 2, 4, 6, 8, 10 Gy) were applied to SACC-83, SACC-LM cells and the cells were continued to culture for 48 h. CCK-8 test, flow cytometry(FCM) and cell scratch experiment were used to observe cell proliferation, apoptosis and cell migration. SPSS19.0 software package was used for data analysis. RESULTS: The effect of radiation on SACC-LM cells survival rate, cell apoptosis, heteroploid and cell migration ability were significantly greater than that on SACC-83 cells (P<0.05). In SACC-83 cells, compared with other doses of radiation, 6 Gy of irradiation was the most sensitive on cell survival rate, cell apoptosis, heteroploid and cell migration ability. CONCLUSIONS: Radiation sensitivity of SACC-LM was greater than that of SACC-83 cells. With 6 Gy of radiation, changes of biology in SACC-83 cells most significant than other doses of radiation.

Key words: Salivary gland, Adenoid cystic carcinoma, Radiation sensitivity, Cell apoptosis.

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