新型牙髓治疗材料iRoot BP Plus对人牙龈成纤维细胞的细胞毒性

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (6): 681-684.

新型牙髓治疗材料iRoot BP Plus对人牙龈成纤维细胞的细胞毒性

史爽¹, 包志凡^{1, 2}, 陈旭^{1, 2}, 张丹丹¹

1.中国医科大学口腔医学院儿童口腔科,2.辽宁省口腔医学研究所儿童口腔医学研究室,辽宁沈阳 110002

收稿日期:2014-03-21 修回日期:2014-05-12 出版日期:2014-12-20 发布日期:2015-01-08
通讯作者: 陈旭,Tel：024-22895782,E-mail：chenxu_cmu@sina.com E-mail:shuangshi@hotmail.com
作者简介:史爽（1986-）,女,在读硕士研究生
基金资助:
辽宁省科学技术计划项目（2012225015）; 沈阳市科学技术计划项目（F12-277-1-65）

Cytotoxicity of a novel endodontic treatment material iRoot BP Plus to human gingival fibroblasts

SHI Shuang, BAO Zhi-fan, CHEN Xu, ZHANG Dan-dan

1.Department of Pediatric Dentistry, School of Stomatology, China Medical University,
2. Laboratory of Pediatric Dentistry, Liaoning Research Institute of Stomatology. Shenyang 110002, Liaoning Province, China.

Received:2014-03-21 Revised:2014-05-12 Online:2014-12-20 Published:2015-01-08
Supported by:
Science and Technology Project of Liaoning Province (2012225015) and Shenyang City (F12-277-1-65)

摘要/Abstract

摘要： 目的：比较新型牙髓治疗材料iRoot BP Plus与矿物三氧化物凝聚体（mineral trioxide aggregate, MTA)对人牙龈成纤维细胞的细胞毒性。方法：采用四甲基偶氮唑盐（methyl-thiazol-tetrazolium, MTT)法测定上述2种材料不同浸提时间（1、3、7 d）的浸提液原液及不同浓度稀(1:2、1:5)释液对体外培养的人牙龈成纤维细胞增殖的影响。细胞增殖率以表示,采用SPSS20.0软件包对数据进行析因设计的方差分析。结果：iRoot BP Plus和MTA的浸提液原液及不同浓度稀释液作用后的细胞增殖率界于77.31%~113.82%,细胞毒性分级为0或1级,评价结果为无细胞毒性。不同时间点、不同稀释度下,2种材料对体外培养的人牙龈成纤维细胞增殖率的影响无显著差异（F_{浓度*时间*材料}=1.393,P=0.256）。结论：iRoot BP Plus与MTA均对体外培养的人牙龈成纤维细胞无细胞毒性。

关键词: iRoot BP Plus, 矿物三氧化物凝聚体, 细胞毒性, 人牙龈成纤维细胞

Abstract: PURPOSE: To compare the cytotoxicity of a novel endodontic treatment material iRoot BP Plus and mineral trioxide aggregate (MTA) to human gingival fibroblasts in vitro. METHODS: Cultured human gingival fibroblasts were exposed to multiple concentrations of material elutes (no dilution, 1:2 dilution, and 1:5 dilution). The test material samples were immersed and incubated in the culture medium for 1, 3 or 7 days at 37℃. The proliferation rate was evaluated using methylthiazol tetrazolium (MTT) assay. Cell relative growth rates were presented as . The data was statistically analyzed by factorial design ANOVA using SPSS 20.0 software package. RESULTS: Cell relative growth rates of the eluates of iRoot BP Plus and MTA in different concentrations ranged from 77.31% to 113.82%. The cytotoxicity grade of both materials was 0 or 1 (no cytotoxicity). There was no significant difference in the relative growth rate in different concentrations of iRoot BP plus and MTA eluates under different elution times (F_{concentration*time*material}=1.393, P=0.256). CONCLUSIONS: Both iRoot BP Plus and MTA exhibit minimal level of cytotoxicity.

Key words: iRoot BP Plus, Mineral trioxide aggregate, Cytotoxicity, Human gingival fibroblasts

中图分类号:

R781.05

史爽, 包志凡, 陈旭, 张丹丹. 新型牙髓治疗材料iRoot BP Plus对人牙龈成纤维细胞的细胞毒性[J]. 上海口腔医学, 2014, 23(6): 681-684.

SHI Shuang¹, BAO Zhi-fan^{1, 2}, CHEN Xu^{1, 2}, ZHANG Dan-dan¹. Cytotoxicity of a novel endodontic treatment material iRoot BP Plus to human gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2014, 23(6): 681-684.

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