上海口腔医学

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (5): 561-565.

• 基础研究 • 上一篇    下一篇

纯中药口腔含漱液对皮肤黏膜的刺激性及杀菌作用

马牧之1, 刘昭君1, 陆佳彤2, 陈功培1, 余飞1, 郑园娜1   

  1. 1.浙江中医药大学 口腔医学院; 2. 浙江中医药大学 药学院,浙江 杭州 310053
  • 收稿日期:2014-03-17 修回日期:2014-06-01 出版日期:2014-10-20 发布日期:2015-02-04
  • 通讯作者: 郑园娜,E-mail:zyn218@126.com
  • 作者简介:马牧之(1991-),男,在读本科生,E-mail:mamuzhi@gmail.com
  • 基金资助:
    浙江省大学生科技创新项目（2012R410028）

Experimental evaluation of pure traditional Chinese medical mouth rinse on skin and mucosa stimulation and bacteriostatic-bactericidal effect

MA Mu-zhi1, LIU Zhao-jun1, LU Jia-tong2, CHEN Gong-pei1, YU Fei1, ZHENG Yuan-na1   

  1. 1.School of Stomatology; 2.School of Medicine, Zhejiang Chinese Medical University. Hangzhou 310053, Zhejiang Province, China
  • Received:2014-03-17 Revised:2014-06-01 Online:2014-10-20 Published:2015-02-04
  • Supported by:
    ; Supported by Science and Technology Projects for College Students in Zhejiang Province (2012R410028)

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摘要： 目的 观察纯中药口腔含漱液对皮肤、黏膜的刺激反应及对口腔常见细菌的杀菌效果。方法 分别用自制纯中药含漱液和生理盐水对14只5个月龄家兔滴左、右眼7 d;并涂抹10只7个月龄豚鼠左、右侧臀部、腹部、背部（涂药前脱毛）,左右对照,分别于涂抹后第3、7天各处死5只,制作病理切片。分别将金黄色葡萄球菌、大肠埃希氏菌、白念菌、铜绿假单胞菌、乙型链球菌制取的菌悬液加入含不同浓度纯中药含漱液或复方硼砂含漱液（朵贝尔 液）的营养液中,观察细菌的生长情况。分别将黏性放线菌、消化链球菌、核梭杆菌制取的菌液加入等2倍稀释的复方硼砂溶液和纯中药含漱液中,观察有无细菌生长。采用 SPSS17.0软件包对数据进行统计学分析。结果 家兔右眼结膜与对照组相比,无明显充血、发红等变化;豚鼠右侧臀部、腹部、背部皮肤与对照组相比,无明显红肿、皮炎等变化。纯中药含漱液对上述需氧菌和厌氧菌的杀菌效果均显著高于对照组。结论 纯中药含漱液对皮肤、黏膜无刺激反应,对口腔常见细菌有杀菌效果。

关键词: 纯中药含漱液, 牙周病, 皮肤黏膜刺激实验, 杀菌实验

Abstract: PURPOSE: To observe the stimulating effect of pure traditional Chinese medical mouth rinse (PTCMMR) on skin and mucosa and to evaluate the efficacy of PTCMMR on inhibiting and killing oral common bacteria. METHODS: PTCMMR and normal saline (NS) were separately dripped onto 14 five-month rabbits' left and right eyes for 7 days, and were smeared on the left and right side of buttocks, abdomens, back of 10 seven-month guinea pigs (after hair removal) once a day for comparison between the two sides. Five animals were sacrificed after 3 and 7 days of smearing to make pathological sections. Bacterium suspension with Staphylococcus aureus, Escherichia coli, Candida albicans, Pseudomonas aeruginosa and Streptococcus B were dropped to nutrient solution containing different concentrations of PTCMMR or compound Borax solution to observe the growth situation of bacteria. Inhibition zone diameters were measured after 18h of incubation within 37℃ incubator. The data was statistically analyzed using SPSS 17.0 software package. RESULTS: There was no obvious change such as redness, dermatitis between rabbits’ left eye conjunctiva and the control group. The bacteriostatic and bactericidal effect of anaerobe and aerobe with dropping of PTCMMR was stronger than that of control group. CONCLUSIONS: PTCMMR has no stimulation to skin and mucosa, while it has strong efficacy to inhibit and kill oral common bacteria.

Key words: Pure traditional Chinese medical mouth rinse, Periodontal disease, Skin and mucosa stimulation test, Bacteriostatic and bactericidal test