间歇性完全鼻阻塞诱导幼年大鼠颏舌肌低氧诱导因子1α的表达

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (2): 138-142.

间歇性完全鼻阻塞诱导幼年大鼠颏舌肌低氧诱导因子1α的表达

刘炯, 陈金东, 聂萍, 盛潇, 赵彦惠, 徐晓珑, 陶丽, 朱敏

上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科, 上海市口腔医学重点实验室, 上海 200011

收稿日期:2013-08-19 出版日期:2014-04-20 发布日期:2014-05-21
通讯作者: 朱敏, E-mail:zminnie@126.com　
作者简介:刘炯(1988-), 男, 硕士研究生, E-mail:liujiong030288@163.com
基金资助:
上海市科学技术委员会资助项目(11140902001);上海市科学技术委员会实验动物专项基金

Expression of HIF-1α in the genioglossus associated with induced bilateral intermittent nasal obstruction in young rats

LIU Jiong, CHEN Jin-dong, NIE Ping, SHENG Xiao, ZHAO Yan-hui, XU Xiao-long, TAO Li, ZHU Min

Department of Oral and Cranio-maxillofacial Science, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;
Shanghai Key Laboratary of Stomatology. Shanghai 200011, China

Received:2013-08-19 Online:2014-04-20 Published:2014-05-21

摘要/Abstract

摘要： 目的: 研究幼鼠在双侧间歇性鼻阻塞时低氧诱导因子1α(hypoxia inducible factor, HIF-1α)的表达变化, 探讨双侧鼻阻塞对生长发育期大鼠颏舌肌的影响。方法: 将30只4周龄SD大鼠(Sprague-Dawley rat, SD rat)分为3组。A组为双侧鼻阻塞组, 常氧条件下鼻孔双侧阻塞4 h(8:00 am-12:00 am), 分别阻塞21 d和55 d;B组为单侧鼻阻塞组, 常氧条件下鼻孔单侧阻塞4 h/d(8:00 am-12:00 am), 分别阻塞21 d和55 d;C组为对照组, 常氧条件下饲养。取出颏舌肌, 分别进行H-E染色和免疫组织化学染色, 检测颏舌肌中HIF-1α的表达, 采用SPSS13.0软件包对数据进行统计学分析。结果: 在双侧鼻阻塞组, 颏舌肌HIF-1α的表达随鼻阻塞时间的延长而增加, 单侧鼻阻塞组以及对照组中颏舌肌均无HIF-1α表达。结论: 在双侧鼻阻塞的未成年大鼠中, 颏舌肌表达HIF-1α, 并且随着鼻阻塞时间的延长, HIF-1α的表达不断增加。

关键词: 鼻阻塞, 低氧诱导因子, 颏舌肌

Abstract: PURPOSE: To investigate the expression of HIF-1α in the genioglossus associated with induced bilateral intermittent nasal obstruction in young rats. METHODS: Thirty 4-week-old SD rats were employed and equally divided into 3 groups. In group A, both nostrils were occluded by nose plugs. In group B, the right nostril was occluded. In group C, no obstruction of the nose was performed as control group. The obstruction time was from 8 am to 12 am everyday, and the period was 21 d and 55 d. The genioglossus was taken for HE, and immunohistochemical staining was used to detect the expression of HIF-1α. The data was analyzed with SPSS 13.0 software package. RESULTS: The rats were sacrificed at the 21th day and 55th day, respectively. The expression of HIF-1α in group A was significantly higher than that in group B and group C, and became stronger with the increasing of obstruction time. CONCLUSIONS: Oral breathing caused by bilateral intermittent nasal obstruction in young rats results in overexpression of HIF-1α in the genioglossus.

Key words: Nasal obstruction, Genioglossus, HIF-1α

中图分类号:

R782.2

刘炯, 陈金东, 聂萍, 盛潇, 赵彦惠, 徐晓珑, 陶丽, 朱敏. 间歇性完全鼻阻塞诱导幼年大鼠颏舌肌低氧诱导因子1α的表达[J]. 上海口腔医学, 2014, 23(2): 138-142.

LIU Jiong, CHEN Jin-dong, NIE Ping, SHENG Xiao, ZHAO Yan-hui, XU Xiao-long, TAO Li, ZHU Min. Expression of HIF-1α in the genioglossus associated with induced bilateral intermittent nasal obstruction in young rats[J]. Shanghai Journal of Stomatology, 2014, 23(2): 138-142.

参考文献

[1] Posnick JC, Agnihotri N. Consequences and management of nasal airway obstruction in the dentofacial deformity patient [J]. Curr Opin Otolaryngol Head Neck Surg, 2010, 18(4): 323-331.
[2] Defabjanis P. Impact of nasal airway obstruction on dentofacial development and sleep disturbances in children: preliminary notes [J]. J Clin Pediatr Dent, 2003, 27(2): 95-100.
[3] Ackerman RI, Klapper L. Tongue position and open-bite: the key roles of growth and the nasopharyngeal airway [J]. ASDC J Dent Child, 1981, 48(5): 339-345.
[4] Gaillard-Perera H, Farah A, Gaillard A. Respiratory pathology of sleep and the upper airways [J]. Rev Stomatol Chir Maxillofac, 1992, 93(2): 130-137.
[5] McNamara JA. Influence of respiratory pattern on craniofacial growth [J]. Angle Orthod, 1981, 51(4): 269-300.
[6] Harvold EP, Tomer BS, Vargervik K, et al. Primate experiments on oral respiration [J]. Am J Orthod, 1981, 79(4): 359-372.
[7] Edge D, Bradford A, Jones JF, et al. Chronic intermittent hypoxia alters genioglossus motor unit discharge patterns in the anaesthetized rat [J]. Adv Exp Med Biol, 2012, 758: 295-300.
[8] Scarano E, Fetoni AR, Picciotti P, et al. Can chronic nasal obstruction cause dysfunction of the paratubal muscles and otitis media? An experimental study in developing Wistar rats [J]. Acta Otolaryngol, 2003, 123(2): 288-291.
[9] Ryan S, Taylor CT, McNicholas WT. Systemic inflammation: a key factor in the pathogenesis of cardiovascular complications in obstructive sleep apnoea syndrome?[J]. Postgrad Med J, 2009, 85(1010): 693-698.
[10] Musso G, Olivetti C, Cassader M, et al. Obstructive sleep apnea-hypopnea syndrome and nonalcoholic fatty liver disease: emerging evidence and mechanisms[J]. Semin Liver Dis, 2012, 32(1):49-64.
[11] Minchenko A, Leshchinsky I, Opentanova I, et al. Hypoxia-inducible factor-1-mediated expression of the 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-3 (PFKFB3) gene. Its possible role in the Warburg effect[J]. J Biol Chem, 2002, 277(8): 6183-6187.
[12] Royer C, Lachuer J, Crouzoulon G, et al. Effects of gestational hypoxia on mRNA levels of Glut3 and Glut4 transporters, hypoxia inducible factor-1 and thyroid hormone receptors in developing rat brain [J]. Brain Res, 2000, 856(1-2): 119-128.
[13] Yang X, Wei A, Liu Y, et al. IGF-1 protects retinal ganglion cells from hypoxia-induced apoptosis by activating the Erk-1/2 and Akt pathways [J]. Mol Vis, 2013, 19: 1901-1912.
[14] Kovaleva IuO, Artem'eva MM, Medvedev OS, et al. Chronic administration of estrogen receptors antagonist reduces degree of hypoxia-induced pulmonary hypertension caused by chronic injections of estrogen in ovariectomised female Wistar rats [J]. Eksp Klin Farmakol, 2013, 76(7): 15-18.
[15] Vijayasarathy C, Damle S, Prabu SK, et al. Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia [J]. Eur J Biochem, 2003, 270(5): 871-879.