上海口腔医学 ›› 2022, Vol. 31 ›› Issue (5): 517-522.doi: 10.19439/j.sjos.2022.05.013

• 论著 • 上一篇    下一篇

口腔鳞状细胞癌组织中RACK1与M2/M1联合检测与预后的相关性分析

朱江1, 许腾2, 宋冬惠1, 吴森斌1   

  1. 1.南通大学附属医院 口腔科, 江苏 南通 226000;
    2.南京医科大学附属口腔医院 口腔颌面外科,江苏 南京 210005
  • 收稿日期:2021-07-29 修回日期:2021-09-27 出版日期:2022-10-25 发布日期:2022-11-01
  • 通讯作者: 朱江,E-mail:zhujiang15910@163.com
  • 作者简介:朱江(1988-),男,硕士,主治医师
  • 基金资助:
    江苏省自然科学基金(2019489); 南通市科技局课题(JCZ19112)

Study on the correlation between combined detection of RACK1, M2/M1in oral squamous cell carcinoma, prognosis of the patients

ZHU Jiang1, XU Teng2, SONG Dong-hui1, WU Sen-bin1   

  1. 1. Department of Stomatology, Affiliated Hospital of Nantong University. Nantong 226000;
    2. Department of Oral and Maxillofacial Surgery, Stomatological College of Nanjing Medical University. Nanjing 210005, Jiangsu Province, China
  • Received:2021-07-29 Revised:2021-09-27 Online:2022-10-25 Published:2022-11-01

摘要: 目的: 探讨口腔鳞状细胞癌(OSCC)组织中活性蛋白激酶C受体1(RACK1)与M2和M1型巨噬细胞的比值(M2/M1)联合检测与预后的关系。方法: 选择2017年2月—2018年5月南通大学附属医院收治的OSCC患者129例,检测并对比癌组织、癌旁组织中RACK1及M2/M1情况,分析癌组织中RACK1表达、M2/M1与临床病理因素及预后的关系。采用SPSS 18.0软件包对数据进行统计学分析。结果: 癌组织中RACK1阳性表达率、M2/M1值显著高于癌旁组织(P<0.05)。Ⅲ~Ⅳ期、有淋巴结转移、累及脉管患者癌组织中RACK1阳性表达率分别高于Ⅰ~Ⅱ期、无淋巴结转移、无累及脉管患者(P<0.05),Ⅲ~Ⅳ期、低分化、有淋巴结转移患者癌组织中M2/M1值显著高于Ⅰ~Ⅱ期、中高分化、无淋巴结转移患者(P<0.05)。Cox回归分析显示,肿瘤分期、淋巴结转移、RACK1阳性表达率、M2/M1是影响总生存率的独立预后因素(P<0.05)。ROC曲线显示,癌组织RACK1、M2/M1及两者联合预测OSCC患者预后的AUC分别为0.743、0.718、0.875。RACK1阳性表达患者生存率为62.24%,RACK1阴性表达患者生存率为92.31%;RACK1阳性与阴性表达患者的总生存率曲线相比,差异有统计学意义(P<0.05)。M2/M1≥2.06患者的存活率为61.70%,M2/M1<2.06患者的生存率为88.24%;M2/M1≥2.06与M2/M1<2.06患者的总生存率曲线相比,差异有统计学意义(P<0.05)。结论: OSCC患者癌组织中RACK1、M2/M1异常高表达,并与患者病理情况和预后相关,两者联合预测患者预后具有一定效能。

关键词: 口腔, 鳞状细胞癌, 活性蛋白激酶C受体1, M2型巨噬细胞, M1型巨噬细胞, 预后

Abstract: PURPOSE: To explore the relationship between combined detection of tissue active protein kinase C receptor 1 (RACK1) and M2/M1 and the prognosis of oral squamous cell carcinoma (OSCC). METHODS: 129 OSCC patients admitted to the Affiliated Hospital of Nantong University were selected as the research subjects from February 2017 to May 2018. RACK1 and M2/M1 in cancer tissues and adjacent tissues of patients were detected and compared. The relationship between RACK1 expression, M2/M1 and clinicopathological factors, and relationship between RACK1 expression, M2/M1 and prognosis in cancer tissue were analyzed. SPSS 18.0 software package was used for data analysis. RESULTS: The positive expression rate of RACK1 and the value of M2/M1 in cancer tissues were significantly higher than those in adjacent tissues (P<0.05). The positive expression rate of RACK1 in cancer tissues of patients with stage Ⅲ-Ⅳ, cervical lymph node metastasis, and vascular involvement were significantly higher than those with stage Ⅰ-Ⅱ, no lymph node metastasis, and no vascular involvement(P<0.05). M2/M1 values in cancer tissues of patients with stage Ⅲ-Ⅳ, poor differentiation, and cervical lymph node metastasis were significantly higher than those with stage Ⅰ-Ⅱ, moderate differentiation, and no cervical lymph node metastasis(P<0.05). Cox regression analysis showed that the stage of tumor, cervical lymph node metastasis, positive expression rate of RACK1, and M2/M1 were independent prognostic factors affecting overall survival (P<0.05). ROC curve showed that AUC of cancer tissue RACK1, M2/M1 and combination of the two to predict the prognosis of OSCC patients were 0.743, 0.718 and 0.875, respectively. The survival rate of patients with positive expression of RACK1 was 62.24%, and the survival rate of patients with negative expression of RACK1 was 92.31%. The overall survival rate of patients with positive expression of RACK1 and patients with negative expression were compared, and the difference was statistically significant(P<0.05). The survival rate of patients with M2/M1≥2.06 was 61.70%, the survival rate of patients with M2/M1<2.06 was 88.24%, and the overall survival curve of patients with M2/M1≥2.06 was compared with that of patients with M2/M1<2.06, and the difference was statistically significant(P<0.05). CONCLUSIONS: The expression of RACK1 and M2/M1 is abnormally high in cancer tissues of OSCC patients. The expression of RACK1 and M2/M1 is related to the patient's pathology and prognosis. The combination of the two has certain efficacy in predicting the prognosis of OSCC patients.

Key words: Oral cavity, Squamous cell carcinoma, Active protein kinase C receptor 1, M2 type macrophages, M1 type macrophages, Prognosis

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