上海口腔医学

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (1): 100-103.doi: 10.19439/j.sjos.2022.01.021

• 论著 • 上一篇    下一篇

慢性牙周炎合并慢性阻塞性肺疾病患者牙龈卟啉单胞菌rag位点基因分析

于淼1, 李倜1, 刘婷2, 贺龙飞1, 田甜甜1, 陈静3   

  1. 1.潍坊市人民医院 口腔科,2.呼吸与危重症医学科,山东 潍坊 261000;
    3.青岛市黄岛区中心医院 口腔科,山东 青岛 266500
  • 收稿日期:2021-04-29 修回日期:2021-06-16 出版日期:2022-02-25 发布日期:2022-03-10
  • 通讯作者: 陈静,E-mail：cj283@163.com
  • 作者简介:于淼（1982-）,女,博士,E-mail：dentistyum@163.com
  • 基金资助:
    山东省卫生健康科技发展项目 （2019WS250）; 潍坊市卫生健康委科研项目（wfwsjk_2019_034）

Detection of Porphyromonas gingivalis rag genotypes in patients of chronic periodontitis with chronic obstructive pulmonary disease

YU Miao1, LI Ti1, LIU Ting2, HE Long-fei1, TIAN Tian-tian1, CHEN Jing3   

  1. 1. Department of Stomatology, 2. Department of Plumonary and Critical Care Medicine, Weifang People's Hospital. Weifang 261000;
    3. Department of Stomatology, Qingdao Huangdao District Central Hospital. Qingdao 266500, Shandong Province, China
  • Received:2021-04-29 Revised:2021-06-16 Online:2022-02-25 Published:2022-03-10

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摘要： 目的: 分析不同 rag 基因型牙龈卟啉单胞菌在慢性牙周炎合并慢性阻塞性肺疾病（chronic obstructive pulmonary disease, COPD）患者中的分布状况。方法: 选择慢性牙周炎及合并COPD的慢性牙周炎患者各30例,收集唾液样本,采用16S rDNA 聚合酶链反应（PCR）法检测牙龈卟啉单胞菌的检出率及rag基因型,采用SPSS 22.0软件包进行统计学分析。结果: 合并COPD的慢性牙周炎患者唾液中牙龈卟啉单胞菌阳性检出率为76.67%,慢性牙周炎组为63.33%,组间差异无统计学意义（P>0.05）;rag-1基因型的检出率分别为70%和30.77%,差异显著（P<0.05）,rag-2、rag-3和rag-4的检出率无统计学差异。结论: 合并COPD的慢性牙周炎患者唾液中牙龈卟啉单胞菌的rag 基因型具有多态性,其中,rag-1型的牙龈卟啉单胞菌与COPD的发展关系密切。

关键词: 牙周炎, 慢性阻塞性肺疾病, 牙龈卟啉单胞菌, 基因型, rag 基因

Abstract: PURPOSE: To investigate the distribution of Porphyromonas gingivalis(P.g) rag genotypes in patients of chronic periodontitis with chronic obstructive pulmonary disease (COPD). METHODS: Thirty patients with chronic periodontitis and 30 patients with chronic periodontitis complicated with COPD were included. Saliva samples were collected from all subjects. The detection rate and rag genotype of P.g in saliva were detected by 16S rDNA polymerase chain reaction (PCR). SPSS 22.0 software package was used for statistical analysis. RESULTS: The positive rate of P.g was 76.67% in chronic periodontitis patients with COPD, and 63.33% in chronic periodontitis group, there was no significant difference between the two groups (P>0.05). The detection rates of rag-1 genotype in the two groups were 70% and 30.77%, respectively, there was significant difference between the two groups(P<0.05). The detection rates of rag-2, rag-3 and rag-4 in the two groups were not significantly different. CONCLUSIONS: Various rag genotypes can be found in patients of chronic periodontitis with COPD. Rag-1 might have more close correlation with the development of COPD.

Key words: Periodontitis, Chronic obstructive pulmonary disease, Porphyromonas gingialis, Genotype, Rag gene

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