上海口腔医学 ›› 2021, Vol. 30 ›› Issue (6): 573-578.doi: 10.19439/j.sjos.2021.06.003

• 论著 • 上一篇    下一篇

Met激酶抑制剂BMS-777607对舌鳞癌细胞CAL27增殖、凋亡的影响

马曌磊1, 刘佳楠1, 苏荣健2, 贺武斌3, 黄克强1   

  1. 1.锦州医科大学附属第二医院·口腔医学院,辽宁 锦州 121004;
    2.锦州医科大学 生命科学研究院,辽宁 锦州 121001;
    3.锦州医科大学附属第一医院,辽宁 锦州 121001
  • 收稿日期:2020-04-21 修回日期:2020-05-28 发布日期:2022-03-09
  • 通讯作者: 黄克强,E-mail:hkq9@163.com
  • 作者简介:马曌磊(1991-),女,硕士研究生, E-mail:410238376@qq.com
  • 基金资助:
    辽宁省自然科学基金(2015020326)

Effect of Met kinase inhibitor BMS-777607 on proliferation and apoptosis of tongue cell line CAL 27 squamous cell carcinoma

MA Zhao-lei1, LIU Jia-nan1, SU Rong-jian2, HE Wu-bin3, HUANG Ke-qiang1   

  1. 1. The Second Affiliated Hospital and College of Stomatology of Jinzhou Medical University. Jinzhou 121004;
    2. Institute of Life Sciences, Jinzhou Medical University. Jinzhou 121001;
    3. The First Affiliated Hospital of Jinzhou Medical University. Jinzhou 121001, Liaoning Province, China
  • Received:2020-04-21 Revised:2020-05-28 Published:2022-03-09

摘要: 目的: 探讨Met激酶抑制剂BMS-777607对人舌鳞状细胞癌(鳞癌)CAL27细胞增殖、凋亡的影响。方法: 采用MTT、克隆形成实验及EdU细胞成像实验,检测BMS-777607对CAL27细胞增殖的影响。通过Heochst33342染色,观察BMS-777607对CAL27细胞的凋亡形态变化。采用JC-1染色,检测BMS-777607对CAL27细胞线粒体膜电位变化。采用Western免疫印迹检测BMS-777607对CAL27细胞中增殖蛋白Akt、p-Akt,凋亡相关蛋白Bcl-2、Cleaved caspase-3、Bax、Parp表达的影响。采用SPSS 22.0软件包对数据进行统计学分析。结果: BMS-777607抑制CAL27细胞增殖,促进其凋亡,呈浓度依赖性(P<0.05)。BMS-777607抑制Bcl-2、p-Akt表达,促进Bax、Cleaved caspase-3、Parp蛋白表达(P<0.05)。结论: BMS-777607能够抑制CAL27细胞增殖并促进其凋亡。

关键词: Met激酶, BMS-777607, 舌鳞癌, CAL27细胞, 凋亡

Abstract: PURPOSE: To investigate the effect of Met kinase inhibitor BMS-777607 on proliferation and apoptosis of tongue squamous cell carcinoma cell line CAL27. METHODS: The effect of BMS-777607 on proliferation of CAL27 was detected by MTT method, clone formation assay and EdU cell imaging. Morphological changes of apoptosis of CAL27 cells induced by BMS-777607 were observed by Heochst33342 staining. JC-1 staining was used to detect the changes of mitochondrial membrane potential of CAL27 cells treated with BMS-777607. Western blot was used to detect the effect of BMS-777607 on the expression of proliferation protein Akt, p-Akt and apoptosis-related proteins Bcl-2, Cleaved caspase-3, Bax and Parp in CAL27 cells. The data were analyzed using SPSS 22.0 software package. RESULTS: BMS-777607 inhibited proliferation and promoted apoptosis of CAL27 cells in a concentration-dependent manner(P<0.05). It also inhibited the expression of Bcl-2 and p-Akt and promoted the expression of Bax, Cleaved caspase-3 and Parp protein (P<0.05). CONCLUSIONS: BMS-777607 can inhibit proliferation and promote apoptosis of CAL27 cells.

Key words: Met kinase, BMS-777607, Tongue squamous cell carcinoma, CAL27, Apoptosis

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