人骨髓间充质干细胞对舌鳞癌细胞系Cal-27侵袭作用的影响

doi:10.19439/j.sjos.2020.02.009

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (2): 155-161.doi: 10.19439/j.sjos.2020.02.009

人骨髓间充质干细胞对舌鳞癌细胞系Cal-27侵袭作用的影响

徐竹青^1,2, 张馨予¹, 王少如³, 李涛², 董刚², 李凤梅², 郑建金², 王云英⁴, 徐晓娜⁴

1.青岛大学口腔医学院,山东青岛 266000;
2.青岛市市立医院口腔医学中心,山东青岛 266000;
3.大连医科大学,辽宁大连 116044;
4.青岛市市立医院基础实验室,山东青岛 266000

收稿日期:2018-12-28 修回日期:2019-04-13 出版日期:2020-04-25 发布日期:2020-04-30
通讯作者: 郑建金,E-mail:zhjj19631016@sina.com
作者简介:徐竹青(1994-),女,在读硕士研究生,E-mail:951367150@qq.com
基金资助:
青岛市科技局重点项目(15-9-2-79-nsh)

Effect of human bone marrow mesenchymal stem cells on invasion of tongue squamous cell carcinoma cell line Cal-27

XU Zhu-qing^1,2, ZHANG Xin-yu¹, WANG Shao-ru³, LI Tao², DONG Gang², LI Feng-mei², ZHENG Jian-jin², WANG Yun-ying⁴, XU Xiao-na⁴

1.School of Stomatology, Qingdao University.Qingdao 266000, Shandong Province;
2.Qingdao City Hospital Stomatological Medical Center.Qingdao 266000, Shandong Province;
3.Dalian Medical University. Dalian 116044, Liaoning Province;
4.Basic Laboratory of Qingdao Municipal Hospital.Qingdao 266000, Shandong Province, China

Received:2018-12-28 Revised:2019-04-13 Online:2020-04-25 Published:2020-04-30

摘要/Abstract

摘要： 目的探讨人骨髓间充质干细胞(human bone marrow mesenchymal stem cells,hBM-MSCs)对舌鳞癌细胞系Cal-27侵袭的影响及作用机制。方法分别培养hBM-MSCs和Cal-27,倒置显微镜下观察细胞形态。hBM-MSCs和Cal-27共培养,获得共培养后的Cal-27细胞。划痕实验观察Cal-27的迁移面积;Transwell迁移及侵袭实验观察Cal-27的迁移及侵袭细胞数目,绘制条形图。使用荧光定量PCR技术,观察hBM-MSCs对Cal-27侵袭相关肿瘤标志物E-cadherin、twist、slug、snail、MMP-2、MMP-9基因表达的影响;使用Western 免疫印迹技术,观察hBM-MSCs对Cal-27侵袭相关肿瘤标志物MMP-2、MMP-9蛋白表达的影响。采用SPSS 19.0软件包对数据进行统计学分析。结果 hBM-MSCs可促进Cal-27的侵袭,伴随E-cadherin下调,twist、slug、snail、MMP-2、MMP-9基因表达上调及MMP-2、MMP-9蛋白表达上调。结论 hBM-MSCs促进Cal-27细胞的侵袭作用,可能与其上调Cal-27细胞侵袭作用相关肿瘤标志物的表达有关。

关键词: 人骨髓间充质干细胞, 舌鳞癌细胞, 侵袭, 肿瘤标志物

Abstract: PURPOSE: To investigate the effect of human bone marrow mesenchymal stem cells (hBM-MSCs) on invasion of tongue squamous cell carcinoma cell line Cal-27 and its mechanism. METHODS: hBM-MSCs and Cal-27 were cultured respectively, and the morphology of the cells was observed under an inverted microscope. The co-cultured Cal-27 cells were obtained by co-culture of hBM-MSCs and Cal-27. The migration area of Cal-27 was observed by scratch test;transwell migration and invasion experiments were performed to observe migration and invasion of Cal-27, and a bar graph was then drawn. Fluorescence quantitative PCR was used to observe the effect of hBM-MSCs on gene expression of the tumor markers E-cadherin, twist, slug, snail, MMP-2 and MMP-9. Western blot was used to observe the effect of hBM-MSCs on protein expression of MMP-2 and MMP-9, related to the invasion of Cal-27. SPSS 19.0 software package was used for statistical analysis of the data. RESULTS: Under the influence of hBM-MSCs, the invasion of Cal-27 was promoted, accompanied by down-regulation of E-cadherin, up-regulation of twist, slug, snail, MMP-2, MMP-9 and up-regulation of MMP-2 and MMP-9 expression. CONCLUSIONS: hBM-MSCs can promote invasion of Cal-27 cells, which may be related to up-regulation of the expression of tumor markers related to invasion of Cal-27 cells.

Key words: Human bone marrow mesenchymal stem cells, Tongue squamous cell carcinoma, Invasion, Tumor markers

中图分类号:

徐竹青, 张馨予, 王少如, 李涛, 董刚, 李凤梅, 郑建金, 王云英, 徐晓娜. 人骨髓间充质干细胞对舌鳞癌细胞系Cal-27侵袭作用的影响[J]. 上海口腔医学, 2020, 29(2): 155-161.

XU Zhu-qing, ZHANG Xin-yu, WANG Shao-ru, LI Tao, DONG Gang, LI Feng-mei, ZHENG Jian-jin, WANG Yun-ying, XU Xiao-na. Effect of human bone marrow mesenchymal stem cells on invasion of tongue squamous cell carcinoma cell line Cal-27[J]. Shanghai Journal of Stomatology, 2020, 29(2): 155-161.

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人骨髓间充质干细胞对舌鳞癌细胞系Cal-27侵袭作用的影响

Effect of human bone marrow mesenchymal stem cells on invasion of tongue squamous cell carcinoma cell line Cal-27

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